Evaluation of biomarkers in caged fishes and mussels to assess the quality of waters in a bay of the NW Mediterranean Sea

Stien, Xavier; Percic, Philippe; Gnassia‐Barelli, M.; Roméo, Michèle; Lafaurie, M.

doi:10.1016/s0269-7491(98)00013-x

Cited by 117 publications

(43 citation statements)

References 26 publications

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“…These results are in agreement with those of other studies which reported that the different GST activity between males and females of fish could be caused by the reproductive cycle (George & Buchanan, 1990;Jedanski-Grymlas et al, 1995;Ruus et al, 2002). This enzyme is known also to be influenced by diet, temperature or by age (Stien et al, 1998;Ronisz et al, 1999;Ruus et al, 2002). In addition, variation in GST activity has been reported in several fish species exposed to organic pollutants under laboratory and field studies.…”

Section: Discussionmentioning

confidence: 99%

“…However, the antioxidant defense mechanisms of aquatic organisms are subjected to seasonal and biological control, because the antioxidant responses may vary during the year, depending on feeding, reproductive cycle, temperature and other factors (Stien et al, 1998;Ronisz et al, 1999;Elia etal, 2001). This leads to a limitation when field studies are performed, because the biochemical changes might be linked either to fish physiology or to exposure to contaminants.…”

Section: Introductionmentioning

confidence: 99%

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Polychlorinated biphenyls and antioxidant enzymes in liver ofCyprinus carpiofrom Lake Trasimeno

Elia

Galarini

Dörr

et al. 2005

Italian Journal of Zoology

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Polychlorinated biphenyl (PCB) congeners and selected biochemical parameters (total glutathione, glutathione-S-transferase, glutathione peroxidase, catalase, glutathione reductase, glyoxalase I, glyoxalase II were measured in carp liver of both sexes collected from Lake Trasimeno during the spawning and postspawning periods. The most abundant congeners found were PCB-138, PCB-153 and PCB-180, even if all PCB congeners investigated were recorded in low amounts. No significant variations in congener concentrations in relation to sex and sampling periods were observed in carp liver. Among the biochemical parameters investigated, total glutathione, glutathione S-transferase, glyoxalase II and catalase, showed differences according to sex, and thus the variations in antioxidant response of carp seem to be linked more to biological status, than to the presence of PCBs congeners in liver of the same specimens.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Polychlorinated biphenyls and antioxidant enzymes in liver ofCyprinus carpiofrom Lake Trasimeno

Elia

Galarini

Dörr

et al. 2005

Italian Journal of Zoology

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“…This extraordinary characteristic makes it possible to use them to predict field existence and stress induced by these chemicals (Sanchez-Hernandez et al, 1998;Stien et al, 1998;Cajaraville et al, 2000;den Besten et al, 2001;Abdel-Halim et al, 2006;Denoyelle et al, 2007;Sáenz et al, 2010;van Oosterom et al, 2010;Printes et al, 2011). To this end, the "normal" activity of ChE should first be confirmed.…”

Section: Introductionmentioning

confidence: 99%

Developing antibodies from cholinesterase derived from prokaryotic expression and testing their feasibility for detecting immunogen content in Daphnia magna

Liu

Yuan

2016

J. Zhejiang Univ. Sci. B

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Abstract:To yield cholinesterase (ChE) from prokaryotic expression, the ChE gene that belongs to Daphnia magna was amplified by reverse transcription-polymerase chain reaction (RT-PCR) using forward primer 5'-CCCYGGNGCSAT GATGTG-3' and reverse primer 5'-GYAAGTTRGCCCAATATCT-3'. To express the gene, one sequence of the amplified DNA, which was able to encode a putative protein containing two conserved carboxylesterase domains, was connected to the prokaryotic expression vector PET-29a(+). The recombinant vector was transformed into Escherichia coil BL21 (DE3). Protein expression was induced by isopropy-D-thiogalactoside. The expressed ChE was used as an immunogen to immunize BALB/c mice. The obtained antibodies were tested for their specificity towards crude enzymes from species such as Alona milleri, Macrobrachium nipponense, Bombyx mori, Chironomus kiiensis, Apis mellifera, Eisenia foetida, Brachydanio rerio, and Xenopus laevis. Results indicated that the antibodies had specificity suitable for detecting ChE in Daphnia magna. A type of indirect and non-competitive enzyme-linked immunosorbent assay (IN-ELISA) was used to test the immunoreactive content of ChE (ChE-IR) in Daphina magna. The detection limit of the IN-ELISA was found to be 14.5 ng/ml at an antiserum dilution of 1:22 000. Results from tests on Daphnia magna exposed to sublethal concentrations of triazophos indicated a maximal induction of 57.2% in terms of ChE-IR on the second day after the animals were exposed to a concentration of 2.10 μg/L triazophos. Testing on animals acclimatized to a temperature of 16 °C indicated that ChE-IR was induced by 16.9% compared with the ChE-IR content detected at 21 °C, and the rate of induction was 25.6% at 10 °C. The IN-ELISA was also used to test the stability of ChE-IR in collected samples. Repeated freezing and thawing had no influence on the outcome of the test. All these results suggest that the polyclonal antibodies developed against the recombinant ChE are as efficient as those developed against the native ChE in detecting ChE content in Daphnia magna.

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“…Regardless the types, ChEs are strongly inhibited by anticholinesterases such as organophosphates (OPs) and cabamates (CBs). This allows them to serve as effective biomarkers in the field (SanchezHernandez et al, 1998;Stien et al, 1998;Kirby et al, 2000;den Besten et al, 2001;Forget et al, 2003;Quintaneiro et al, 2006;Jung et al, 2008;Elhalwagy et al, 2010;Sáenz et al, 2010;van Oosterom et al, 2010;Printes et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Purification and studies on characteristics of cholinesterases from Daphnia magna

Yang

Niu

2013

J. Zhejiang Univ. Sci. B

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Due to their significant value in both economy and ecology, Daphnia had long been employed to investigate in vivo response of cholinesterase (ChE) in anticholinesterase exposures, whereas the type constitution and property of the enzyme remained unclear. A type of ChE was purified from Daphnia magna using a three-step procedure, i.e., Triton X-100 extraction, ammonium sulfate precipitation, and diethylaminoethyl (DEAE)-Sepharose™-Fast-Flow chromatography. According to sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), molecular mass of the purified ChE was estimated to be 84 kDa. Based on substrate studies, the purified enzyme preferred butyrylthiocholine iodide (BTCh) [with maximum velocity (V max )/Michaelis constant (K m )=8.428 L/(min·mg protein)] to acetylthiocholine iodide (ATCh) [with V max /K m =5.346 L/(min·mg protein)] as its substrate. Activity of the purified enzyme was suppressed by high concentrations of either ATCh or BTCh. Inhibitor studies showed that the purified enzyme was more sensitive towards inhibition by tetraisopropylpyrophosphoramide (iso-OMPA) than by 1,5-bis(4-allyldimethylammoniumphenyl) pentan-3-one dibromide (BW284C51). Result of the study suggested that the purified ChE was more like a type of pseudocholinesterase, and it also suggested that Daphnia magna contained multiple types of ChE in their bodies.

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Evaluation of biomarkers in caged fishes and mussels to assess the quality of waters in a bay of the NW Mediterranean Sea

Cited by 117 publications

References 26 publications

Polychlorinated biphenyls and antioxidant enzymes in liver ofCyprinus carpiofrom Lake Trasimeno

Polychlorinated biphenyls and antioxidant enzymes in liver ofCyprinus carpiofrom Lake Trasimeno

Developing antibodies from cholinesterase derived from prokaryotic expression and testing their feasibility for detecting immunogen content in Daphnia magna

Purification and studies on characteristics of cholinesterases from Daphnia magna

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