Polychlorinated biphenyls and antioxidant enzymes in liver of<i>Cyprinus carpio</i>from Lake Trasimeno

Oxytetracycline (OTC) is employed in fish farms to contest or prevent bacterial infections. We simulated an OTC treatment at therapeutic level (75 mg kg(-1)) and at higher doses (150, 300 mg kg(-1)) for 10 days. A withdrawal period of 10 days was considered for treated carp, carrying out the same chemical and biochemical analyses (total glutathione, superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase, glutathione S-transferase and malondialdehyde). The aim was to obtain data related to the carryover in muscle and on variations in the antioxidant indicators in liver and kidney. The OTC residual levels in muscle showed a dose-response relationship. After 10 days of treatment at the recommended dose (75 mg kg(-1)), the mean value in muscle was 295 μg kg(-1). After 10 withdrawal days, residues in all treated groups were not entirely eliminated by fish. Residues of recommended 75 mg kg(-1) OTC dose were lower than the maximum permitted by EEC regulation: 100 μg kg(-1). Disturbance in the antioxidant systems in liver and kidney was recorded in (150, 300 mg kg(-1)) carp, as well as during the withdrawal period. A lowered superoxide dismutase activity and higher levels of catalase, glutathione peroxidase, glutathione reductase and glutathione were evaluated in liver, while in kidney only higher malondialdehyde and glutathione S-transferase concentrations were recorded for 300 mg kg(-1) dose. The therapeutic OTC dose exerted lower effects, and only in liver, enhancement of GPx and GR activities was recorded. After the withdrawal period, altered antioxidant responses in tissues were restored for all three OTC doses.

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Seasonal variability of detoxificant response and heavy metal accumulation in tissues of both sexes in Tinca tinca (L.) from Lake Trasimeno

Elia

Dörr

Abete³

et al. 2009

Rev Fish Biol Fisheries

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Tinca tinca were sampled seasonally from Lake Trasimeno in order to investigate the influence of heavy metal accumulation and changing environmental parameters on the antioxidant responses in this species. Liver, gills and kidney of both sexes were analyzed for total glutathione, catalase, glutathione peroxidase, glutathione reductase, glutathione S-transferase, glyoxalase I, glyoxalase II, while muscle was analyzed for arsenic, lead, cadmium and mercury. The MRLs (maximum residue limits) set by actual regulation were not exceeded for heavy metals, but a seasonal and sexlinked variability of antioxidant parameters was observed, and major variations occurred especially in summer and autumn. In this shallow lake, heavy metal accumulation did not affect the biochemical variations, which are rather due to the decreased water level inducing hyperoxia and hypoxia within 24 h, high temperature and loss of mating and refuge areas.

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Polychlorinated biphenyls and antioxidant enzymes in liver ofCyprinus carpiofrom Lake Trasimeno

Cited by 5 publications

References 38 publications

Biochemical Effects of Heavy Metals and Organochlorine Compounds Accumulated in Different Tissues of Yellow-Legged Gulls (Larus Michahellis)

Biochemical Effects of Heavy Metals and Organochlorine Compounds Accumulated in Different Tissues of Yellow-Legged Gulls (Larus Michahellis)

Transferability of oxytetracycline (OTC) from feed to carp muscle and evaluation of the antibiotic effects on antioxidant systems in liver and kidney

Seasonal variability of detoxificant response and heavy metal accumulation in tissues of both sexes in Tinca tinca (L.) from Lake Trasimeno

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