Evaluation of bioactive secondary metabolites from endophytic fungus Pestalotiopsis neglecta BAB-5510 isolated from leaves of Cupressus torulosa D.Don

Sharma, Deeksha; Pramanik, Avijit; Agrawal, P. K.

doi:10.1007/s13205-016-0518-3

Cited by 153 publications

(90 citation statements)

References 65 publications

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“…All of the endophyte cultures were deposited at Center of Excellence in Fungal Research Mae Fah Luang University. All isolated endophytic fungi were characterised according to the modified method of Sharma et al (2016), including microscopic observation of mycelia, asexual/sexual spores and colony morphology.…”

Section: Methodsmentioning

confidence: 99%

Antibacterial secondary metabolites from an endophytic fungus, Arthrinium sp. MFLUCC16-1053 isolated from Zingiber cassumunar

Pansanit

Pripdeevech

2018

Mycology

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Forty-four endophytes were isolated from Zingiber cassumunar and identified morphologically. The ethyl acetate extracts of all endophytes were obtained. The ethyl acetate extracts were subjected to study antibacterial and antioxidant activities. The ethyl acetate extract of the Arthrinium sp. MFLUCC16-1053 showed activity against both gram-positive and gram-negative bacteria. Specifically, the minimum inhibition concentration against Staphylococcus aureus and Escherichia coli was 31.25 and 7.81 µg/mL, respectively. In addition, the crude extract showed highest antioxidant activity in 2,2-diphenyl-1-picrylhydrazyl scavenging at IC50 value of 28.47 µg/mL. Gas chromatography-mass spectrometry analysis revealed that the extract of the Arthrinium sp. MFLUCC16-1053 contains various antibacterial and antioxidant compounds which are β-cyclocitral, 3E-cembrene A, laurenan-2-one, sclareol, 2Z,6E-farnesol, cembrene, β-isocomene and γ-curcumene. The fungus Arthrinium sp. MFLUCC16-1053 was also identified by molecular and phylogenetic methods.

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Section: Methodsmentioning

confidence: 99%

Antibacterial secondary metabolites from an endophytic fungus, Arthrinium sp. MFLUCC16-1053 isolated from Zingiber cassumunar

Pansanit

Pripdeevech

2018

Mycology

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“…Over time, some endophytic fungi have developed the ability to produce the same or similar bioactive substances to those produced by host plants (Sharma et al, 2016). This lends a great advantage to the study of the relationships between endophytes and their host plants (Fouda et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Secondary metabolites from endophytic fungus from Lippia sidoides Cham.

Ferreira¹,

Santos²,

Soares³

et al. 2017

J. Med. Plants Res.

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Lippia sidoidesCham. (Verbenaceae) is a species native to the Brazilian northeast, widely used in popular medicine. Its leaves were used for the isolation of endophytic fungi and extraction of metabolites. Among them, three were selected according to fungitoxicity tests against the maize phytopathogenic fungus, Curvularia lunata (Wakker). However, the objective of this study was to identify the role of L. sidoides extracts associated with their endophytic fungi, necessary to reduce excess of fungicides applied on the maize crop. Metabolites were evaluated for antioxidant activity by 2.2-diphenyl-1-picrylhydrazyl (DPPH), phenols, total flavonoids and one of it endophytic fungus were evaluated for synergism (Verticilium sp. and plant extracts). The endophytic fungi and plant extracts evaluated for phenolic content ranged from 0.29 ± 0.05 to 96.94 ± 11.86 mgEAG/g, the content of flavonoids from 14.31 ± 1.56 to 192.33 ± 4.58 mgER/g, and antioxidant activity could only be observed for the plant extract with EC 50 81 ± 0.3%. The secondary metabolites identified by HPLC in the plant extract were catechin, quercetin, gallic acid and naringin. Naringenin, catechin, epigallocatechin gallate and quercetin were identified in the extract of the fungi viz. Verticillium sp. and Fusarium sp. Synergistic analysis between a 1:1 proportion of plant and fungal extracts has shown more efficient (79.0%) inhibition of C. lunata. Thus, alternative control of phytopathogenic fungi can be accomplished using plant extracts associated with their endophytic fungi, reducing the excess of fungicides applied on the maize crop.

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“…Altogether, six human pathogenic-bacteria and one human yeast-pathogen were used in an agar-well diffusion (AWD) assay, to determine the antimicrobial activity of the crude extracts obtained from the fungal endophytes [11, 18, 20]. Stocks (10 mg/ml) for both the intracellular and extracellular-extracts were made using DMSO as a solvent.…”

Section: Methodsmentioning

confidence: 99%

Annulohypoxylonsp. strainMUS1, an Endophyte isolated fromTaxus wallichianaZucc. produces Taxol and Other Bioactive Metabolites

Gauchan

Vélëz

Acharya

et al. 2020

Preprint

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24Endophytes are microbial colonizers that reside in plants by symbiotic association produces 25 several biological classes of natural products. The current study focuses on the isolation and 26 characterization of bioactive compounds produced by endophytic fungi isolated from the 27 Himalayan yew (Taxus wallichiana) collected from the Mustang district of Nepal. The plant 28 materials were collected from the Lower-Mustang region in the month of October 2016 and the 29 endophytic fungi were isolated on artificial media from inner tissues of bark and needles. 30 Antimicrobial and antioxidant activity, along with total phenolic-and flavonoid-content assays, 31 were used in the evaluation of bioactivity of the fermented crude extracts along with the in vitro 32 ability of the endophytes to produce the anticancer compound Taxol was analyzed. A total of 16 33 fungal morphotypes were obtained from asymptomatic inner tissues of the bark and needles of T. 34 wallichiana. Among the 16 isolates, the ethyl acetate (EA) fraction of isolate MUS1, showed 35 antibacterial and antifungal activity against all test-pathogens used, with significant inhibition 36 against Pseudomonas aeruginosa ATCC 27853 (MIC: 250 µg/ml) and the pathogenic yeast, 37 Candida albicans (MIC: 125 µg/ml). Antioxidant activity was also evaluated using 2,2-diphenyl-38 1-picrylhydrazyl (DPPH). At a concentration of 100 µg/ml, the % radical scavenging activity 39 was 83.15±0.40, 81.62±0.11, and 62.36±0.29, for ascorbic acid, butylated hydroxytoluene (BHT) 40 and the EA fraction of MUS1, respectively. The DPPH-IC50 value for the EA fraction was 81.52 41 µg/ml, compared to BHT (62.87 µg/ml) and ascorbic acid (56.15 µg/ml). The total phenolic and 42 flavonoid content in the EA fraction were 16.90±0.075 µg gallic acid equivalent (GAE) and 43 11.59±0.148 µg rutin equivalent (RE), per mg of dry crude extract, respectively. Isolate MUS1, 44 identified as an Annulohypoxylon sp. by ITS sequencing, also produced Taxol (282.05 µg/L) as 45 shown by TLC and HPLC analysis. Having the ability to produce antimicrobial and antioxidant 46 3 compounds, as well as the anticancer compound Taxol, makes Annulohypoxylon sp. strain 47 MUS1, a promising candidate for further study given that naturally occurring bioactive 48 compounds are of great interest to the pharmacological, food and cosmetic industries.49

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Evaluation of bioactive secondary metabolites from endophytic fungus Pestalotiopsis neglecta BAB-5510 isolated from leaves of Cupressus torulosa D.Don

Cited by 153 publications

References 65 publications

Antibacterial secondary metabolites from an endophytic fungus, Arthrinium sp. MFLUCC16-1053 isolated from Zingiber cassumunar

Antibacterial secondary metabolites from an endophytic fungus, Arthrinium sp. MFLUCC16-1053 isolated from Zingiber cassumunar

Secondary metabolites from endophytic fungus from Lippia sidoides Cham.

Annulohypoxylonsp. strainMUS1, an Endophyte isolated fromTaxus wallichianaZucc. produces Taxol and Other Bioactive Metabolites

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