Evaluation of Binding and Origins of Specificity of 9-Ethyladenine Imprinted Polymers

Abstract

An investigation of 9-ethyladenine (9-EA) imprinted polymers has provided insight into the origins of binding and recognition by imprinted polymers. Porogens and polymerization temperature are important variables during the polymerization. Factors that influence rebinding of the 9-EA imprinted polymer include pH and utilization of the porogen as mobile phase. Finally, quantitative structure−binding relationship studies elicited the substructures of 9-EA primarily responsible for creating the binding sites within the polymers.

Evaluation of the recognition ability of molecularly imprinted materials by surface plasmon resonance (SPR) spectroscopy

“…In the present study, the nucleic acid component adenosine was adopted as a model target molecule, since recognition of nucleic acid components is of interest and importance in connection with biosensors, drug therapy, genetic engineering, and so forth. Pioneering studies on recognition and transport of nucleic acid components have been investigated by using molecularly imprinted membranes [26][27][28][29].T o this end, recognition sites towards adenine were constructed by adopting 9-ethyladenine (9-EA) as a print molecule. The recognition of adenosine/guanosine (As/Gs) was studied as a model mixture.…”

Microfluidic Approach for the Synthesis of Micro- or Nanosized Molecularly Imprinted Polymer Particles

“…Since MIP's particle sizes are inversely proportional to the affinity and thus are directly related to the affinity functions, we demonstrate a microfluidic approach to synthesize small MIP's particles [12].…”

“…MIP materials can be provided by a molecular imprinting technique, which is a general protocol for the creation of synthetic receptor sites to detect specific organic molecules in cross-linked network polymers. molecule, 9-ethyl adenine, were combined to form prepolymerization complex [12]. 9-ethyl adenine(9-EA) was synthesized; a mixture of adenine (3.00 g, 22.2 mmol), K 2 CO 3 (6.14 g, 44.4 mmol), and ethyl bromide (3.32 mL, 44.4 mmol) was stirred in DMF (60 mL) for 24 hours under nitrogen atmosphere.…”

New Biosorbent Materials: Selectivity and Bioengineering Insights

“…Therefore, we cannot base on these models in order to compare the possible changes of distributions. A possible solution for the latter is the analysis of limiting slopes according to scatchard plots, which can be characterized as type of bi-Langmuir equation [90]. Hence, the comparison of binding properties of different or even of the same MIP by homogeneous models is difficult [78].…”