Evaluation of the recognition ability of molecularly imprinted materials by surface plasmon resonance (SPR) spectroscopy

Abstract

Polysulfone with an oligopeptide derivative of glutamyl residues (PSf-E 5.8 ) was evaluated as a candidate material for molecular recognition. PSf-E 5.8 was prepared from the N-carboxyanhydride of ␥-benzyl-l-glutamate (Glu(OBzl)-NCA) initiated by aminomethylated polysulfone (PSf). Films with molecular recognition sites with preference to adenosine (As) were prepared from PSf-E 5.8 by an alternative molecular imprinting method using 9-ethyladenine (9-EA) as a print molecule. The molecular recognition phenomena were studied by surface plasmon resonance (SPR) spectroscopy. The apparent affinity constant of the molecular recognition sites toward As was dependent on the imprinting condition and was increased from 1.30 × 10 4 to 1.60 × 10 4 mol −1 dm 3 with the decrease in the molecular imprinting ratio from 1.0 to 0.25. From the present study, it was shown that SPR is a convenient and facile method to detect molecular recognition interactions.

Chiral separation with molecularly imprinted polysulfone-aldehyde derivatized nanofiber membranes☆

“…In the previous study [27,31], the affinity constant was observed to increase with the decrease in molecular imprinting ratio; in other words, at the lower molecular imprinting ratio, more functional groups in the candidate polymer interacted with one print molecule, resulting in a higher affinity constant. Similar phenomena were also observed in the present study, and the affinity constants for the molecularly imprinted nanofiber membranes gave higher values than those of the usual molecularly imprinted membranes.…”

Modified polysulfones. VI. Preparation of polymer membrane materials containing benzimine and benzylamine groups as precursors for molecularly imprinted sensor devices

“…On the bisphenol segment, H 3,5 and H 2,6 remain largely unchanged. Benzyl protons H [15][16][17] are all within 0.2 ppm of one another, the furthest downfield being H 15 , which is affected by the electronic effect of the imine group. The H 17 1 H triplet is coupled with H 16 .…”

“…The molecular recognition of the prepared films toward the target molecule adenosine (As) was evaluated by surface plasmon resonance (SPR) spectroscopy. 17 During the measurement, a 0.02 wt % NaN 3 aqueous buffer was passed over the molecularly imprinted material surface at 5 mm 3 min…”

“…26 In the preliminary experiments, 9-EA was adopted as a print molecule for the conversion of the oligopeptide of Glu(OBzl)-NCA into molecular recognition sites toward As, and their molecular recognition ability was evaluated with SPR spectroscopy. 17 In this study, for the molecular imprinting condition, the ratio of the amount of 9-EA to that of the oligopeptide was fixed at unity. The sensor device prepared in the presence of 9-EA as a print molecule recognized As in preference to Gs.…”

Surface plasmon resonance studies on molecularly imprinted films

“…Molecular Figure 7 Affinity constants between As and molecular recognition sites converted from various candidate materials. recognition sites toward As have been previously constructed from H-Asp(OcHex)-Ile-Asp(OcHex)-Glu(OBzl)-O-CH 2 -tetrapeptide derivative (DIDE), 30 polysulfone with oligopeptide derivative of glutamyl residues (PSf-E 5.8 ), 31 cellulose acetate (CA) with 40% acetyl content, 30 carboxylated polysulfone (PSf-COOH) with the degree of substitution of 0.88, 30 Torlon (polyamide-imide), 32 hydrophilic water-soluble proteins, 19 and membrane proteins 33 from Geobacillus thermodenitrificans DSM465. The affinity constants between As and the molecular recognition sites converted from those candidate materials are summarized in Figure 7 together with the chemical structures of those materials for convenience.…”