Evaluation of bedmaking-related airborne and surface methicillin-resistant Staphylococcus aureus contamination

Shiomori, Teruo; Miyamoto, Hiroshi; Makishima, Kazumi; Yoshida, Minako; Fujiyoshi, Tsutsumi; Udaka, Tsuyoshi; Inaba, Tetsuya; Hiraki, Nobuaki

doi:10.1053/jhin.2001.1136

Cited by 176 publications

(159 citation statements)

References 21 publications

Supporting

Mentioning

146

Contrasting

Unclassified

Order By: Relevance

“…Although MRSA is known to be transmitted by close contact with infected individuals or by contaminated hands or clothing (Cooper et al 2004), airborne dispersal and transmission may be involved in the progression of epidemics (Shiomori et al 2002). The presence of MRSA in the nostrils of healthcare staff led Cookson et al (1989) to speculate that a possible transmission route of this bacterium is by inhalation.…”

Section: Introductionmentioning

confidence: 99%

“…The low viability of airborne S. aureus found in the ICU (1.5%) and GYM (6.2%) may be related to cleaning and disinfecting procedures implemented at these two sites. Although S. aureus has been reported be infectious at the relatively high dose of 10 5 organisms (Schmid-Hempel and Frank 2007), the viable S. aureus found in the ICU could still pose a health risk to seriously ill patients via inhalation (Shiomori et al 2002).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Optimization of Propidium Monoazide Quantitative PCR for Evaluating Performances of Bioaerosol Samplers for Sampling AirborneStaphylococcus aureus

Tseng

Hsiao

Chang

et al. 2014

Aerosol Science and Technology

View full text Add to dashboard Cite

Airborne Staphylococcus aureus causes a significant proportion of nosocomial infections. The purpose of this study was to combine real-time quantitative polymerase chain reaction with the DNA-binding agent propidium monoazide (PMA-qPCR) to assess exposures to airborne S. aureus. In this work, we generated a S. aureus aerosol and used our assay to detect viable, airborne S. aureus in a study chamber. The biological collection efficiencies of three samplers (the AGI-30 impinger, BioSampler, and Nuclepore filter sampler) were evaluated using the S. aureus aerosols. The effects of storage in collection fluid on S. aureus sampled by the AGI-30 impinger and BioSampler were evaluated. Furthermore, air samples from an intensive care unit (ICU) and a gymnasium (GYM) were subsequently used to test the performance of a BioSampler combined with our PMA-qPCR technique. The BioSampler was more effective than the AGI-30 and Nuclepore filter samplers for preserving the culturability and viability of S. aureus aerosol samples. After sampling by impingement, the loss of viable S. aureus was minimized by treating the cells with PMA prior to storage at ¡20 C and analyzing the samples by qPCR within 3 weeks. In field applications, we noted that traditional culture assays tended to underestimate the viable concentrations of S. aureus by approximately one order of magnitude. Overall, combining qPCR with and without PMA staining may be useful for assessing exposure to airborne S. aureus. However, a complex set of parameters that may affect the efficiency of PMA-qPCR must be taken into account before applying PMA-qPCR to bioaerosol detection.

show abstract

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

Optimization of Propidium Monoazide Quantitative PCR for Evaluating Performances of Bioaerosol Samplers for Sampling AirborneStaphylococcus aureus

Tseng

Hsiao

Chang

et al. 2014

Aerosol Science and Technology

View full text Add to dashboard Cite

show abstract

“…Atualmente, registra-se um aumento significativo na freqüência do isolamento de bactérias que eram reconhecidamente sensíveis aos fármacos de rotina usados na clínica, mas que se apresentam agora resistentes a quase todos os fármacos disponíveis no mercado, como ocorre com várias bactérias multirresistentes (Shiomori et al, 2002;Sakagami & Kajamura, 2006).…”

Section: Introductionunclassified

Atividades antimicrobiana e leishmanicida das sementes de Pterodon emarginatus Vogel

Dutra¹,

Braga²,

Coimbra³

et al. 2009

Rev. bras. farmacogn.

View full text Add to dashboard Cite

"Antimicrobial and leishmanicidal activities of seeds of Pterodon emarginatus". The present work investigated the antimicrobial and leishmanicidal activities of seeds of Pterodon emarginatus. The tests of diffusion in agar (10, 25 and 50 mg) and determination of minimum inhibitory concentration (MIC) were performed using essential oil (EO) obtained from seeds using the standard microorganisms: Staphylococcus aureus ATCC 25923, Streptococcus mutans ATCC 25175, Pseudomonas aeruginosa ATCC 90271, Escherichia coli ATCC 10530 and Candida albicans ATCC 10231. Leishmanicidal activity of the EO and fractions (6.25 -100 μg/ml) obtained of seeds of P. emarginatus was evaluated in vitro using L. amazonensis and L. chagasi promastigote forms. The EO inhibited the growth of S. aureus (MIC = 2.5 mg/ml). The hexane (IC 50 = 50.06 µg/ ml) and butanol (IC 50 = 46.65 µg/ml) fractions showed activity against L. amazonensis promastigote forms, but did not against L. chagasi promastigote forms. The results indicate that the bioactive molecules present in the seeds of P. emarginatus can be used as prototype for the development of drug and/or as source pharmaceutical material.

show abstract

“…Atualmente, registra-se um aumento signifi cativo na freqüência do isolamento de bactérias que eram reconhecidamente sensíveis às drogas de rotina usadas na clínica, mas que se apresentam agora resistentes a todos ou quase todos os fármacos disponíveis no mercado, como ocorre com várias bactérias multirresistentes (Huycke et al, 1998;Rattan et al, 1998;Piddock et al, 1998;Pittet, 2002;Shiomori et al, 2002;Sakagami;Kajamura, 2006).…”

Section: Introductionunclassified

Atividade antimicrobiana de óleos essenciais produzidos por Baccharis dracunculifolia D.C. e Baccharis uncinella D.C. (Asteraceae)

Ferronatto¹,

Marchesan²,

Pezenti³

et al. 2007

Rev. bras. farmacogn.

View full text Add to dashboard Cite

RESUMO:Os óleos essenciais obtidos de plantas nativas de Baccharis dracunculifolia e Baccharis uncinella, também conhecido como óleo-de-vassoura, é utilizado na indústria de perfumaria, proporcionando um aroma exótico a diversos perfumes, além de muitos estudos sobre atividades biológicas dessas espécies destacam os efeitos alelopáticos, antioxidante, antimicrobianos, citotóxicos e antiinfl amatórios. A vassoura é uma planta arbustiva de ocorrência espontânea no Brasil, assim como nos demais países do Mercosul. A composição do óleo depende da região geográfi ca e do processo de extração utilizado e a importância comercial está diretamente relacionada com a concentração de compostos oxigenados, destacando o nerolidol e o espatulenol. Óleos essenciais obtidos dessas duas espécies pelo processo de hidrodestilação, foram avaliados pelo método de difusão em disco de papel, em placas de Petri contendo meio de Müeller-Hinton, semeadas com suspensões bacterianas previamente ajustadas ao tubo 0,5 da escala de Mac Farland de quatro cepas provenientes da American Type Culture Colection: Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923 e Pseudomonas aeruginosa ATCC 27853. Discos contendo diferentes volumes dos óleos essenciais sem qualquer diluição (1, 3, 5 e 10 μL/disco) foram adicionadas às placas e incubadas a 36 o C por 24 a 48 horas. Discos de cloranfenicol (CLO) e amoxicilina (AMO) com volumes de 50 e 10 μg/disco respectivamente, foram utilizados como controle. Os resultados apresentados revelam que ambos os óleos avaliados apresentam atividade antimicrobiana sobre S. aureus, E. coli e P. aeruginosa. Unitermos:Baccharis, Asteraceae, atividade antimicrobiana, óleo essencial. ABSTRACT: "Antimicrobial activity of essential oils produced by Baccharis dracunculifolia D.C. and Baccharis uncinella D.C. (Asteraceae)". Essential oils obtained from the native plantsBaccharis dracunculifolia and Baccharis uncinella, known also as "óleo de vassoura", are used in the perfumery industry, providing diverse perfumes with exotic aromas. Besides several studies about the biological activities of these species highlight the allelopathic, antioxidant, antimicrobial, cytotoxic and antiinfl ammatory effects. The broom is a bushy plant which occurs spontaneously in Brazil as well as in the other countries from Mercosul. The oil composition depends on the geographical region and on the concentration of oxygenated blends, highlighting the nerolidol and the sphatulenol. Essential oils obtained from these two species by the hydrodistillation process were assessed by the diffusion method in paper disc, in Petri plaques containing Müeller-Hinton agar, with bacteria suspensions, previously adjusted to the tube 0,5 of Mac Farland scale of four strains which came from the American Type Culture Collection: Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923 and Pseudomonas aeruginosa ATCC 27853. Discs containing different volumes of the essential oils (1, 3, 5 and 10 (μL/disc) were added to plaques and incubated at 36 ºC for 24...

show abstract

Evaluation of bedmaking-related airborne and surface methicillin-resistant Staphylococcus aureus contamination

Cited by 176 publications

References 21 publications

Optimization of Propidium Monoazide Quantitative PCR for Evaluating Performances of Bioaerosol Samplers for Sampling AirborneStaphylococcus aureus

Optimization of Propidium Monoazide Quantitative PCR for Evaluating Performances of Bioaerosol Samplers for Sampling AirborneStaphylococcus aureus

Atividades antimicrobiana e leishmanicida das sementes de Pterodon emarginatus Vogel

Atividade antimicrobiana de óleos essenciais produzidos por Baccharis dracunculifolia D.C. e Baccharis uncinella D.C. (Asteraceae)

Contact Info

Product

Resources

About