Evaluation of Bacteriophage Anti-Biofilm Activity for Potential Control of Orthopedic Implant-Related Infections Caused by<i>Staphylococcus aureus</i>

Morris, Jodie L.; Kelly, N.; Elliott, Lisa; Grant, Alan; Wilkinson, Matthew; Hazratwala, Kaushik; McEwen, Peter

doi:10.1089/sur.2018.135

Cited by 50 publications

(57 citation statements)

References 31 publications

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“…Disease control requires bacterial biofilm disruption, as demonstrated at least in the case of human pathogens (Fong et al, 2017;Morris et al, 2019). Using CLSM analysis, we found that after 6h of incubation, Xccϕ1 disrupts the stability of Xcc biofilm (Figure 4).…”

Section: Discussionmentioning

confidence: 87%

Plant Dynamic Metabolic Response to Bacteriophage Treatment After Xanthomonas campestris pv. campestris Infection

et al. 2020

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Periodic epidemics of black rot disease occur worldwide causing substantial yield losses. Xanthomonas campestris pv. campestris (Xcc) represents one of the most common bacteria able to cause the above disease in cruciferous plants such as broccoli, cabbage, cauliflower, and Arabidopsis thaliana. In agriculture, several strategies are being developed to contain the Xanthomonas infection. The use of bacteriophages could represent a valid and efficient approach to overcome this widespread phenomenon. Several studies have highlighted the potential usefulness of implementing phage therapy to control plant diseases as well as Xcc infection. In the present study, we characterized the effect of a lytic phage on the plant Brassica oleracea var. gongylodes infected with Xcc and, for the first time, the correlated plant metabolic response. The results highlighted the potential benefits of bacteriophages: reduction of bacterium proliferation, alteration of the biofilm structure and/or modulation of the plant metabolism and defense response.

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Section: Discussionmentioning

confidence: 87%

Plant Dynamic Metabolic Response to Bacteriophage Treatment After Xanthomonas campestris pv. campestris Infection

et al. 2020

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“…Some reports have shown the efficiency of phages therapy in medical, veterinary, agricultural, and aquaculture applications without adverse safety concerns for eukaryotic cells. 13,37,38 Phages administration has shown to stimulate the host immune response, depending on the route of administration, e.g., oral and topical administrations may induce the production of anti-phages neutralizing antibodies, while intravenous administration of phages has the potential to stimulate innate and adaptive immune responses. 13,39 No significant differences were identified on the local tissue response following the implantation of phages-loaded hydrogels, sustaining their biosafety profile.…”

Section: Discussionmentioning

confidence: 99%

“…13,37,38 Phages administration has shown to stimulate the host immune response, depending on the route of administration, e.g., oral and topical administrations may induce the production of anti-phages neutralizing antibodies, while intravenous administration of phages has the potential to stimulate innate and adaptive immune responses. 13,39 No significant differences were identified on the local tissue response following the implantation of phages-loaded hydrogels, sustaining their biosafety profile. Moreover, by in vitro cytocompatibility, it was observed that phages and hydrogels' leachable products did not interfere with osteoblastic cells' viability, proliferation and morphology, indicating that the phage-loaded biomaterial systems could allow for bone cells spreading and growth.…”

Section: Discussionmentioning

confidence: 99%

“…Bacteriophages (phages) have emerged as an alternative approach to current antimicrobial chemotherapy due to their capacity to infect and kill specific bacterial strains, without modifying the established commensal microbiome. 9,10,[12][13][14][15] Phages are virulent viruses that recognize specific receptors in bacteria, injecting their genetic material and using the host biochemical machinery to replicate new phages' particles and enzymes, responsible for subsequent bacterial lysis. Furthermore, newly formed phages particles are released spreading and infecting other target bacteria nearby.…”

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confidence: 99%

“…10,12,17 Several studies have explored the potential of systemic phages administration for the treatment of implant-related infections. [13][14][15][18][19][20][21] Nonetheless, experimental studies using phages-loaded biomaterials for local delivery approaches are scarce, 9,22,23 focusing on the phages release and antimicrobial activity, without simultaneously exploring the potential combination with a regeneration inductive biomaterial. Therefore, this work aims to develop a multi-functional regenerative biomaterial for local phage delivery, based on an alginate-nanohydroxyapatite (Alg-nanoHA) hydrogel system.…”

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confidence: 99%

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Encapsulated bacteriophages in alginate-nanohydroxyapatite hydrogel as a novel delivery system to prevent orthopedic implant-associated infections

Barros

Melo

Silva

et al. 2020

Nanomedicine: Nanotechnology, Biology and Medicine

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An innovative delivery system based on bacteriophages-loaded alginate-nanohydroxyapatite hydrogel was developed as a multifunctional approach for local tissue regeneration and infection prevention and control. Bacteriophages were efficiently encapsulated, without jeopardizing phage viability and functionality, nor affecting hydrogel morphology and chemical composition. Bacteriophage delivery occurred by swelling-disintegration-degradation process of the alginate structure and was influenced by environmental pH. Good tissue response was observed following the implantation of bacteriophages-loaded hydrogels, sustaining their biosafety profile. Bacteriophagesloaded hydrogels did not affect osteoblastic cells' proliferation and morphology. A strong osteogenic and mineralization response was promoted through the implantation of hydrogels system with nanohydroxyapatite. Lastly, bacteriophages-loaded hydrogel showed excellent antimicrobial activity inhibiting the attachment and colonization of multidrug-resistant E. faecalis surrounding and within femoral tissues. This new local delivery approach could be a promising approach to prevent and control bacterial contamination during implantation and bone integration.

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Production of Antibacterial Activity and Bone Cell Proliferation by Surface Engineering of Ga‐ or Mn‐Doped Ceria‐Coated Biomedical Titanium Alloy

et al. 2022

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The present work reports a detailed interpretation of the role of Ga and Mn dopants, solid solubility mechanisms, charge compensation mechanisms, intervalence charge transfer, antibacterial performance, and cell attachment and proliferation. Sol–gel undoped and doped (1, 5, and 9 mol%) CeO2 films are spin‐coated on 3D printed Ti6Al4V biomedical alloy substrates and annealed at 650 °C for 2 h in air. Material characterization includes scanning electron microscopy (SEM), 3D scanning laser confocal microscopy, glancing angle X‐ray diffraction (GAXRD), and X‐ray photoelectron spectroscopy (XPS). In vitro testing includes inhibition of bacterial growth, simulated body fluid (SBF) testing, and cell attachment and proliferation studies. The most significant outcome is that the bioactivity of ceria derives directly from the Ce3+ concentration, which itself results from solid solubility (substitutional and interstitial) and charge compensation and redox. This challenges the common assumption of the dominance of oxygen vacancies in the performance of ceria. The antibacterial activity is dependent on the type, amount, and valence of the dopant, where opposite trends are observed for gram‐positive Staphylococcus aureus and gram‐negative Escherichia coli bacteria. All of the doped samples result in enhanced cell proliferation, although this is greatest at the lowest dopant concentration. Surface hydroxyapatite formation on the samples is achieved by soaking in SBF at 2 weeks and 1 month.

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Evaluation of Bacteriophage Anti-Biofilm Activity for Potential Control of Orthopedic Implant-Related Infections Caused byStaphylococcus aureus

Cited by 50 publications

References 31 publications

Plant Dynamic Metabolic Response to Bacteriophage Treatment After Xanthomonas campestris pv. campestris Infection

Plant Dynamic Metabolic Response to Bacteriophage Treatment After Xanthomonas campestris pv. campestris Infection

Encapsulated bacteriophages in alginate-nanohydroxyapatite hydrogel as a novel delivery system to prevent orthopedic implant-associated infections

Production of Antibacterial Activity and Bone Cell Proliferation by Surface Engineering of Ga‐ or Mn‐Doped Ceria‐Coated Biomedical Titanium Alloy

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