Evaluation of bacterial diversity in a swine manure composting system contaminated with veterinary antibiotics (VAs)

Abstract

The objective of this work was identify microorganisms present in swine e uent composting system, under the contamination by most use veterinary drugs in Brazil. The composting took place for 150 days, where was addition of 200 liters of manure (these 25 liters initially contaminated with 17 antibiotics) in 25 kg of eucalyptus wood shavings. Microorganisms were measured at times (0 until 150 days), and were identi ed in the V3-V4 regions of the 16S RNAr for Bacteria, by means of next-generation sequencing (NSG). The results showing 7 different Bacteria Phyla and, 70 Bacteria Genus identi ed (more than 1% signi cance), in total there were more than 26 phyla and 585 genera of bacteria. The genus Brucella was found during mesophilic and thermophilic phases, this genus, not yet been reported in article involving composting process. These results suggested the potential of adaptation of the bacterial community with antibiotics denoted through the antibiotics. HighlightsMore than 726.00 Phyllum and Bacterial genus-level sequences have been identi ed.Phylum Proteobacterias showed greater abundance during the composting process.Proof of adaptability microorganisms (Clostridium bacteria) in aerobic process.First identi cation of Brucella genus of bacteria in swine composting process.