Evaluation of asthma–chronic obstructive pulmonary disease overlap using a mouse model of pulmonary disease

Abstract

Background Features of asthma and chronic obstructive pulmonary disease (COPD) can coexist in the same patient, in a condition termed asthma– chronic obstructive pulmonary disease overlap (ACO). ACO is heterogeneous condition exhibiting various combinations of asthma and COPD features. No clinically acceptable experimental model of ACO has been established. We aimed to establish an animal model of ACO. Methods We generated two phenotypes of ACO by administering ovalbumin and porcine pancreatic elastase in combination, and papain. The proinflammatory cytokines and cell types in bronchoalveolar lavage fluid (BALF) were investigated, and lung function parameters were measured using the FlexiVent system. Results Greater airway inflammation was observed in the asthma and both ACO models, and emphysema was found in the COPD and both ACO models. The proportion of eosinophils in BALF was elevated in the asthma and ACO-a model. Type 2 inflammatory cytokine levels were highest in the ACO-a model, and the neutrophil gelatinase–associated lipocalin level was elevated in the asthma and ACO-a model. Of lung function parameters, compliance was greater in the COPD and ACO-b model, in which elastance was lower than in the asthma model. Airway resistance increased with the methacholine concentration in the asthma and both ACO models, but not in the control or COPD model. Conclusion We established two murine models of ACO that exhibit features of asthma and COPD. We validated the clinical relevance of the ACO models based on changes in cytokine profiles and lung function. These models will be useful in further studies of the pathogenesis of, and therapeutic targets for ACO.

Effect of budesonide formoterol combined with tiotropium bromide on pulmonary function and inflammatory factors in patients with asthma–COPD overlap syndrome

“…SOD can effectively scavenge oxygen-free radicals and resist free radical damage. 25 In the present study, improvement of the above-mentioned indexes in the experimental group was significantly better than that in the conventional group after treatment, suggesting that the combination regimen could improve endothelial functioning of patients, regulate vascular tension, and effectively protect against serum lipid peroxidation injury, consistent with similar reports in the literature. [26][27][28] Meanwhile, regarding adverse reactions, this study observed no serious adverse reactions in patients treated with combination regimen, thereby demonstrating its potential clinical safety.…”

Association of IL‐33 in modeling type‐2 airway inflammation and pulmonary emphysema in mice

“… 3 Another study demonstrated that the PPE and ovalbumin‐induced ACO model also develops allergic asthma. 4 Our overlap model used the previously reported A. alternata administration schedule. 2 This administration schedule increases the number of ILC2 and CD4 + T cells containing IL‐5 and ‐13 intracellularly in BAL fluid.…”

Correction: Evaluation of asthma–chronic obstructive pulmonary disease overlap using a mouse model of pulmonary disease

“…Correction: Evaluation of asthma-chronic obstructive pulmonary disease overlap using a mouse model of pulmonary disease Yong Suk Jo Following the publication of the original article [1], the authors identified an error in the author's name of Tai Joon An. The given name and family name were erroneously transposed.…”

“…The incorrect author's name is: An Tai Joon The correct author's name is: Tai Joon An The author group has been updated above and the original article [1] has been corrected.…”