Evaluation of Arthrospira sp. enzyme hydrolysate as dietary additive in gilthead seabream (Sparus aurata) juveniles

Galafat, Alba; Vizcaíno, Antonio Jesús; Sáez, María Isabel Corbí; Martı́nez, T.F.; Jerez-Cepa, Ismael; Mancera, Juan Miguel; Alarcón, Francisco Javier

doi:10.1007/s10811-020-02141-0

Cited by 22 publications

(37 citation statements)

References 68 publications

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“…In other studies with S. aurata juveniles, it was observed that low dietary supplementation with Arthrospira hydrolysates (2 and 4%) had no effects on the intestinal mucosa 38 . Similar results were found by Saenz et al (personal communication/unpublished results) in S. aurata within the present study.…”

Section: Discussionmentioning

confidence: 82%

“… 71 . Raw microalgae paste at provided were collected and immediately used for enzymatic hydrolysis according to a previous described protocol 38 . Briefly, N. gaditana sludge containing up to 150 g L −1 of raw microalgae biomass was hydrolyzed using commercial enzymes with cellulase activity (Viscozyme ® ) under controlled conditions (pH 5.0 and 50 °C under continuous stirring) for 4 h, providing 2% (w/w) enzyme.…”

Section: Methodsmentioning

confidence: 99%

“…For example, some microalgal species contain lectins, which are considered anti-nutritional elements 36 , 37 . However 38 , in gilthead seabream ( Sparus aurata ) juveniles fed with diets supplemented with Arthrospira enzymatic hydrolysates (2 and 4%), highlighted beneficial effects by describing increased activities of digestive enzymes such as trypsin, leucine aminopeptidase, or alkaline phosphatase, improving digestive processes and intestinal absorption capacity , and low inclusion of N. gaditana in S. aurata showed to be sufficient to obtain optimum growth without substantially increasing the cost of the feed 39 .…”

Section: Introductionmentioning

confidence: 99%

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Microbiota composition and intestinal integrity remain unaltered after the inclusion of hydrolysed Nannochloropsis gaditana in Sparus aurata diet

Cerezo

Zeo-Sánchez

García‐Márquez

et al. 2021

Sci Rep

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The use of lysed microalgae in the diet of carnivorous fish can increase the bioavailability of proteins and bioactive compounds, such as unsaturated fatty acids or vitamins in the digestive tract. These are essential molecules for the proper physiological development of fish in aquaculture. However, some antinutritional components and other undesirable molecules can be released from an excess of microalgae supplied, compromising the integrity of the intestine. The inclusion of small amounts of hydrolized microalgae in the fish diet can be a good strategy to avoid negative effects, improving the availability of beneficial compounds. Nannochloropsis gaditana is an interesting microalgae as it contains nutraceuticals. Previous studies reported beneficial effects after its inclusion in the diet of Sparus aurata, a widely cultured species in Europe and in all Mediterranean countries. However, administration of raw microalgae can produce intestinal inflammation, increased intestinal permeability, bacterial translocation and disturbance of digestion and absorption processes. The aim of this study was to evaluate changes in the intestinal microbiota and barrier stability of S. aurata fed with low inclusion (5%) hydrolysed N. gaditana. Intestinal microbiota was analyzed using Illumina MiSeq technology and libraries were constructed using variable regions V3–V4 of 16S rDNA molecules. Analysis were based in the identification, quantification and comparison of sequences. The predictive intestinal microbial functionality was analyzed with PICRUSt software. The results determined that the intestinal microbiota bacterial composition and the predictive intestinal microbiota functionality did not change statistically after the inclusion of N. gaditana on the diet. The study of gene expression showed that genes involved in intestinal permeability and integrity were not altered in fish treated with the experimental diet. The potential functionality and bacterial taxonomic composition of the intestinal microbiota, and the expression of integrity and permeability genes in the intestine of the carnivorous fish S. aurata were not affected by the inclusion of hydrolysed 5% N. gaditana microalgae.

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Section: Discussionmentioning

confidence: 82%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Microbiota composition and intestinal integrity remain unaltered after the inclusion of hydrolysed Nannochloropsis gaditana in Sparus aurata diet

Cerezo

Zeo-Sánchez

García‐Márquez

et al. 2021

Sci Rep

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“…As commonly accepted, the economic value of farmed gilthead sea bream grows directly with an increase in skin pigmentation, especially in the forefront area between the eyes [49]. Because of this, researchers have been focusing on different dietary interventions to enhance skin colour using a variety of pigment sources, such as microalgae [49][50][51][52][53], synthetic astaxanthin [51] and vegetable sources [54,55]. Despite the fact that the use of crustaceans as an active colourant has been previously investigated, the information on the use of Procambarus clarkii is limited to the only study performed on Pagrus pagrus by García et al [56].…”

Section: Discussionmentioning

confidence: 99%

Appetite Regulation, Growth Performances and Fish Quality Are Modulated by Alternative Dietary Protein Ingredients in Gilthead Sea Bream (Sparus aurata) Culture

Pulido-Rodríguez

Cardinaletti

Secci

et al. 2021

Animals

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By answering the need for increasing sustainability in aquaculture, the present study aimed to compare growth, gene expression involved in appetite regulation, physical characteristics, and chemical composition of Sparus aurata fed alternative protein sources. Fish were fed ten iso-proteic, iso-lipidic, and isoenergetic diets: a vegetable-based (CV) and a marine ingredient-rich (CF) diet were set as control diets. The others were prepared by replacing graded levels (10, 20 or 40%) of the vegetable proteins in the CV with proteins from a commercial defatted Hermetia illucens pupae meal (H), poultry by-product meal (PBM) singly (H10, H20, H40, P20, P40) or in combination (H10P30), red swamp crayfish meal (RC10) and from a blend (2:1, w:w) of Tisochrysis lutea and Tetraselmis suecica (MA10) dried biomasses. The increase in ghre gene expression observed in MA10 fed fish matched with increased feed intake and increased feed conversion ratio. Besides, the MA10 diet conferred a lighter aspect to the fish skin (p < 0.05) than the others. Overall, no detrimental effects of H, PBM, and RC meal included in the diets were observed, and fish fatty acid profile resulted as comparable among these groups and CV, thus demonstrating the possibility to introduce H, PBM, and RC in partial replacement of vegetable proteins in the diet for Sparus aurata.

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“…Among the vast variety of microalgae species, Haematococcus pluvialis stands out as a source of astaxanthin [8], a pigment included routinely in commercial feeds for salmonids. With respect to Arthrospira, this is one of Foods 2021, 10, 910 2 of 13 the most widely assessed cyanobacteria genera in aquaculture, not only owing to its high nutritional value, but also to its acknowledged antioxidant activity [9,10].…”

Section: Introductionmentioning

confidence: 99%

Assessing the Potential of Algae Extracts for Extending the Shelf Life of Rainbow Trout (Oncorhynchus mykiss) Fillets

Sáez

Suárez

Alarcón

et al. 2021

Foods

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This study evaluates the potential of different algae extracts (Crassiphycus corneus, Cc; Ulva ohnoi, Uo; Arthrospira platensis, Ap; Haematococcus pluvialis, Hp) as additives for the preservation of rainbow trout fillets. The extracts were prepared with different water to ethanol ratios from the four algae species. The highest ferric reducing antioxidant power (FRAP) was observed in Uo extracted in 80% ethanol. Ap aqueous extract also had considerable FRAP activity, in agreement with a high total phenolic content. Radical scavenging activity (DPPH) was higher in Cc 80% ethanol extract, in agreement with a high total carotenoid content. In fact, when the algae aqueous extracts were assayed on the fish fillets, their antioxidant activity exceeded that of ascorbic acid (ASC). All algae extracts delayed microbial growth and lipid oxidation processes in trout fillets throughout the cold storage period compared to controls, and also improved textural parameters, these effects being more evident for Ap and Hp. With respect to the color parameters, the Hp extract prevented the a* values (redness) from decreasing throughout cold storage, a key point when it comes to colored species, not least salmonids. On the other hand, the Ap extract was not as effective as the rest of treatments in avoiding a* and b* decrease throughout the storage period, and thereby the color parameters were impaired. The results obtained, together with the natural origin and the viability for large-scale cultivation, make algae extracts interesting fish preservative agents for the food industry.

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Evaluation of Arthrospira sp. enzyme hydrolysate as dietary additive in gilthead seabream (Sparus aurata) juveniles

Cited by 22 publications

References 68 publications

Microbiota composition and intestinal integrity remain unaltered after the inclusion of hydrolysed Nannochloropsis gaditana in Sparus aurata diet

Microbiota composition and intestinal integrity remain unaltered after the inclusion of hydrolysed Nannochloropsis gaditana in Sparus aurata diet

Appetite Regulation, Growth Performances and Fish Quality Are Modulated by Alternative Dietary Protein Ingredients in Gilthead Sea Bream (Sparus aurata) Culture

Assessing the Potential of Algae Extracts for Extending the Shelf Life of Rainbow Trout (Oncorhynchus mykiss) Fillets

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