Evaluation of antioxidant capacity with total phenolic content of Galanthus krasnovii (Amaryllidaceae)

Abstract

Natural products have gained the great interest due to their broad spectrum of biological activities. Galanthus krasnovii was dried at shade then extracted with hexane, dichloromethane, and ethyl acetate successively. After removing of solvent by reduced pressure, crude extracts of each solvent were yielded. Antioxidant activity including 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, 2,2'-azinobis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical cation and reducing power assays were carried out for each extract. Moreover, total phenolic content was determined. Total phenolic content of hexane-, dichloromethane-, and ethyl acetate extracts were found as 60.95 mg GAE/g extract, 71.90 GAE/g extract and 58.90 GAE/g extract respectively. Dichloromethane and ethyl acetate extract revealed the moderate antioxidant activity. ÖZDoğal ürünler geniş spektrumlu biyolojik aktivite göstermelerinden dolayı yoğun ilgi görmektedirler. Galanthus krasnovii gölgede kurutulduktan sonra sırasıyla hekzan, diklorometan ve etil asetat ile ekstrakte edildi. Düşük basınçta çözücü uzaklaştırıldıktan sonra her bir çözücünün ekstraktı elde edildi. Her bir ekstraktın, 1,1-difenil-2-pikralhidrazil(DPPH) radikal, 2,2'-azino-bis(3-etilbenzotiazolin-6sülfirik asit) (ABTS) radikal katyon ve indirgeme gücü antioksidan çalışmaları gerçekleştirildi. Ayrıca, ekstraktların toplam fenolik içerikleri belirlendi. Hekzan-, diklorometan-ve etil asetat ekstraktlarının toplam fenolik içerikleri sırasıyla 60.95 mg GAE/g ekstrakt, 71.90 GAE/g ekstrakt ve 58.90 GAE/g ekstrakt olarak belirlendi. Diklorometan ve etil asetat ekstraktları orta derece aktivite gösterdi. Citation:To cite this article: Erenler R, Genç N, Elmastaş M, Eminağaoğlu Ö (2019). Evaluation of antioxidant capacity with total phenolic content of Galanthus krasnovii (Amaryllidaceae). Turk J Biod 2(1): 13-17.Evaluation of antioxidant capacity with total phenolic content of Galanthus krasnovii 14 | Erenler R, Genç N, Elmastaş M, Eminağaoğlu Ö (2019). Turkish Journal of Biodiversity 2(1): 13-17

Green Synthesis of Silver Nanoparticles from Salvia aethiopis L. and Their Antioxidant Activity

“…Aromatic and medicinal plants have been used extensively for medicinal purposes for years due to their bioactive contents [36][37][38][39]. After the development of spectroscopy in the 19th century, the isolation and identi cation of secondary metabolites from corresponding plants gained great interest and it became the focus of science [33,40].…”

Biosynthesis and Biological Activities of Newly Discovered Amaryllidaceae Alkaloids

“…Several of these novel AAs belong to known structural types, while others harbor new structures. The 91 AAs were classified in this manuscript as I) norbelladine-type (1-6), II) cherylline-type (7, 8), III) galantamine-type (9-16), IV) lycorine-type (17-25), V) homolycorine-type (26)(27)(28)(29)(30), VI) crinine-type (31-50), VII) narciclasine-type (51), VIII) pretazettine-type (52-54), IX) montanine-type (55), and X) other types including AAs related to plicamine (56)(57)(58)(59)(60)(61)(62)(63)(64)(65)(66)(67), seco-plicamine (68-70), cripowellin (71)(72)(73)(74)(75)(76), mesembrine (77,78), and various others AAs (79)(80)(81)(82)(83)(84)(85)(86)(87)(88)(89)(90)(91). Structures of novel AAs belonging to the I to III scaffold types (norbelladine-, cherylline-, and galantamine-type) are depicted in Figure 3, whereas types IV and V (lycorine-and homolycorine-type) are represented in Figure 4.…”

“…Twenty species are included in the genus Galanthus native of Europe and of Middle East [91]. From whole plants of Galanthus fosteri Baker, three new AAs, namely oxoincartine (23), 3,11-O-diacetyl-9-O-demethylmaritidine (41) and 11-O-acetyl-9-O-demethylmaritidine (42) belonging to the lycorine and crinine-type, were isolated together with seven known compounds.…”

Biological Activities of Snowdrop (Galanthus spp., Family Amaryllidaceae)

“…While dichloromethane extract displayed greater reducing potential in cupric ion reducing power assay that ethanol extract. Antioxidant activity of hexane, dichloromethane and ethyl acetate extracts of G. krasnovii were investigated via DPPH and ABTS radical scavenging and cupric ion reducing power assay (Erenler et al, 2019). Dichloromethane extract demonstrated the highest ABTS activity (IC 50 14.33 μg/ml) and reducing power (1.15 µmol TE/mg).…”