Evaluation of Antioxidant and Hepatoprotective Activities  of Moringa oleifera Lam. Leaves in Carbon  Tetrachloride-Intoxicated Rats

Singh, Dharmendra Kumar; Arya, Priya Vrat; Aggarwal, Ved Prakash; Gupta, Radhey S.

doi:10.3390/antiox3030569

Cited by 69 publications

(43 citation statements)

References 34 publications

(33 reference statements)

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“…3 Traditional folklore, oral history and native materia medica have associated Moringa oleifera with a range of medicinal, nutritional and miscellaneous applications. 4 Medicinally, the antioxidant, wound healing, hypotensive, and diuretic effects of this plant have been reported. [5][6][7] Nutritionally, the plant has been reported to be rich in calcium which boosts milk production in lactating mothers.…”

Section: Introductionmentioning

confidence: 99%

Acute toxicity of the aqueous-methanolic Moringa oleifera (Lam) leaf extract on female Wistar albino rats

Okumu

Mbaria

Kanja

et al. 2016

Int J Basic Clin Pharmacol

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Background: Herbal preparations are widely assumed to be safe on oral administration and therefore the documentation of the toxic potential of some herbal concoctions used as medicine and nutrients is limited. Moringa oleifera (MO) is a plant that is gaining tremendous popularity in rural communities in Kenya as a means of offsetting nutritional and medicinal needs. However, very little is known about the safety of the plant on oral administration. Thus, the aim of the current study was to assess the biochemical and histological changes in the liver following the administration of an aqueous-methanolic (AQ-ME) MO leaf extract in female Wistar albino rats. Methods: Acute oral toxicity study on the AQ-ME MO leaf extract was conducted by the use of the limit test dose of the up and down procedure (OECD guideline number 425) with slight modifications. Briefly, ten (10) healthy, nulliparous, non-pregnant female Wistar strain albino rats aged 8-12 weeks and weighing 180±20 grams were used for the study. These animals were randomly selected into two groups; control and treatment group each having five (5) animals. They were then labelled to enable identification and control group animals were orally administered with physiological buffer saline once daily over a 48-hour period. The five (5) rats in the treatment group were dosed orally one at a time and once daily with a 2000 mg/kg dose of the AQ-ME MO leaf extract to determine the median lethal dose over a 48 hour period. Blood was then collected and used to prepare serum for biochemical analysis of aspartate amino transferase (AST), alanine amino transferase (ALT) and total bilirubin (TB) which are important biomarkers of liver dysfunction. Biochemical assays of these enzymes were performed using the method of the International Federation of Clinical Chemists (IFCC). Death was used as an endpoint, livers harvested and used to prepare transverse sections for histopathological examination. These sections were stained using the haematoxylin and eosin (H&E) method and observed for pathological changes using an optical microscope. Results: A 2000 mg/kg oral dose of AQ-ME MO leaf extract caused a significant (p<0.05) increase in the mean levels of AST but a non-significant (p>0.05) increase in the mean levels of total bilirubin in the treatment group relative to the control group. On the other hand, the extract caused a nonsignificant (p>0.05) decrease in the mean levels of ALT in the treatment group relative to the control. The post mortem analysis of the hepatic index (liver to body weight ratio) revealed that there was a non-significant increase (p>0.05) in the hepatic index of the treatment group relative to the control. However, the transverse liver sections of treatment group animals showed mild distortions in the architecture of liver cells. Conclusions: Based on these results, the LD 50 of the AQ-ME MO leaf extract was found to be >2000 mg/kg in female wistar albino rats.

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Section: Introductionmentioning

confidence: 99%

Acute toxicity of the aqueous-methanolic Moringa oleifera (Lam) leaf extract on female Wistar albino rats

Okumu

Mbaria

Kanja

et al. 2016

Int J Basic Clin Pharmacol

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“…Thus, exploring a plant for its bioactive components from different regions of the world, in search of new active principles, always holds significance. There are scanty reports on the identification of metabolites of M. oleifera from Rajasthan, which focus only on pods and in vivo antioxidant activity (Paliwal et al 2011;Mathur and Kamal 2012;Singh et al 2014). The present work was undertaken to investigate and compare the phytochemicals and antioxidant potential of plant parts (Flower, bark and leaves) of M. oleifera from Rajasthan.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of bioactive compounds and antioxidant potential of hydroethanolic extract of Moringa oleifera Lam. from Rajasthan, India

Vats

Gupta

2017

Physiol Mol Biol Plants

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Moringa oleifera Lam., the miracle tree, is widely used as a traditional medicine. The analyses of phytochemicals and antioxidant potential of hydroethanolic extract of various plant parts of M. oleifera revealed that leaves possessed the highest content of total phenolics (9.58 mg/g), b-carotene (14.10 mg/g) and lycopene (2.60 mg/g). Flowers and bark showed the highest content of total flavonoids (3.5 mg/g) and anthocyanin (52.80 mg/g), respectively. Leaves also showed maximum antioxidant potential using nitric oxide scavenging assay (IC 50 -120 lg/ml) and deoxyribose degradation assay (IC 50 -178 lg/ml). Highest DPPH radical scavenging activity was observed in flowers (IC 50 -405 lg/ml). The GC-MS study revealed the presence of 29, 36 and 24 compounds in bark, leaf and flower, respectively. The major constituent identified were epiglobulol (41.68% in bark), phytol (23.54% in leaf) and b-sitosterol (15.35% in flower).The phytochemicals identified possess several therapeutic activity, including antioxidant potential, which was confirmed through earlier reports. Moreover, the presence of 1,1,3-triethoxubutane in all the plant parts analyzed, projects it as an important source of waste water treatment as hydrophobic modifiers.

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“…The reduction in the levels of these parameters toward the respective normal values by Moringa oleifera is an indication of the stabilization of plasma membranes as well as repair of hepatic tissue damage caused by INH/RIF [40]. The administration of silymarin decreased serum AST and ALT activities as compared to the hepatotoxic rats [26].…”

Section: Discussionmentioning

confidence: 99%

Protective Effects of Moringa oleifera extract on Isoniazid and Rifampicin Induced Hepatotoxicity in Rats: Involvment of Adiponectin and Tumor Necrosis Factor-α

elhameed¹,

Salama

Attia

et al. 2018

NIDOC-ASRT

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Evaluation of Antioxidant and Hepatoprotective Activities of Moringa oleifera Lam. Leaves in Carbon Tetrachloride-Intoxicated Rats

Cited by 69 publications

References 34 publications

Acute toxicity of the aqueous-methanolic Moringa oleifera (Lam) leaf extract on female Wistar albino rats

Acute toxicity of the aqueous-methanolic Moringa oleifera (Lam) leaf extract on female Wistar albino rats

Evaluation of bioactive compounds and antioxidant potential of hydroethanolic extract of Moringa oleifera Lam. from Rajasthan, India

Protective Effects of Moringa oleifera extract on Isoniazid and Rifampicin Induced Hepatotoxicity in Rats: Involvment of Adiponectin and Tumor Necrosis Factor-α

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