Evaluation of Antioxidant and Antibacterial Activities of White Mulberry (Morus alba L.) Fruit Extracts

Suriyaprom, Sureeporn; Kaewkod, Thida; Promputtha, Itthayakorn; Desvaux, Mickaël; Tragoolpua, Yingmanee

doi:10.3390/plants10122736

Cited by 25 publications

(7 citation statements)

References 60 publications

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“…These were 0.5 mg/mL when the white mulberry extract was tested and 0.25 mg/mL for the red raspberry extract. In contrast, the methanolic extract of wild raspberry leaves from the Balkan region and aqueous mulberry extracts from Mae Hong Son were the most effective against E. coli ATCC 8739 [38,73].…”

Section: Antibacterial Activitymentioning

confidence: 84%

Antioxidant and Antibacterial Activity of Extracts from Selected Plant Material

et al. 2022

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Plants are a valuable source of biologically active molecules, mainly phenolic compounds. In the present study, the total phenolic content (TPC), DPPH· and ABTS+ scavenging activity as well as ferric reducing ability (FRAP) of aqueous ethanolic (70%) extracts of Cistus incanus L. and Asarum europaeum L. herb, Geum urbanum L. rhizome, Angelica archangelica L. root, white mulberry (Morus alba L.), lemon balm (Melisa officinalis L.), red raspberry (Rubus idaeus L.) and Betula pendula Roth. leaves were determined. In addition, the phenolic profiles of the studied plant extracts and antibacterial activity have been investigated. The extracts from C. incanus and G. urbanum demonstrated the highest TPC and antioxidant capacity, while the extracts from A. archangelica and white mulberry were characterized by the lowest values. A remarkable correlation was also found between the TPC and antioxidant activity of the examined extracts. HPLC analysis showed that the studied extracts were sources of both phenolic acids and flavonoids. More flavonoids than phenolic acids were identified in the extracts of C. incanus, M. alba, R. idaeus and B. pendula compared to the other extracts tested. Not all extracts showed a significant impact on the growth of the tested bacterial strains. Escherichia coli was the most sensitive strain to lemon balm extract (MIC, 0.125 mg/mL), whereas the strains of Acinetobacter baumannii and Bordetella bronchiseptica were sensitive to the G. urbanum extract (MIC, 0.125 mg/mL). Among Gram-positive bacteria, Enterococcus faecalis was the most sensitive to G. urbanum extract. In turn, Staphylococcus aureus and Staphylococcus epidermidis were sensitive to the extracts from C. incanus herb (MIC, 0.125 mg/mL), red raspberry (MIC, 0.125 mg/mL) and lemon balm leaves (MIC. 0.25 mg/mL). Based on the obtained results, the applicability of the studied plant extracts as additives to food and cosmetic products may be considered in the future.

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Section: Antibacterial Activitymentioning

confidence: 84%

Antioxidant and Antibacterial Activity of Extracts from Selected Plant Material

et al. 2022

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“…Subjecting the bacteria to a concentration of 0.5 MIC at 6 time points (2, 4, 8, 12, 20, and 24 h) did not reduce the CFU counts of S. aureus even after 24 h (Pang et al, 2019). The mulberry fruit extracts had certain bactericidal activity against S. aureus ATCC 25923, taking 24 h to kill the bacteria (Suriyaprom et al, 2021). However, other research has reported the separation of specific substances from mulberries that show positive results against tested pathogens by the time-kill assay.…”

Section: Antibacterial Activity By the Time-kill Kinetic Methodsmentioning

confidence: 91%

Antibacterial effect of ethanolic Morus alba Linn. leaf extract against mastitis-causing Escherichia coli and Staphylococcus aureus in vitro

Phengvongsone¹,

Thamrongyoswittayakul²,

Sukon³

et al. 2022

VIS

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This study was conducted to determine the in vitro antibacterial effect of an ethanolic Morus alba L. (mulberry) leaf extract against Escherichia coli and Staphylococcus aureus isolated from mastitis dairy cows and against E. coli ATCC 25922 and S. aureus ATCC 25923 reference strains. The antibacterial efficacy was evaluated by a microdilution method to determine the MIC, MBC, and a time-kill assay by using azithromycin as a positive control. The data were analyzed nonparametric tests with a significance level of p<0.05. The quercetin content of the mulberry leaf extract was 83.187 ± 0.272 µg/g dried plant material, as determined by HPLC. The extract had MICs of 10, 20, 10, and 10 mg/mL and MBCs of 20, 20, 20, and 10 mg/mL for E. coli ATCC 25922, E. coli ECCM62, S. aureus ATCC 25923, and S. aureus SACM62, respectively. Quercetin had relevant MIC of 10 mg/ml and MBC of 20 mg/mL to the extract for both ATCC strains. Time-kill tests showed a complete elimination of S. aureus ATCC 25923 and S. aureus SACM62 after a 30-min exposure to 10 and 20 mg/mL M. alba extract. E. coli ATCC 25922 and E. coli ECCM62 showed reductions of 6.8 log CFU/mL after a 360 min exposure to 40 mg/mL M. alba extract. In this study, the inhibitory effect was stronger against S. aureus than against E. coli (p<0.05). Overall, the M. alba L. extract showed appreciable in vitro antibacterial efficacy against mastitis-causing E. coli and S. aureus strains and against reference ATCC strains

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“…Various biological properties of different mulberry extracts have been investigated and reported including medicinal potentials such as anti-cancer activity [ 52 ], anti-bacterial activity, and antioxidant activity [ 53 ]. Importantly, their toxicity needs to be assessed concurrently for a safe application.…”

Section: Discussionmentioning

confidence: 99%

Mulberroside F from In Vitro Culture of Mulberry and the Potential Use of the Root Extracts in Cosmeceutical Applications

Thamrongwatwongsa

Pattarapipatkul

Jaithon

et al. 2022

Plants

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Mulberry (Morus spp.) is primarily used in sericulture, and its uses also extend to the food, pharmaceutical, and cosmetic industries. Mulberry extracts are rich in many bioactive compounds that exhibit a wide range of biological properties. Mulberroside F (Moracin M-6, 3′-di-O-β-D-glucopyranoside), one of the bioactive compounds found in mulberry, has previously been reported as a whitening agent by inhibiting melanin synthesis and exhibiting antioxidant effects. However, there is still limited information on the presence of this compound in plants cultured in vitro. In this study, the mulberroside F content, biochemical, and cytotoxic properties of the extracts from mulberry cultured in vitro were determined. The results revealed that both root and callus were found to be a potential source of mulberroside F. Furthermore, the mulberroside F content was positively correlated with the inhibitory effects on tyrosinase activity. Cell viability assay also revealed that crude extract of the mulberry root has no cytotoxicity in both human keratinocyte cell line (HaCaT) and Vero cells. Taken together, mulberry tissue culture represents a possible alternative and continuous production of mulberroside F, which could be further utilized in cosmeceutical applications.

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Evaluation of Antioxidant and Antibacterial Activities of White Mulberry (Morus alba L.) Fruit Extracts

Cited by 25 publications

References 60 publications

Antioxidant and Antibacterial Activity of Extracts from Selected Plant Material

Antioxidant and Antibacterial Activity of Extracts from Selected Plant Material

Antibacterial effect of ethanolic Morus alba Linn. leaf extract against mastitis-causing Escherichia coli and Staphylococcus aureus in vitro

Mulberroside F from In Vitro Culture of Mulberry and the Potential Use of the Root Extracts in Cosmeceutical Applications

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