Evaluation of antioxidant and anti-proliferative efficacy of Nostoc muscorum NCCU-442

Yasin, Durdana; Zafaryab, Md.; Ansari, Sabbir; Ahmad, Naeem; Khan, Noor Fatima; Zaki, Almaz; Rizvi, M. Moshahid Alam; Fatma, Tasneem

doi:10.1016/j.bcab.2018.12.001

Cited by 23 publications

(11 citation statements)

References 37 publications

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“…Indeed, the search for natural antioxidant compounds has gained increasing interest, considering current synthetic antioxidant compounds contain preservatives and suspected toxicity [ 10 ]. The marine environment has been a privileged niche for the research of bioactive compounds in cyanobacteria [ 11 ]. However, we can currently find in the literature a huge diversity of publications on the antioxidant activity of cyanobacteria from freshwater and terrestrial habitats [ 5 , 6 , 7 , 9 , 12 , 13 , 14 , 15 , 16 , 17 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

“…In general, these studies point to an interest in exploring the antioxidant activity of cyanobacteria, considering their richness of compounds such as carotenoids and polyphenols. Inclusively, some of these authors reported antioxidant activity [ 5 , 13 , 16 , 17 , 19 , 20 ] and/or antioxidant contents [ 11 , 14 , 15 , 21 ] in cyanobacteria quite comparable, and sometimes higher, than those found in eukaryotic microalgae, macroalgae or higher plants [ 22 , 23 , 24 , 25 ].…”

Section: Introductionmentioning

confidence: 99%

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Antioxidant and Cytoprotective Properties of Cyanobacteria: Potential for Biotechnological Applications

Guerreiro

Andrade

Menezes

et al. 2020

Toxins

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Antioxidant compounds from cyanobacteria may constitute a natural alternative to current synthetic antioxidants, which contain preservatives and suspected toxicity. In this work, we evaluate the antioxidant potential of cyanobacterial strains of distinct species/genus isolated from freshwater (n = 6), soil (n = 1) and wastewater (n = 1) environments. Lyophilized biomass obtained from in-vitro cultures of those strains was extracted with ethanol and methanol. The antioxidant potential was evaluated by chemical (DPPH scavenging method, β-carotene bleaching assay, determination of total phenolic and total flavonoid compounds) and biological (H2O2-exposed HEK293T cell line model) approach. Some strains showed high yields of antioxidant activity by the DPPH assay (up to 10.7% IP/20.7 TE μg/mL) and by the β-carotene bleaching assay (up to 828.94 AAC), as well as significant content in phenolic (123.16 mg EAG/g DW) and flavonoid (900.60 mg EQR/g DW) compounds. Normalization of data in a “per cell” or “per cell volume” base might facilitate the comparison between strains. Additionally, most of the cyanobacterial extracts conferred some degree of protection to HEK293T cells against the H2O2-induced cytotoxicity. Freshwater Aphanizomenon gracile (LMECYA 009) and Aphanizomenon flos-aquae (LMECYA 088), terrestrial Nostoc (LMECYA 291) and wastewater Planktothrix mougeotii (LEGE 06224) seem to be promising strains for further investigation on cyanobacteria antioxidant potential.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Antioxidant and Cytoprotective Properties of Cyanobacteria: Potential for Biotechnological Applications

Guerreiro

Andrade

Menezes

et al. 2020

Toxins

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“…2). Multiple sequence analysis was done by grouping other cyanobacteria like P. agardhi [34], L. aestuarii, O. cyanobacterium and N. commune with A. platensis [35,36] which has been reported to have antioxidant property. It is noted that the functional regions of AHc from all the species taken for analysis are highly conserved among them, which further proved the potentiality of the molecule.…”

Section: Apahc Homology Search and Its Phylogenymentioning

confidence: 99%

Antioxidant molecular mechanism of adenosyl homocysteinase from cyanobacteria and its wound healing process in fibroblast cells

et al. 2020

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An antioxidant molecule namely, adenosyl homocysteinase (AHc) was identified from the earlier constructed transcriptome database of Spirulina, where it was cultured in a sulphur deprived condition. From the AHc protein, a small peptide NL13 was identified using bioinformatics tools and was predicted to have antioxidant property. Further, the peptide was synthesised and its antioxidant mechanism was addressed at molecular level. NL13 was subjected to various antioxidant assays including DPPH assay, HARS assay, SARS Assay, NO assay and ABTS assay, where NL13 exhibited significant (P < 0.05) potential antioxidant activity compared to its antioxidant control, Trolox. Cytotoxicity was performed on Human whole blood and the cell viability was performed on VERO fibroblast cells. In both assays, it was found that NL13 did not exhibit any cytotoxic effect towards the cells. Further, the intracellular ROS was performed on Multimode reader followed by imaging on fluorescence microscope which showed scavenging activity even at lower concentration of NL13 (31.2 µM). An effective wound healing property of NL13 on VERO cells was confirmed by analysing the cell migration rate at two different time intervals (24 and 48 h). Overall, the study shows that NL13 peptide scavenges the intracellular oxidative stress. Keywords Cyanobacteria • Adenosyl homocysteinase • Wound healing • Fibroblast cells • Spirulina Abbreviations AHc Adenosyl homocysteinase ROS Reactive oxygen species Ap Arthrospira platensis TEAE Trolox equivalent antioxidant capacity HRS Hydroxyl radical scavenging VERO Fibroblast cells Electronic supplementary material The online version of this article (

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“…Cryptophycin isolated from cyanobacteria, such as Nostoc sp. GSV224 (a Nostoc species discovered at the University of Hawaii to be a cryptophycin producer), possess anti-cancer activity against KB human nasopharyngeal cancer cells and LoVo human colorectal cancer cells (Patterson et al, 1991;Yasin et al, 2019). The cytotoxicity induced by cryptophycins was due to its interaction with microtubule-binding proteins disrupting tubulin dynamics and eventually causing death of tumour cells (Panda, Himes, Moore, Wilson, & Jordan, 1997).…”

Section: Anti-cancer Activity Of Cyanobacteriamentioning

confidence: 99%

“…Curacin and dolastatin have been shown to possess cytotoxic activity (Gerwick et al, 2001;Jordan & Wilson, 1998;Moore et al, 1996) and have potential for anti-cancer drug development (Chaganty, Golakoti, Heltzel, Moore, & Yoshida, 2004;Moore et al, 1996). Curacin-A was first isolated from Lyngbya majuscula (Gerwick, Roberts, Proteau, & Chen, 1994) and acts as an inhibitor of cell growth and mitosis activity by interacting with tubulin (Verdier-Pinard et al, 1998;Xiong, O'Keefe, Byrd, & McMahon, 2006;Yasin et al, 2019). Nostoc sp.…”

Section: Anti-cancer Activity Of Cyanobacteriamentioning

confidence: 99%

Cyanotherapeutics: an emerging field for future drug discovery

Tiwari

2020

Applied Phycology

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Cyanobacteria are ubiquitously distributed oxygenic photosynthetic prokaryotes, present even in extreme environments. Many cyanobacteria have been found to be excellent sources of proteins and bioactive compounds. Their cosmopolitan availability, abundant important bioactive compounds and their associated health benefits make cyanobacteria an ideal target for therapeutic applications. Cyanobacteria have a potential role in nutraceutics and are also important in drug development. Several studies have advocated the use of cyanobacteria and their active principles in the treatment of various diseases because cyanobacteria display anti-cancer, antibacterial, antiimmunosuppressive, anti-tumour, neuroprotective, and antiviral activities. Thus, in this review, we have summarized and updated the therapeutic applications of cyanobacteria in drug development, with the introduction of the new term "cyanotherapeutics".

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Evaluation of antioxidant and anti-proliferative efficacy of Nostoc muscorum NCCU-442

Cited by 23 publications

References 37 publications

Antioxidant and Cytoprotective Properties of Cyanobacteria: Potential for Biotechnological Applications

Antioxidant and Cytoprotective Properties of Cyanobacteria: Potential for Biotechnological Applications

Antioxidant molecular mechanism of adenosyl homocysteinase from cyanobacteria and its wound healing process in fibroblast cells

Cyanotherapeutics: an emerging field for future drug discovery

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