Evaluation of antimicrobial properties of bovine lactoferrin against foodborne pathogenic microorganisms in planktonic and biofilm forms (in vitro)

Shahidi, Fakhri; Roshanak, Sahar; Javadmanesh, Ali; Yazdi, Farideh Tabatabaei; Pirkhezranian, Zana; Azghandi, Marjan

doi:10.1007/s00003-020-01280-3

Cited by 13 publications

(7 citation statements)

References 34 publications

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“…It is broadly distributed in specific neutrophil granules of polymorphonuclear leukocytes as well as biological fluids, such as milk, saliva, and seminal fluid [ 87 ]. Lf displays a broad range of preventive, therapeutic, and biological activities, including antifungal, antibacterial, antiviral, anticancer, antioxidant, immunomodulatory, cell-growth modulatory, and binding functions, in addition to neutralizing some bioactive substances, such as lipopolysaccharide (LPS) and glycosaminoglycan [ [88] , [89] , [90] , [91] , [92] ]. Additionally, Lf is a member of the transferrin family and has a higher affinity to bind to iron than transferrin [ 93 ].…”

Section: Lactoferrinmentioning

confidence: 99%

The role of iron in the pathogenesis of COVID-19 and possible treatment with lactoferrin and other iron chelators

Habib

Ibrahim²,

Zaim³

et al. 2021

Biomedicine & Pharmacotherapy

203

189

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Section: Lactoferrinmentioning

confidence: 99%

The role of iron in the pathogenesis of COVID-19 and possible treatment with lactoferrin and other iron chelators

Habib

Ibrahim²,

Zaim³

et al. 2021

Biomedicine & Pharmacotherapy

203

189

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“…The McFarland standard 0.5 was used as standard to measure the turbidity. Medium without inoculum was considered as negative control and Gentamicin (μg/mL) used as positive control 32 . After MIC test, 100 μL of dilutions with no bacterial growth pour-plated on Mueller–Hinton agar medium and incubated at 37 °C for 24 h. The MBC was determined based on the lowest bactericidal concentration showing no visible growth on culture plates 32 , 33 .…”

Section: Methodsmentioning

confidence: 99%

Antibacterial effects assessment on some livestock pathogens, thermal stability and proposing a probable reason for different levels of activity of thanatin

Javadmanesh

Mohammadi

Mousavi

et al. 2021

Sci Rep

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There is a continuing need to prevent the increasing use of common antibiotic and find the replacement to combat the drug/antibiotic resistant bacteria such as antimicrobial peptides (AMPs) such as thanatin peptide. In this study, recombinant thanatin peptide was expressed in the HEK293 cell line. Then the antimicrobial properties of this peptide on some poultry and farm animal’s pathogen strains were assessed. The thermal-stability of thanatin was predicted in various temperatures through in silico analysis. Afterwards, according to Minimum Inhibitory Concentration (MIC) results, Escherichia coli and Pseudomonas aeruginosa were chosen to test the hypothesis of LptA/LptD–thanatin interaction, computationally. Relative amino acid sequences and crystallography structures were retrieved and missed tertiary structures were predicted. The interaction of thanatin with LptA and LptD of Escherichia coli and Pseudomonas aeruginosa were analyzed subsequently. The antibacterial activity of thanatin peptide was evaluated between 6.25 and 100 μg/mL using minimum inhibitory concentration. Also, the amounts of minimum bactericidal concentrations (MBC) were between 12.5 and 200 μg/mL. The bioinformatics analysis followed by the in vitro assessment, demonstrated that thanatin would be thermally stable in the body temperature of poultry and farm animals. Thanatin could penetrate to the outer membrane domain of LptD in Escherichia coli and it could block the transition path of this protein while the entrance of LptD in Pseudomonas aeruginosa was blocked for thanatin by extra residues in comparison with Escherichia coli LptD. In addition, the quality of interaction, with regard to the number and distance of interactions which leads to higher binding energy for thanatin and LptD of Escherichia coli was much better than Pseudomonas aeruginosa. But the site and quality of interaction for thanatin and LptA was almost the same for Escherichia coli and Pseudomonas aeruginosa. Accordingly, thanatin can prevent the assembly of LptA periplasmic bridge in both pathogens. The antibacterial and thermal stability of the thanatin peptide suggested that thanatin peptide might serve as a natural alternative instead of common antibiotics in the veterinary medicine. The outcome of this in silico study supports the MIC results. Therefore, a probable reason for different level of activity of thanatin against Escherichia coli and Pseudomonas aeruginosa might be the quality of LptA/LptD–thanatin interaction.

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“…Lactoferrin has been found to have an anti-inflammatory effect on humans [ 19 ]. Furthermore, Lf chelates iron (Fe), rendering the ionized form of Fe ineffective against invading microbes [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

“…The novel severe acute respiratory syndrome coronavirus (SARS-CoV-2), the causative agent of coronavirus disease 2019 (COVID- 19), was first discovered in Wuhan, China in December 2019. It causes a viral pneumonia which may progress to acute respiratory distress and death [1].…”

Section: Introductionmentioning

confidence: 99%

The Prospect of Lactoferrin Use as Adjunctive Agent in Management of SARS-CoV-2 Patients: A Randomized Pilot Study

et al. 2021

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Background and Objectives: Preventive, adjunctive and curative properties of lactoferrin have been evaluated since the first wave of severe acute respiratory syndrome coronavirus (SARS-CoV), a viral respiratory disease, emerged 18 years ago. Despite the discovery of new vaccine candidates, there is currently no widely approved treatment for SARS-CoV-2 (COVID-19). Strict adherence to infection prevention and control procedures, as well as vaccines, can, however, prevent the spread of SARS-CoV-2. This study aimed to evaluate the efficacy of lactoferrin treatment in improving clinical symptoms and laboratory indices among individuals with mild to moderate coronavirus disease-19 (COVID-19). Materials and Method: A randomized, prospective, interventional pilot study conducted between 8 July and 18 September 2020 used a hospital-based sample of 54 laboratory-confirmed participants with mild to moderate symptoms of COVID-19. Randomization into a control and two treatment groups ensured all groups received the approved Egyptian COVID-19 management protocol; only treatment group participants received lactoferrin at different doses for seven days. Clinical symptoms and laboratory indices were assessed on Days 0, 2 and 7 after starting treatments. Mean values with standard deviation and one-way analysis of variance with least significant difference of demographic and laboratory data between control and treatment groups were calculated. Results: Our study showed no statistically significant difference among studied groups regarding recovery of symptoms or laboratory improvement. Conclusions: Further research into therapeutic properties particularly related to dosage, duration and follow-up after treatment with lactoferrin in individuals with COVID-19 is required.

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Evaluation of antimicrobial properties of bovine lactoferrin against foodborne pathogenic microorganisms in planktonic and biofilm forms (in vitro)

Cited by 13 publications

References 34 publications

The role of iron in the pathogenesis of COVID-19 and possible treatment with lactoferrin and other iron chelators

The role of iron in the pathogenesis of COVID-19 and possible treatment with lactoferrin and other iron chelators

Antibacterial effects assessment on some livestock pathogens, thermal stability and proposing a probable reason for different levels of activity of thanatin

The Prospect of Lactoferrin Use as Adjunctive Agent in Management of SARS-CoV-2 Patients: A Randomized Pilot Study

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