Evaluation of antimicrobial activity of propolis and nanopropolis against Staphylococcus aureus and Candida albicans

Afrouzan, Houshang; Amirinia, Cyrus; Mirhadi, Sayed Ahmad; Ebadollahi, Alireza; Vaseji, Narges; Tahmasbi, Golamhosein

doi:10.5897/ajmr11.1183

Cited by 10 publications

(3 citation statements)

References 27 publications

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“…Furthermore, nanoscale systems have been utilized to enhance the biological and physical properties of antimicrobial agents used in endodontics thereby improving their effectiveness in root canal debridement 3 . In this context, previous nano-herbal irrigants showed promising results regarding antibacterial and anti-biofilm activity in vitro [19][20][21][22][23] . Therefore, this study assessed the effect of 20mg/ml propolis nanoparticles extract solution versus 2.5% NaOCl as root canal irrigant on the intensity of postoperative pain, the amount of bacterial reduction, and analgesic intake following single visit root canal treatment of asymptomatic mandibular premolars with necrotic pulps in a prospective, randomized, double-blinded, parallel clinical trial.…”

Section: Discussionmentioning

confidence: 97%

Effect of Propolis nanoparticles versus sodium hypochlorite as root canal irrigant on postoperative pain and bacterial reduction in mandibular premolars with necrotic pulps: a randomized clinical trial

Elaguizy,

Emara,

Dwedar

et al. 2024

Egyptian Dental Journal

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Aim:The aim of this study was to clinically compare the intensity of post-operative pain and the amount of bacterial load reduction after using 20mg/ml propolis nanoparticles extract solution as root canal irrigant versus 2.5% NaOCl during chemo-mechanical preparation in asymptomatic necrotic mandibular premolars treated in a single visit.Methodology: This is a double blinded, parallel, prospective randomized controlled trial, with allocation ratio 1:1. Fifty patients having single canalled necrotic mandibular premolar were included. Intervention group received 20 mg/ml propolis nanoparticles solution as irrigant, while control group used 2.5% NaOCl. Single visit endodontic treatment was done using EdgeEndo files. Postoperative pain was measured at 6, 12, 24, and 48 hours using Numerical Rating Scale. Culturing was used to assess intracanal bacterial levels before and after preparation. Also, the number of analgesic tablets were counted. All demographic, baseline and outcome data were statistically analyzed. A P-value < 0.05 was considered statistically significant.Results: Postoperative pain in both groups was similar regarding incidence and intensity (P>0.05). No significant difference in the incidence or the number of analgesic tablets taken by the patients was detected in both groups (P>0.05). Both irrigants significantly reduced the intracanal bacterial load however, propolis nanoparticles solution showed superior efficacy against anaerobes (P = 0.027). Conclusion:Propolis nanoparticles solution was similar to NaOCl regarding incidence and intensity of postoperative pain but showed higher anaerobic bacterial reduction when treating asymptomatic necrotic mandibular premolars in a single visit.

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Section: Discussionmentioning

confidence: 97%

Effect of Propolis nanoparticles versus sodium hypochlorite as root canal irrigant on postoperative pain and bacterial reduction in mandibular premolars with necrotic pulps: a randomized clinical trial

Elaguizy,

Emara,

Dwedar

et al. 2024

Egyptian Dental Journal

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“…Their nanometer-size could promote effective permeation through cell membranes and stability in the bloodstream. Alginate is a natural polymer found in all species of brown algae (Afrouzan et al, 2012). ALg NPs are well known for their controlled drug release properties and used for the encapsulation of many active pharmaceutical agents (El-Houssiny et al, 2016.…”

Section: Introductionmentioning

confidence: 99%

Egyptian Propolis 13: Influence of Propolis and Alginate Propolis NPs on Egyptian-Nubian Goats Serum Immunoglobulins and Cytokines Level

Hegazı¹,

El-Houssiny²,

Sadek³

et al. 2020

AAVS

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This investigation aimed to improve and enhance the immune system status of newborn Egyptian-Nubian goats using Alginate nanoparticles (ALg NPs) as a new drug carrier for the oral delivery of propolis. Propolis was selected as a natural additive of colostrum due to its amazing functional properties. In addition, through its implementation into ALg NPs, its handling properties have been improved and potentiated. Alginate-propolis NPs were prepared by the controlled gellification method. Morphological analysis of the ALg-propolis NPs was examined using a Transmission Electron Microscope (TEM). The flavonoids content of the propolis was analyzed by HPLC. Thirty twins Egyptian Nubian goats (Zaraibi) kids were randomly allotted into three groups; 10 in each group. The rearing systems during the suckling period were extended to 13 weeks as follows: C: Free suckling (FS), where the born kids were kept with their dams until being 13 weeks old (control). T1: (FS) + 0.6 ml propolis (twice/week). T2: (FS) + 0.06 ml Alg-propolis NPs (twice/week). The kids were weighed biweekly and the daily body weight gains were recorded. The serum levels of immunoglobulins; IgA and IgG, serum total protein as well as the serum cytokine levels; IFN-γ, TNFα, IL1β, and IL6 after treatment with propolis and ALg-Propolis NPs were determined at different treatment time. The HPLC analysis revealed 15 flavonoid compounds that are characteristic of propolis. The TEM result showed that the ALg-Propolis NPs are discrete and have spherical shapes with small particle sizes in the nanometer scale (10 nm). Also, the results revealed that both propolis and ALg-Propolis NPs caused a significant increase (P<0.05) of the serum IgG and IgA immunoglobulin levels and a decrease of the serum cytokine levels (IFN-γ, TNFα, IL1β, and IL6) of newborn Egyptian-Nubian goats. However, the ALg-Propolis NPs have a more potent effect on the IgG and IgA immunoglobulin levels and cytokine levels than pure propolis. The results indicated that the nano-encapsulation of propolis within ALg NPs reflected on the health status of the kids, increased the titer of the immunoglobulins; IgG and IgA and reduced the pro-inflammatory cytokines. It could conclude that the feasibility of developing a successful propolis oral delivery nano-system on an industrial scale using the ALg NPs to improve the immune status of the Egyptian-Nubian newborn kids.

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“…Nanopropolis is a natural nano-material can be useful to medicine [18] and powerful antioxidant [19]. Moreover, it has been reported that the propolis in nanoform is more effective than propolis in terms of antibacterial and antifungal activity [20].…”

Section: Introductionmentioning

confidence: 99%

Cytotoxic Effect of Propolis Nanoparticles on Ehrlich Ascites Carcinoma Bearing Mice

Abdo¹,

Alsharif²,

Salah³

et al. 2019

ANP

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Background and objective Previous studies have demonstrated the anti-cancer effects of propolis. However, its use is limited because of its poor bioavailability. In the present study, the major objective was to improve propolis bioavailability using a nanosuspension formulation. The cytotoxic effect of propolis nanosuspension (PRO-NS) on the Ehrlich ascites carcinoma (EAC) in female Swiss albino mice was investigated in comparison to the free propolis. Materials and methods A propolis-loaded nanosuspension was formulated by applying solvent-antisolvent nano-precipitation technique. The prepared PRO-NS was characterized for average particle size, polydispersity index (PDI) and zeta potential. Also, the morphology of the nanosuspension particles was investigated using scanning electron microscopy (SEM). Moreover, PRO-NS cytotoxicity was tested using EAC bearing mice. The anticancer activity of Pro-NS was assessed by studying tumor volume, life span, viable and non-viable cell count, antioxidant, biochemical estimations and proliferation of EAC cells. Results The results revealed that propolis nanoparticles were relatively spherical in shape with rough surface. The tumor bearing mice treated with PRO-NS showed increased life span and inhibited tumor growth and the proliferation of EAC cells in comparison to the free propolis (p < 0.01). Moreover, Pro-NS ameliorated the increase in serum aspartate transaminase (AST) and alanine transaminase (ALT) activities, IgM and the level of creatinine and urea after implantation of EAC cells. In addition, PRO-NS improved the SOD activity and glutathione content of liver and EAC cells. Furthermore, PRO-NS inhibited the formation of lipid peroxidation products (MDA) and total IgG in EAC tumor bearing mice. Conclusions Our results indicate that PRO-NS has a strong inhibitory activity against growth of tumors in comparison to free propolis. The anti-tumor mechanism may be mediated by preventing oxidative damage, immune-stimulation and induction of apoptosis.

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Evaluation of antimicrobial activity of propolis and nanopropolis against Staphylococcus aureus and Candida albicans

Cited by 10 publications

References 27 publications

Effect of Propolis nanoparticles versus sodium hypochlorite as root canal irrigant on postoperative pain and bacterial reduction in mandibular premolars with necrotic pulps: a randomized clinical trial

Effect of Propolis nanoparticles versus sodium hypochlorite as root canal irrigant on postoperative pain and bacterial reduction in mandibular premolars with necrotic pulps: a randomized clinical trial

Egyptian Propolis 13: Influence of Propolis and Alginate Propolis NPs on Egyptian-Nubian Goats Serum Immunoglobulins and Cytokines Level

Cytotoxic Effect of Propolis Nanoparticles on Ehrlich Ascites Carcinoma Bearing Mice

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