Evaluation of Antimicrobial Activity of Bauhinia purpurea Leaves Under In Vitro Conditions

Abstract

This study was undertaken with an objective of testing the antibacterial and antifungal activities of Bauhinia purpurea leaves and identifying the bioactive compounds. The antimicrobial activity of leaf extract was determined in aqueous and organic extracts and the minimum inhibitory concentration (MIC) against six species of pathogenic and non-pathogenic microorganisms: Bacillus subtilis, Staphylococcus aureus, Salmonella typhi, Escherichia coli, Pseudomonas aeruginosa and Candida albicans using the disk diffusion method. The chemical constituents of organic plant extract were separated by thin layer chromatography and purified by column chromatography and further identified by gas chromatography-mass spectrometry (GC-MS) analysis. Significant inhibitory activity was observed with methanol extracts of plant against the test microorganisms while less antibacterial activity was observed in hexane, acetone and aqueous extracts. MIC of B. purpurea extract was B1,500 lg/ml against S. aureus and B. subtilis while this extract showed no inhibition against Gram-negative S. typhi, E. coli and P. aeruginosa or against fungus C. albicans. Eleven compounds were identified in B. purpurea leaf extract by GC-MS analysis. The composition of B. purpurea revealed the presence of lupeol, stigmasterol, lanosterol, ergosterol, beta-tocopherol, phytol, hexadeconic acids, hexadeconic acids methyl esters, octadecadienoic acids and octadecatrienoic acid. Stigmasterol and lupeol were the most abundant (34.48 and 15.63 %). Other phytosterols like lanosterol (4.15 %) and ergosterol (2.82 %) were also found to be present in this extract.

Membrane Biosynthesis Gene Disruption in Methicillin-Resistant Staphylococcus aureus (MRSA) as Potential Mechanism for Reducing Antibiotic Resistance

“…In this way it differs from other antibiotics produced by a tunicate, the Styela clava which produces styelin and clavanin, that are active against both Gram-positive and Gram-negative bacteria [14]. The synergism activities between substances extracted from natural organisms may contribute to antimicrobial ability but the mechanisms of action are complicated to predict [21].…”

Cadmium and lead remediation using magnetic and non-magnetic sustainable biosorbents derived from Bauhinia purpurea pods

“…Bauhinia purpurea is also found in Myanmar, Sri Lanka and Southern China. 34 Bauhinia purpurea leaves, owers, ower buds, and young pods are edible 35 and have medicinal value, exhibiting antioxidant, 36 anti-cancer, 37 anti-microbial, 38 and nephroprotective 39 activities. Bauhinia purpurea can withstand aqueous pollutants.…”

Hepatoprotective Activity of Methanolic Extract ofBauhinia purpureaLeaves against Paracetamol-Induced Hepatic Damage in Rats

“…For example, the plant exhibited antimicrobial [8], antinociceptive, anti-inflammatory, and antipyretic [3, 9], antimycobacterial, antimalarial, antifungal, cytotoxic, and anti-inflammatory [10], anti-nephrotoxicity [11], and wound healing [12] activities. In vitro study has demonstrated that B. purpurea possesses antiproliferative [13], antioxidant [13, 14], and antimicrobial activities [14, 15], and also has potential as hepatocellular carcinoma inhibitor [16]. Interestingly, other studies have proved that B. purpurea leaf possesses antiulcer activity [17, 18].…”