Hepatoprotective Activity of Methanolic Extract ofBauhinia purpureaLeaves against Paracetamol-Induced Hepatic Damage in Rats

Abstract

In an attempt to further establish the pharmacological properties of Bauhinia purpurea (Fabaceae), hepatoprotective potential of methanol extract of B. purpurea leaves (MEBP) was investigated using the paracetamol- (PCM-) induced liver toxicity in rats. Five groups of rats (n = 6) were used and administered orally once daily with 10% DMSO (negative control), 200 mg/kg silymarin (positive control), or MEBP (50, 250, and 500 mg/kg) for 7 days, followed by the hepatotoxicity induction using paracetamol (PCM). The blood samples and livers were collected and subjected to biochemical and microscopical analysis. The extract was also subjected to antioxidant study using the 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay with the total phenolic content (TPC) also determined. From the histological observation, lymphocyte infiltration and marked necrosis were observed in PCM-treated groups (negative control), whereas maintenance of the normal hepatic structural was observed in group pretreated with silymarin and MEBP. Hepatotoxic rats pretreated with silymarin or MEBP exhibited significant decrease (P < 0.05) in ALT and AST enzyme level. Moreover, the extract also exhibited antioxidant activity and contained high TPC. In conclusion, MEBP exerts potential hepatoprotective activity that could be partly attributed to its antioxidant activity and high phenolic content and thus warrants further investigation.

Amelioration of Paracetamol-Induced Hepatotoxicity in Rat by the Administration of Methanol Extract ofMuntingia calaburaL. Leaves

“…The PCM-induced toxicity model is commonly used to study the potential hepatoprotective activity of extracts/compounds [16, 27]. In the present investigation, the 3 g/kg of PCM which is a toxic dose, has resulted in the increment of body weight, liver weight, and LW/BW ratios of rats and showed significant elevation of serum level of hepatic enzymes ALT, AST, and ALP in comparison to normal control group, as expected.…”

Redox Control of Antioxidant and Antihepatotoxic Activities of Cassia surattensis Seed Extract against Paracetamol Intoxication in Mice: In Vitro and In Vivo Studies of Herbal Green Antioxidant

“…The oral administration of paracetamol was performed 3 hours after the last seed extract/silymarin administration on the 7th day except for the normal control group, which received only 10% DMSO. All mice were euthanized after 48 hours after paracetamol-induced hepatotoxicity [34]. …”

The protective effect of pomegranate juice in paracetamol-induced acute hepatotoxicity in rats

“…The severity of each finding was scored as 0 (normal), +1 (mild), 2++ (moderate) +++ 3 (severe) according to the study of Yahya et al (23). Afterwards, the total scores of the groups were compared.…”