Evaluation of Antimicrobial Activity of Some Compounds Isolated from Rhamnus cathartica L

Objectives: Over the past few decades, phenolic compounds become important due to it has been associated with protection against different diseases and sensory point of vision. Hence, at the present study, there has been a growing interest to carry out structural elucidation and characterization of the pure isolates from Syzygium jambos.Methods: S. jambos dried powder leaves were extracted by soaking in 85% methanol solvent at room temperature 25±2°C. The antioxidative activity of the isolates was assessed according to 2,2'-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging and phosphomolybdenum assays.Results: A total of 8 compounds were isolated from the n-butanol extract of S. jambos (Family Myrtaceae) plant, they were identified as quercetin-3-O-rutinoside (1), prenylbenzoic acid 4-β-D-glucoside (2), morolic acid 3-O-caffeate (3), 5,4'-dihydroxy, 7-methoxy, 6-methyl-flavone (4), 3,4,5-trihydroxybenzoic acid (5), quercetin (6), isoetin-7-O-β-D-glucopyranoside (7), and (4'-hydroxy-3'-methoxyphenol-β-D-[6-O-(4"-hydroxy-3",5"-dimethoxylbenzoate)] glucopyranoside) (8). Compounds 5 and 6 showed the most radical scavengers among the tested compounds with SC 50 values of 5.50 and 4.30 µg/ml, respectively, compared to ascorbic acid as standard and the total antioxidant capacity (TAC) values of 605.0 and 680.59 mg ascorbic acid equivalent/g compound, respectively. In vitro antimicrobial activities of the isolated compounds were tested using disc agar plate method against four pathogenic microbial strains including Gram-positive, Gram-negative bacteria and yeast with inhibition zones from 9 to 19 mm. Gas chromatographymass spectrometry analysis for the essential oil provides twenty four identified components representing 92% of its total oil composition. Conclusion:The results supported that S. jambos could be attributed to sources of natural antioxidant and antimicrobial applications.

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“…To evaluate the antimicrobial activity, disc agar plate method was used according to the reported method [16].…”

Section: Antimicrobial Activitymentioning

confidence: 99%

“…(Table 5). Previous reported on the antimicrobial activity of major groups from plants, for example, simple phenols, polyphenols, phenolic acids, quinones, flavonoids, flavones, flavonols, coumarins, tannins, terpenoids, and alkaloids [16,33].…”

Section: In Vitro Antioxidant Activities Of the Isolated Compoundsmentioning

confidence: 99%

Extraction, Isolation, and Characterization of Bioactive Compounds and Essential Oil From Syzygium Jambos

Ghareeb

Hamed

Abdel-aleem

et al. 2017

Asian J Pharm Clin Res

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“…Sub fraction [6][7][8][9][10][11][12][13][14] 3 :MeOH with increasing contents of methanol afforded compound 8 (45 mg) and 9 (32 mg). Fraction E was subjected to CC over silica gel using gradient elution with C 6 H 6 :EtOAc (10:0-3:7) afforded 24 subfraction (each 15 ml) possess mixture of compounds; subfraction [2][3][4][5][6][7][8][9] subjected to a preparative silica gel chromatography afforded compound 3 (27 mg), then subfractions [10][11][12][13][14][15][16][17][18][19][20][21][22][23][24] was re-purified on Sephadex LH-20 CC and eluted with MeOH:H 2 O (9.5:0.5) affording compound 10 (36 mg).…”

Section: Extraction and Isolationmentioning

confidence: 99%

Assessing the Bioactivity and Antioxidative Properties of Some Compounds Isolated From Abutilon Hirtum (Lam.)

Hamed¹,

Refahy²,

Abdel‐Aziz³

2017

Asian J Pharm Clin Res

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Objectives: The present study on the methanol extract of Abutilon hirtum (Lam.) [Malvaceae] afforded ten compounds. Findings from this assessment indicated that A. hirtum leaves possessed vast potential as medicinal product especially in liver cancer treatment.Methods: Dried-powdered leaves were boiled under reflux in 10 L of petroleum ether for 8 hrs. After filtration, the solvent was evaporated; afforded 15 g of petroleum ether extract followed by boiling with reflux for 8 hrs with chloroform, then filtration and the residue was evaporated to give 34 g chloroform extract. Ethyl acetate was added and refluxed for 8 hrs, then filtration and evaporation to give 31 g ethyl acetate extract. Finally the leaves were refluxed with 10 L of 85 % aqueous MeOH, after cooling, the solution was filtered and evaporated and the residue was 210 g methanol extract then the residue was dissolved in de-ionized water (250 ml), then the salt was removed by adding excess methanol solution (2.5 L), and finally filtered. The cytotoxicity of the isolated compounds was evaluated towards HepG2 liver-carcinoma cell line using MTT assay, the antimicrobial activity was tested using the Disc agar plate method and the total antioxidant capacity was determined according to phosphomolybdenum method. Results:The isolated compounds identified as methyl gallate, cuneataside E, bergapten, gallic acid, ellagic acid, epigallocatechin-3-O-gallate, kaempferol-3-O-α-L-rhamnoside, benzyl-1-O-β-D-glucopyranoside, 2(4-hydroxyphenyl)ethyl-O-β-D-glucopyranoside and β-sitosterol. All the isolated compounds are known; but they were isolated from Abutilon hirtum for the first time. Conclusion:This report may serve as a footprint concerning the biological and pharmacological activities of A. hirtum leaves.

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“…For instance, Bounatirou et al, (2007) described that the main components in studied plant extract that showed the antimicrobial activity were terpene phenols 19 . Moreover, as the study of Hamed et al, (2015), two flavonol compounds exhibited significant antimicrobial effect against Candida albicans, Staphylococcus aureus and Escherichia coli bacteria 20 . However, this activity may be due to the content of phorbol ester.…”

Section: Antimicrobial Activity Of the Crude Methanolic Extractmentioning

confidence: 99%

Antimicrobial Activity and Chemical Constituents of the Extract from Jatropha curcas Fruit

Saosoong¹,

Ruangviriyachai²

2015

Orient. J. Chem

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The antimicrobial activity and chemical constituents of the methanolic extract from J. curcas fruit were evaluated in this study. The crude extract was achieved by extraction with 60 % (v/v) methanol. It showed the potencies of antimicrobial activity against P. putida, P. syringae pv. sesami, X. campestris, X. campestris pv. glycines, X. campestris pv. vesicatoria and R. solanacearum with the presence of inhibition zone in the range of 8.0 ± 0.0 to 13.7 ± 0.6 mm and MIC value at 214.29 ± 0.00 µg/mL. Furthermore, flavone compound can be proposed by the analysis of gas chromatographymass spectrometry (GC-MS). According to the group of flavonoid compounds have strong bioactive properties; the results suggested that J. curcas fruit has highly potential as effective natural bioactive sources.

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Evaluation of Antimicrobial Activity of Some Compounds Isolated from Rhamnus cathartica L

Cited by 14 publications

References 0 publications

Extraction, Isolation, and Characterization of Bioactive Compounds and Essential Oil From Syzygium Jambos

Extraction, Isolation, and Characterization of Bioactive Compounds and Essential Oil From Syzygium Jambos

Assessing the Bioactivity and Antioxidative Properties of Some Compounds Isolated From Abutilon Hirtum (Lam.)

Antimicrobial Activity and Chemical Constituents of the Extract from Jatropha curcas Fruit

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