EVALUATION OF ANTIMICROBIAL ACTIVITY IN OLIVE (<I>OLEA EUROPAEA</I>) LEAF EXTRACT

Abstract

In this study the antimicrobial activity of Olive Leaf Extracts (OLE) was screened against five grampositive and five gram-negative bacteria. The antimicrobial activity was evaluated using disc diffusion and microdilution methods. The diameters of inhibition zones of the olive leaf extract were observed 13.33±2.08 mm against S. typhimurium and 21.67±1.53 mm against B. cereus. There was a similarity between the inhibition zones of olive leaf extract and Gentamicin discs against S. typhimurium, P. vulgaris and P. aeruginosa. While Minimum Inhibitory Concentrations (MIC) of olive leaves extract against, L. monocytogenes, E. coli O157, E. sakazakii and P. aeruginosa was ≥32 mg mL −1 , the MIC against the other bacteria (B. cereus, S. aureus, E. faecalis, P. vulgaris, E. coli, S. typhimurium) that used in this study was ≥16 mg mL −1 . It's considered that some other works should be conducted about using olive leaf extract in food industry as a natural antimicrobial food additivie as well as medicine and pharmaceutical industry.

A comparative study on chemical composition, antibiofilm and biological activities of leaves extracts of four Tunisian olive cultivars

“…(2003) also reported that olive leaves have antimicrobial activity against E. coli, S. aureus, B. cereus and S. typhi . The results reported in the present study show similarity with previous ones (Markin et al., 2003; Pereira et al., 2007; Sudjana et al., 2009; Lee and Lee, 2010; Gökmen et al., 2014) about the antimicrobial activity of olive leaves extracts. According to Pereira et al.…”

Antimicrobial effect of Hydroxytyrosol, Hydroxytyrosol Acetate and Hydroxytyrosol Oleate on Staphylococcus Aureus and Staphylococcus Epidermidis

“…In a study by M. Gökmen et al (25) in 2015, the MIC value of olive leaves against Listeria monocytogenes, E. coli, and Pseudomonas aeruginosa was more than 32 mg/ml. This value was higher than 16 mg/ml for Bacillus cereus and S. aureus and Enterococcus faecalis and some other bacteria.…”

High Performance Liquid Chromatography Analysis and Anti-Methicillin Resistant Staphylococcus Aureus Activity of Olive Fruit Ethanolic Extract

“…Thus, the OME was highly active (≥ 15 mm) against MRSA3, at all the extracts concentrations (1.875 -6.25 mg/well), and against all the test MRSA isolates at concentration 6.25 mg/well, while the extract (OME) was moderately active (ZDI: 12 -13 mm) against MRSA2 (at 1.875 -3.125 mg/well of OME) and against MRSA1 with 3.125 mg/well of OME; herein, the OSE had less to moderate activity against the MRSA isolates tested, based upon the previously mentioned criteria 11 . Gokmen et al 32 demonstrated the growth inhibition property of olive leaf extract against S. aureus having ZDI value of 19 mm.…”