Evaluation of antimicrobial activity and total phenolic content of three Pinus species

Abstract

This study compared the antimicrobial activity and total phenolic content of three Pinus plants (Pinus densiflora, P. thunbergii, P. rigida) for the first time. The antimicrobial activity of the water fraction of methanol extract of fresh leaves was stronger than that of fallen leaves at any concentrations. The water fraction of crude methanol extract from fresh leaves of P. thunbergii showed a higher growth inhibitory activity against gram-positive and gram-negative bacteria than that of P. densiflora and P. rigida. The results from the disc diffusion method followed by measurements of minimal inhibition concentration (MIC) indicate that Bacillus subtilis was the most sensitive microorganism with the lowest MIC value. The highest total phenolic content was found in fresh leaves of P. rigida and P. thunbergii. The assay showed that the fresh leaves of the three Pinus plants contained higher total phenolic content than fallen leaves of the three plants. The antimicrobial activity was related with the total phenolic content.

Biological activities and chemical characterization of the Lebanese endemic plant Origanum ehrenbergii Boiss

“…The antibacterial activity of the Cyclo and Dcm extracts can also be attributed to some phenolic compounds such as pinosylvin monomethyl ether present in these extracts (Table 2). Kim et al 8 showed a good antibacterial activity of pinosylvin monomethyl ether against both Gram positive and Gram negative foodborne bacteria (MIC = 200 μg/mL). Moghrovyan et al 24 tested the antibacterial activity of the ethanolic extract of O vulgare L. against E coli VKPM M‐17, E coli DH5α‐pUC1, S aureus WDCM 5233, and Salmonella enterica .…”

“…Dried oregano is used as a medication for gastrointestinal troubles, antitussive, expectorant, 7 as well as in food preparations, liquor production, and as a pesticide 2 . Today, there is a huge consumer's demand for foods that are minimally processed and free from synthetic chemical preservatives 8 …”

Screening of Wood/Forest and Vine By-Products as Sources of New Drugs for Sustainable Strategies to Control Fusarium graminearum and the Production of Mycotoxins

“…Several hypotheses can be raised to explain the activities of the pine sawdust 175 °C extract. Numerous studies have shown positive relations between antifungal efficiency of natural extracts, contents in total phenolics and antioxidant potential, i.e., free radical scavenging capacity [ 33 , 34 ]. In addition, several authors have suggested that the efficiency of phenolic acids in reducing TCTB production could be linked to their antioxidant properties [ 7 , 35 , 36 ].…”