Evaluation of antigens from various Leishmania species in a Western blot for diagnosis of American tegumentary leishmaniasis.

Gonçalves, Cely Cristina Martins; Reiche, Edna Maria Vissoci; Filho, Benício Alves de Abreu; Silveira, Thaís Gomes Verzignassi; Felizardo, Tania C.; Maia, Karoline Rocha; Costacurta, Rafael; Padovesi, Evandro José; Filho, Benedito Prado Dias; Jankevicius, Shiduca Itow; Jankevicius, José Vítor

doi:10.4269/ajtmh.2002.66.91

Cited by 27 publications

(19 citation statements)

References 52 publications

(67 reference statements)

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“…The Leishmania species analyzed shared bands of 26.6, 48.5, 50.6, 55, 62, and 72.5 kDa, supporting the close relationship between species of the subgenus Viannia, and indicating the presence of common epitopes, consistent with previous findings (Chiller et al, 1990;Cuba Cuba et al, 2001;Gonçalves et al, 2002). When comparing the frequencies of recognition between WHO and INPA leishmania antigens, notable differences were found in the number of detected bands and the frequencies with which they appear when using the antisera from Leishmania spp.-infected patients.…”

Section: Discussionsupporting

confidence: 88%

Differences in the Antigenic Profile and Infectivity of Murine Macrophages of Leishmania (Viannia) Parasites

Matta¹,

Cysne-Finkelstein²,

Machado³

et al. 2010

Journal of Parasitology

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The antigenic profile and infectivity were compared between 3 recent Leishmania (Viannia) isolates from the Amazonian region (Instituto Nacional de Pesquisas da Amazonia [INPA] strains) and 3 World Health Organization (WHO) reference species (Leishmania guyanensis, Leishmania braziliensis, and Leishmania naiffi). Differences were observed in the peak and extent of promastigote growth. The WHO reference strains exhibited significantly higher exponential growth as promastigotes than INPA strains. In the immunoblot analyses, the INPA strains revealed several specific peptide fragments, as well as the greatest recognition frequencies by sera from Leishmania sp.-infected patients; among the latter, antigens derived from L. naiffi were the most frequently recognized. In vitro infection was carried out using mice peritoneal macrophages; all strains were able to enter the macrophages, but only L. amazonensis was able to reproduce. A striking observation was that L. naiffi exhibited the longest survival time inside the macrophages. Our data strongly suggest the application of recently isolated parasites as sources of antigen for diagnosis procedures. Moreover, L. naiffi species possesses several characteristics relevant for its use as a source of novel antigens to be explored in the design of diagnostic tools and vaccines.

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Section: Discussionsupporting

confidence: 88%

Differences in the Antigenic Profile and Infectivity of Murine Macrophages of Leishmania (Viannia) Parasites

Matta¹,

Cysne-Finkelstein²,

Machado³

et al. 2010

Journal of Parasitology

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“…17,[26][27][28] The bands recognized by the sera of patients with Chagas disease and American CL, mucocutaneous leishmaniasis, and VL using homologous and heterologous antigens have been addressed previously, with different results. 17,20,[29][30][31][32] In our study, individual bands from the standard pattern were recognized by sera from patients with VL or CL, but the general pattern of reactivity of these sera was very different from the pattern of sera from Chagasic patients; in no case did it include more than four bands of those bands included in the standard pattern for Chagas disease.…”

Section: Discussioncontrasting

confidence: 57%

Identification of a Western Blot Pattern for the Specific Diagnosis of Trypanosoma cruzi Infection in Human Sera

Riera

Vergés²,

Iniesta³

et al. 2012

The American Journal of Tropical Medicine and Hygiene

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Abstract. A Western blot (WB) method using a lysate from Trypanosoma cruzi (Maracay strain) epimastigotes was evaluated. Serum samples from 37 patients with confirmed Chagas disease (cohort I), 27 Spanish patients with visceral leishmaniasis caused by Leishmania infantum (cohort II), and 28 Colombian patients with cutaneous leishmaniasis caused by L. panamensis and negative serology for Chagas disease (cohort III) were tested. The negative controls were 55 healthy seronegative subjects for T. cruzi and Leishmania; 28 of the negative controls were from a region endemic for Chagas disease and Leishmania (cohort IV), and 27 of the negative controls were from a non-endemic area for Leishmania and T. cruzi (cohort V). A homogeneous standard band pattern consisting of six antigenic bands corresponding to 28, 32, 38, 39, 40, and 48 kDa was recognized simultaneously for all Chagasic patients' sera. Sera from Leishmania-infected patients showed a heterogeneous band pattern that was easily differentiated from the pattern of patients with Chagas disease. WB with T. cruzi epimastigote antigen is an efficient method for diagnosis and may be used as an alternative to confirm T. cruzi and detect cross-reactivity with Leishmania.

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“…Goncalves et al (13) reported that bands between 13 kDa and 150 kDa were detected when they used different Leishmania species as the antigen sources. Most of the polypeptide fractions (93%) were determined in the serum samples (42).…”

Section: Discussionmentioning

confidence: 99%

“…However, several papers which described encouraging results by the use of alternative diagnostic methods have been reported from the New World (13,16,29,32).…”

mentioning

confidence: 99%

Serodiagnosis of Anthroponotic Cutaneous Leishmaniasis (ACL) Caused byLeishmania tropicain Sanliurfa Province, Turkey, Where ACL Is Highly Endemic

Zeyrek

Korkmaz

Özbel

2007

Clin Vaccine Immunol

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In this study, we aimed to evaluate the validity of the conventional enzyme-linked immunosorbent assay (ELISA) and the Western blotting test for the diagnosis of anthroponotic cutaneous leishmaniasis (ACL) using serum samples obtained from 51 patients with parasitologically proven nontreated CL (NonT-CL patients) and 62 patients under treatment for CL (UT-CL patients). Additionally, 29 serum samples obtained from patients with parasitologically and serologically proven visceral leishmaniasis (VL) were also used as positive controls, and serum samples from 43 blood donors were used as negative controls. All sera were diluted to the same dilution (1/100). Leishmania infantum MON-1 was used as the antigen in the conventional ELISA. The sera of 27 (93.1%) of 29 VL patients were seropositive by ELISA, while the sera of 40 (78.4%) of 51 NonT-CL patients and 43 (69.3%) of 62 UT-CL patients were seropositive by the conventional ELISA. The absorbance values of the CL patients' sera were significantly lower than the absorbance values of the VL patients' sera. Bands between 15 and 118 kDa were detected in two groups of CL patients. Among all bands, the 63-kDa band was found to be more sensitive (88.5%). When we evaluated the Western blotting results for the presence of at least one of the diagnostic antigenic bands, the sensitivity was calculated to be 99.1%. By using serological tests, a measurable antibody response was detected in most of the CL patients in Sanliurfa, Turkey. It is also noted that this response can be changed according to the sizes, types, and numbers of lesions that the patient has. The Western blot test was found to be more sensitive and valid than the conventional ELISA for the serodiagnosis of ACL. In some instances, when it is very difficult to demonstrate the presence of parasites in the smears, immunodiagnosis can be a valuable alternative for the diagnosis of ACL.

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Evaluation of antigens from various Leishmania species in a Western blot for diagnosis of American tegumentary leishmaniasis.

Cited by 27 publications

References 52 publications

Differences in the Antigenic Profile and Infectivity of Murine Macrophages of Leishmania (Viannia) Parasites

Differences in the Antigenic Profile and Infectivity of Murine Macrophages of Leishmania (Viannia) Parasites

Identification of a Western Blot Pattern for the Specific Diagnosis of Trypanosoma cruzi Infection in Human Sera

Serodiagnosis of Anthroponotic Cutaneous Leishmaniasis (ACL) Caused byLeishmania tropicain Sanliurfa Province, Turkey, Where ACL Is Highly Endemic

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