Evaluation of antigen-coating procedures of enzyme-linked immunosorbent assay method for detection of trypanosomal antibodies

Development and Comparison of Enzyme Immunoassays for Diagnosis of Chagas' Disease Using Fixed Forms of Trypanosoma cruzi (Epimastigotes, Amastigotes, and Trypomastigotes) and Assessment of Antigen Stability for the Three Assays

“…Compared with the freshly coated wells, there was also less variation in the precoated wells stored at either 4°C (coefficient of variation [CV] for positives, 21.5 versus 9.8; CV for negatives, 87.0 versus 23.6) or room temperature (CV for positives, 14.6 versus 8; CV for negatives, 88.7 versus 19.4). Similar assay stability has recently been reported for up to 12 months and at temperatures up to 50°C by Rebeski et al in a whole, fixed Trypanosoma congolense EIA (29,30).…”

Isolation of two antigens from Trypanosoma evansi that are partially responsible for its cross-reactivity with Trypanosoma vivax

“…In particular PCR, which is a very sensitive technique for detecting trypanosomes (Moser et al, 1989;Masiga et al, 1992;Majiwa et al, 1994;Solano and Amsler-Delafosse, 1995;Reifenberg et al, 1997;Lefrançois et al, 1998), has limited use in routine diagnosis due to the need of specific laboratory facilities, common DNA cross-contamination, and high costs. While detection of trypanosome antigens lacks sensitivity and specificity (Desquesnes, 1996;Eisler et al, 1998;Rebeski et al, 1999), assays for detection of anti-trypanosome circulating antibodies have high measures of validity and are economical and applicable at a large scale (Reyna-Bello et al, 1998;Rebeski et al, 2000;Lejon et al, 2003).…”

Field evaluation of four novel enzyme immunoassays for Chagas’ disease in Venezuela blood banks: comparison of assays using fixed‐epimastigotes, fixed‐trypomastigotes or trypomastigote excreted–secreted antigens from two Trypanosoma cruzi strains

“…no concentration steps needed). Furthermore, our previous work (Berrizbeitia et al , 2004) as well as that of Rebeski et al (2000) suggest that the fixed‐organism antigens can also be stable over prolonged periods at room temperature if stored with an appropriate desiccant. Although there were subtle differences between the performance of the fixed‐epimastigote and fixed‐trypomastigote EIAs, it is not clear whether or not the slight specificity advantage of the trypomastigote‐based test is clinically relevant or trumps the simpler protocol for growing epimastigotes in culture.…”