Evaluation of antidiabetic and antioxidant activity of Moringa oleifera in experimental diabetes

Abstract

Background: Moringa oleifera, a widely cultivated species in India, is an exceptionally nutritious vegetable with a variety of potential uses in treating rheumatism, venomous bites, and microbial infections. In the present study, we investigated the antidiabetic and antioxidant effects of methanol extracts of M. oleifera pods (MOMtE) in streptozotocin (STZ)-induced diabetic albino rats. Methods: Diabetic rats were treated with 150 or 300 mg ⁄ kg MOMtE for 21 days and the antidiabetic effects of the extract were evaluated by measuring changes in biochemical parameters in the serum and pancreatic tissue. Two phytoconstituents, namely quercetin and kaempferol, were isolated from the MOMtE extract and their structures were determined using nuclear magnetic resonance and infrared spectroscopy. Results: The progression of diabetes was significantly reduced after MOMtE treatment. In treated rats, both doses of MOMtE induced a significant reduction in serum glucose and nitric oxide, with concomitant increases in serum insulin and protein levels. Furthermore, MOMtE treatment increased antioxidant levels in pancreatic tissue, with concomitant decreases in levels of thiobarbituric acid-reactive substances. Histologic examination of the pancreas from diabetic rats showed degenerative changes in b-cells; MOMtE treatment significantly reversed the histoarchitectural damage to the islets cells. Conclusion: In conclusion, M. oleifera exerts protective effects against STZinduced diabetes. The MOMtE exhibited significant antidiabetic and antioxidant activity and active constituents may be isolated from the extract for evaluation in future clinical studies.

Chemical and Biological Aspects of Extracts from Medicinal Plants with Antidiabetic Effects

“…Treatment with the extract decreased the damage caused by oxidative stress, and may be effective in preventing protein oxidative damage, explaining the increase in protein levels in the treated animals, a mechanism involved in β-cell cytotoxicity in diabetes. Histological examination of the pancreas from diabetic rats showed degenerative changes in β-cells; MOMtE treatment significantly reversed the histoarchitectural damage to islet cells [50].…”

“…An extract of A. tricolor at different doses (50,100,200, or 400 mg/kg) administered one hour prior to glucose administration in the oral glucose tolerance test (OGTT) (p.o. ), showed a significant antihyperglycemic activity in glucose-loaded mice at all doses of the extract tested.…”

A Review on Kigelia Africana

“…However, since the synthesis of many phyto chemicals can be unbelievably difficult, [21] it is recommended that this plant be included as protected species in communities where they are found, and plantations of this plant be established as immediate source of the identified active ingredients for the clinical trials. healing, antioxidant and many more.…”

“…Like many other African food [20] and medicinal plants, [21][22][23][24] use of Kigelia africana to manage diabetes is traditionally practiced and reported. [2][3] The anti-diabetic activity and the antioxidant effect were studied.…”

Pengaruh Ekstrak Etanol Daun Kelor (Moringa oleifera) pada Ekspresi Insulin dan Insulitis Tikus Diabetes Melitus

“…Ekstrak metanol polong Moringa oleifera juga terbukti mengandung quercetin dan kaempherol yang diperkirakan meningkatkan induksi sekresi insulin oleh glukosa pada sel β pankreas yang masih berfungsi. 15 Penelitian dengan ekstrak metanol polong Moringa oleifera tersebut tidak menindaklanjuti pemeriksaan ekspresi insulin pada sel β pankreas dan insulitis pada pulau Langerhans.…”