Evaluation of antibiotic activity produced by  Pseudomonas aeruginosa LV strain against  Xanthomonas arboricola pv. pruni

Vasconcellos, Fernanda Corrêa da Silva; Oliveira, Admilton Gonçalves de; Lopes-Santos, Lucilene; Beranger, amile Priscila de Oliveira; Cely, Martha Viviana Torres; Simionato, Ane Stéfano; Pistori, Juliana F.; Spago, Flávia Regina; Mello, João Carlos Palazzo de; Martin, Juca Abramo Barrera San; Andrade, Célia Guadalupe Tardeli de Jesus; Andrade, Galdino

doi:10.4236/as.2014.51008

Cited by 17 publications

(7 citation statements)

References 15 publications

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“…In this way, the substantial control of Xcc 306 observed in a preventive treatment was likely related to mechanisms other than antibiotic activity such as SAR. The preventive effect was also observed in other studies with different species of Xanthomonas and host plants ( Lopes et al, 2012 ; Spago et al, 2014 ; Vasconcellos et al, 2014 ). Phenazine can influence growth and induce SAR in plants ( Pierson and Pierson, 2010 ), and is produced by many bacterial species.…”

Section: Discussionsupporting

confidence: 82%

Bioactive Organocopper Compound from Pseudomonas aeruginosa Inhibits the Growth of Xanthomonas citri subsp. citri

et al. 2016

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Citrus canker is a very destructive disease of citrus species. The challenge is to find new compounds that show strong antibiotic activity and low toxicity to plants and the environment. The objectives of the present study were (1) to extract, purify and evaluate the secondary metabolites with antibiotic activity produced by Pseudomonas aeruginosa LV strain in vitro against Xanthomonas citri subsp. citri (strain 306), (2) to determine the potential of semi-purified secondary metabolites in foliar application to control citrus canker under greenhouse conditions, and (3) to identify antibiotic activity in orange leaf mesophyll infected with strain 306, by electron microscopy. Two pure bioactive compounds were isolated, an organocopper antibiotic compound (OAC) and phenazine-1-carboxamide. Phenazine-1-carboxamide did not show any antibiotic activity under the experimental conditions used in this study. The OAC showed a high level of antibiotic activity with a minimum inhibitory concentration of 0.12 μg mL-1. In greenhouse tests for control of citrus canker in orange trees, the semi-purified fraction F3d reduced lesion formation by about 97%. The concentration used was 500 times lower than that for the recommended commercial copper-based product. Electron microscopy showed that F3d altered the exopolysaccharide matrix and caused cell lysis of the pathogen inside the citrus canker lesions. These results suggest that secondary metabolites produced by inducing P. aeruginosa LV strain have a high potential to be used as a bioproduct to control citrus canker.

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Section: Discussionsupporting

confidence: 82%

Bioactive Organocopper Compound from Pseudomonas aeruginosa Inhibits the Growth of Xanthomonas citri subsp. citri

et al. 2016

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“…In the previous studies was testing the effect of secondary metabolite produced by Pseudomonas aeruginosa strain LV on different phytopathogenic bacteria [8]- [10], and other pathogenic bacteria [18] [19]. In the present study was evaluate the effect secondary metabolites produced by Pseudomonas sp.…”

Section: Discussionmentioning

confidence: 98%

“…In addition, there is an increasing concern about the accumulation of such substances in food and in the environment [6]. For these reasons, the use of antagonist bacteria or their metabolites has been proposed as a promising strategy for plant protection [7]- [10].…”

Section: Introductionmentioning

confidence: 99%

Activity of Secondary Bacterial Metabolites in the Control of Citrus Canker

Murate¹,

Oliveira²,

Higashi³

et al. 2015

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This study investigated the protective effects of secondary bacterial metabolites, produced by Pseudomonas sp. (bacterium strain LN), on citrus canker disease caused by Xanthomonas axonopodis pv. citri (Xac 306). The LN bacteria strain was cultured in liquid medium and the supernatant free-cells was treated with methanol (AMF) and ethyl acetate (AEF), respectively, and then the extract was concentrated, filtrated, lyophilized and fractionated by vacuum liquid chromatography (VLC). After VLC, eight fractions were obtained. All fractions' activity against Xac 306 by agar well diffusion assay and minimum inhibitory concentration but in different concentrations were tested. Cytotoxicity effects were observed in all fractions in 50 µg•mL −1 concentration. The comet assay demonstrated that the fractions EAF, VLC2 and VLC3 presented no genotoxic effects at tested concentrations. In plants only VLC3 showed significant results (p < 0.05), reducing the incidence of citrus canker lesions.

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“…Increasing limitation of chemical treatments has stimulated interest into the application of biocontrol agents against bacterial diseases. The use of bacterial antagonists against Xap , such as strains of Pseudomonas fluorescens (Biondi et al ., ), Pseudomonas aeruginosa (da Silva Vasconcellos et al ., ) and Xanthomonas campestris (Kawaguchi et al ., ), has been experimentally assayed. Selected bacteriophages have also been considered as candidates for the control of Xap .…”

Section: Bacterial Spot Of Stone Fruits and Almond: Disease Managementmentioning

confidence: 97%

Xanthomonas arboricola pv. pruni, causal agent of bacterial spot of stone fruits and almond: its genomic and phenotypic characteristics in the X. arboricola species context

Garita-Cambronero

Palacio-Bielsa

Cubero

2018

Molecular Plant Pathology

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Management of bacterial spot of stone fruits and almond is based on an integrated approach that comprises essential measures to avoid Xap introduction in a production zone, as well as the use of tolerant or resistant plant material and chemical treatments, mainly based on copper compounds. Management programmes also include the use of appropriate cultivation practices when the disease is already established. Finally, for the effective control of the disease, appropriate detection and characterization methods are needed for use in symptomatic or asymptomatic samples as a first approach for pathogen exclusion. USEFUL WEBSITES: https://gd.eppo.int/taxon/XANTPR; http://www.cost.eu/COST_Actions/ca/CA16107; http://www.xanthomonas.org.

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Evaluation of antibiotic activity produced by Pseudomonas aeruginosa LV strain against Xanthomonas arboricola pv. pruni

Cited by 17 publications

References 15 publications

Bioactive Organocopper Compound from Pseudomonas aeruginosa Inhibits the Growth of Xanthomonas citri subsp. citri

Bioactive Organocopper Compound from Pseudomonas aeruginosa Inhibits the Growth of Xanthomonas citri subsp. citri

Activity of Secondary Bacterial Metabolites in the Control of Citrus Canker

Xanthomonas arboricola pv. pruni, causal agent of bacterial spot of stone fruits and almond: its genomic and phenotypic characteristics in the X. arboricola species context

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