Evaluation of Antibacterial Activity of Macroalgae Extracts as Adjunctive Therapy in Neonates Sepsis Induced by Klebsiella pneumoniae

Khalil, Maha A.; Ismail, Mona M.; Shafay, Shimaa M. El

doi:10.1007/s13369-020-04602-7

Cited by 5 publications

(5 citation statements)

References 26 publications

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“…Multidrug resistance (MDR) is a persistent global issue. A considerable need for revolutionary nanomaterials has developed for the construction of new antimicrobial biomaterials, which has unavoidably opened up new therapeutic horizons in medical approaches ( Khalil M. A. et al, 2015 ; El-Zawawy and Ali, 2016 ; Khalil et al, 2020 , 2021 ). To the best of our knowledge, no studies have been published on the efficacy of Bio-AgNPs produced by the marine actinobacterium Nocardiopsis dassonvillei as an antimicrobial candidate against MDR pathogens with concurrent antioxidant, insecticidal, and anticancer potential.…”

Section: Introductionmentioning

confidence: 99%

Biosynthesis of Silver Nanoparticles by Marine Actinobacterium Nocardiopsis dassonvillei and Exploring Their Therapeutic Potentials

et al. 2022

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Nanoparticles have recently emerged as a popular research topic. Because of their potential applications in therapeutic applications, biosynthesized silver nanoparticles (Bio-AgNPs) have gained much attention in recent years. Cell-free extracts (CFE) from a marine culture of actinobacteria and silver nitrate were used to reduce Ag+ ions and create Bio-AgNPs. Nocardiopsis dasonvillei KY772427, a new silver-tolerant actinomycete strain, was isolated from marine water and used to synthesize AgNPs. In order to characterize Bio-AgNPs, UV-Vis spectral analysis, Fourier transform infrared (FTIR), transmission electron microscopy (TEM), and dynamic light scattering spectroscopy (DLS) were all utilized. Using UV–Vis spectroscopy, a peak in the surface plasmon resonance (SPR) spectrum at 430 nm revealed the presence of Bio-AgNPs. The TEM revealed spherical AgNPs with a diameter of 29.28 nm. DLS determined that Bio-AgNPs have a diameter of 56.1 nm and a negative surface charge (−1.46 mV). The minimum inhibitory concentration (MIC) of Bio-AgNPs was determined against microbial strains. Using resazurin-based microtiter dilution, the synergistic effect of Bio-AgNPs with antimicrobials was investigated. Pseudomonas aeruginosa had the lowest MIC of Bio-AgNPs (4 μg/ml). Surprisingly, the combination of antimicrobials and Bio-AgNPs had a significant synergistic effect on the tested strains. The insecticidal activity of Bio-AgNPs (200 μg/ml) against Macrosiphum rosae was found to be maximal after 36 h. Additionally, Bio-AgNPs demonstrated significant scavenging activity against 2,2′-diphenyl-1-picrylhydrazyl (DPPH) and hydroxyl (OH–) radicals, with IC50 values of 4.08 and 8.9 g/ml, respectively. In vitro studies using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay revealed a concentration-dependent decrease in cell viability when CaCo2 cells were exposed to Bio-AgNPs. With the decrease in cell viability, lactate dehydrogenase leakage (LDH) increased. The findings of this study open up a new avenue for the use of marine Nocardiopsis dasonvillei to produce Bio-AgNPs, which have significant antimicrobial, antioxidant, insecticidal, and anticancer potential.

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Section: Introductionmentioning

confidence: 99%

Biosynthesis of Silver Nanoparticles by Marine Actinobacterium Nocardiopsis dassonvillei and Exploring Their Therapeutic Potentials

et al. 2022

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“…The capacity of P. aeruginosa to utilize high levels of inherent and acquired resistance mechanisms to resist most antibiotics has made eradication increasingly challenging (Salomoni et al, 2017). Hence, there is an increasing demand for the development of novel alternatives to combat MDR pathogens (Khalil et al, 2015a(Khalil et al, , 2020. Under this scope, several metallic nanomaterials have superior antibacterial activity against MDR bacteria over traditional antibiotics.…”

Section: Introductionmentioning

confidence: 99%

Enhanced Efficacy of Some Antibiotics in Presence of Silver Nanoparticles Against Multidrug Resistant Pseudomonas aeruginosa Recovered From Burn Wound Infections

et al. 2021

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Burn wound infections with multidrug-resistant (MDR) bacteria are shown in many countries as severe widespread health threats. Consequently, attention has been devoted to new nanoparticle-based materials in the field of antimicrobial chemotherapy for burn wound infections. This study aimed to evaluate both in vitro and in vivo efficacies of nanoparticle–antibiotic combinations as new classes of materials subjected against MDR Pseudomonas aeruginosa. Out of 40 Gram-negative isolates, 23 P. aeruginosa were recovered from patients with burn wound infections attending different hospitals in Tanta, Egypt. The susceptibility test revealed that 95.7% of P. aeruginosa isolates were MDR with a high incidence of resistance against carbenicillin. Antibacterial activities of silver nanoparticles (Ag-NPs) against the isolates examined showed various inhibition zone diameters ranging from 11 to 17 mm. Strong synergistic efficacy of neomycin was reported in combination with Ag-NPs against MDR P. aeruginosa P8 and P14 isolates. The in vivo effectiveness of various pharmaceutical formulations prepared from a combination of neomycin antibiotic with Ag-NPs in the treatment of induced bacterially infected mice burns showed that maximum healing activity along with faster wound contraction reported with the combination of neomycin-Ag-NPs in the spray formulation. Generally, data indicated that incorporating Ag-NPs in combination with certain antibiotics may be a new, promising application for wound treatments, especially burns infected with MDR P. aeruginosa.

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“…While SNPs’ antibacterial mechanisms of action have been widely studied and disputed, they are still not completely understood. SNPs have two well-established antibacterial mechanisms: direct and ion-mediated degradation [ 80 ]. When bacteria are exposed to SNPs, the nanoparticles bind to the cell wall’s surface [ 81 ].…”

Section: Resultsmentioning

confidence: 99%

Streptomyces rochei MS-37 as a Novel Marine Actinobacterium for Green Biosynthesis of Silver Nanoparticles and Their Biomedical Applications

et al. 2022

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Periodontitis, as one of the most common diseases on a global scale, is a public health concern. Microbial resistance to currently available antimicrobial agents is becoming a growing issue in periodontal treatment. As a result, it is critical to develop effective and environmentally friendly biomedical approaches to overcome such challenges. The investigation of Streptomyces rochei MS-37’s performance may be the first of its kind as a novel marine actinobacterium for the green biosynthesis of silver nanoparticles (SNPs) and potentials as antibacterial, anti-inflammatory, antibiofilm, and antioxidant candidates suppressing membrane-associated dental infections. Streptomyces rochei MS-37, a new marine actinobacterial strain, was used in this study for the biosynthesis of silver nanoparticles for various biomedical applications. Surface plasmon resonance spectroscopy showed a peak at 429 nm for the SNPs. The SNPs were spherical, tiny (average 23.2 nm by TEM, 59.4 nm by DLS), very stable (−26 mV), and contained capping agents. The minimum inhibitory concentrations of the SNPs that showed potential antibacterial action ranged from 8 to 128 µg/mL. Periodontal pathogens were used to perform qualitative evaluations of microbial adhesion and bacterial penetration through guided tissue regeneration membranes. The findings suggested that the presence of the SNPs could aid in the suppression of membrane-associated infection. Furthermore, when the anti-inflammatory action of the SNPs was tested using nitric oxide radical scavenging capacity and protein denaturation inhibition, it was discovered that the SNPs were extremely efficient at scavenging nitric oxide free radicals and had a strong anti-denaturation impact. The SNPs were found to be more cytotoxic to CAL27 than to human peripheral blood mononuclear cells (PBMCs), with IC50 values of 81.16 µg/mL in PBMCs and 34.03 µg/mL in CAL27. This study’s findings open a new avenue for using marine actinobacteria for silver nanoparticle biosynthesis, which holds great promise for a variety of biomedical applications, in particular periodontal treatment.

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Evaluation of Antibacterial Activity of Macroalgae Extracts as Adjunctive Therapy in Neonates Sepsis Induced by Klebsiella pneumoniae

Cited by 5 publications

References 26 publications

Biosynthesis of Silver Nanoparticles by Marine Actinobacterium Nocardiopsis dassonvillei and Exploring Their Therapeutic Potentials

Biosynthesis of Silver Nanoparticles by Marine Actinobacterium Nocardiopsis dassonvillei and Exploring Their Therapeutic Potentials

Enhanced Efficacy of Some Antibiotics in Presence of Silver Nanoparticles Against Multidrug Resistant Pseudomonas aeruginosa Recovered From Burn Wound Infections

Streptomyces rochei MS-37 as a Novel Marine Actinobacterium for Green Biosynthesis of Silver Nanoparticles and Their Biomedical Applications

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