Enhanced Efficacy of Some Antibiotics in Presence of Silver Nanoparticles Against Multidrug Resistant Pseudomonas aeruginosa Recovered From Burn Wound Infections

Abstract

Burn wound infections with multidrug-resistant (MDR) bacteria are shown in many countries as severe widespread health threats. Consequently, attention has been devoted to new nanoparticle-based materials in the field of antimicrobial chemotherapy for burn wound infections. This study aimed to evaluate both in vitro and in vivo efficacies of nanoparticle–antibiotic combinations as new classes of materials subjected against MDR Pseudomonas aeruginosa. Out of 40 Gram-negative isolates, 23 P. aeruginosa were recovered from patients with burn wound infections attending different hospitals in Tanta, Egypt. The susceptibility test revealed that 95.7% of P. aeruginosa isolates were MDR with a high incidence of resistance against carbenicillin. Antibacterial activities of silver nanoparticles (Ag-NPs) against the isolates examined showed various inhibition zone diameters ranging from 11 to 17 mm. Strong synergistic efficacy of neomycin was reported in combination with Ag-NPs against MDR P. aeruginosa P8 and P14 isolates. The in vivo effectiveness of various pharmaceutical formulations prepared from a combination of neomycin antibiotic with Ag-NPs in the treatment of induced bacterially infected mice burns showed that maximum healing activity along with faster wound contraction reported with the combination of neomycin-Ag-NPs in the spray formulation. Generally, data indicated that incorporating Ag-NPs in combination with certain antibiotics may be a new, promising application for wound treatments, especially burns infected with MDR P. aeruginosa.

Biosynthesis of Silver Nanoparticles by Marine Actinobacterium Nocardiopsis dassonvillei and Exploring Their Therapeutic Potentials

“…Energy Dispersive X-ray (EDX) was performed to identify the elemental composition of the AgNPs ( Goel et al, 2021 ). Transmission electron microscopy (TEM; JEM-1230, JEOL, Japan) with a 120 kV acceleration voltage was used to determine the size, distribution, and morphology of the Bio-AgNPs ( Khalil et al, 2021 ). The average size and surface charge of the Bio-AgNPs was determined using dynamic light scattering (DLS) ( Goel et al, 2021 ).…”

“…Silver, among various metals, has long been recognized in applications, including food preservation, antimicrobial therapy, and water purification ( Otari et al, 2015 ; Makvandi et al, 2019 ). Recent advancements in nanotechnology have resulted in the widespread use of silver nanoparticles (AgNPs) as antimicrobial, anti-inflammatory, and anti-cancerous agents ( Khalil et al, 2021 ), since AgNPs have unique optical, magnetic, catalytic, and electronic properties ( Sánchez-López et al, 2020 ).…”

“…For the synthesis of AgNPs, various physical and chemical methods such as microwave assistance, thermal decomposition, and chemical reduction have been employed. However, these conventional methods are energy intensive, use hazardous chemicals, are time consuming, and pose environmental and biological risks, precluding their therapeutic applications ( Otari et al, 2015 ; Bahlol et al, 2019 ; Khalil et al, 2021 ). On the other hand, biological methods are simple, quick, and inexpensive approaches to synthesize nanostructure materials as well as to reduce the generation of hazardous substances toxic to the environment and human health ( Singh et al, 2020 ; Nikzamir et al, 2021 ).…”

“…Multidrug resistance (MDR) is a persistent global issue. A considerable need for revolutionary nanomaterials has developed for the construction of new antimicrobial biomaterials, which has unavoidably opened up new therapeutic horizons in medical approaches ( Khalil M. A. et al, 2015 ; El-Zawawy and Ali, 2016 ; Khalil et al, 2020 , 2021 ). To the best of our knowledge, no studies have been published on the efficacy of Bio-AgNPs produced by the marine actinobacterium Nocardiopsis dassonvillei as an antimicrobial candidate against MDR pathogens with concurrent antioxidant, insecticidal, and anticancer potential.…”

Advances in the Application of Nanomaterials as Treatments for Bacterial Infectious Diseases

“…Ag NPs have shown synergic action with colistin, and imipenem against pandrugresistant A. baumannii isolated from patients [106]. The efficacy of combined neomycin, an antibiotic with Ag-NPs in bacteria-infected mice with burn wounds revealed good healing ability with excellent wound contraction when the neomycin-Ag-NPs were used in the spray method [107]. Ag NPs@organic frameworks/graphene oxide in sericin/chitosan/polyvinyl alcohol hydrogel were constructed for the redox ability of tyrosine residues in silk sericin without extra chemicals [108].…”