Evaluation of Antibacterial Activity of Urtica dioica L. Leaf Ethanolic Extract Using Agar Well Diffusion and Disc Diffusion Methods

Mirtaghi, Seyedeh Masoumeh; Nejad, Parisa Torbati; Mazandarani, M; Livani, F; Bagheri, Hanieh

doi:10.18869/acadpub.mlj.10.5.15

Cited by 11 publications

(6 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…So, according to studies by Namazi et al (2018), 70% ethanolic extract of Urtica dioica L. prevents the occurrence of atherosclerotic lesions in the aorta rats, which is associated with a positive effect on the lipid profile of the serum, but does not significantly affect the state of antioxidants and manifestations of oxidative stress. On the other hand, Mirtaghi et al (2016) noted that 70% ethanolic extract of nettle (Urtica dioica L.) is characterized by antibacterial and antioxidant activity, which may be due to the high content of phenolic compounds and the presence of alkaloids, tannins, and terpenoids in its composition, which confirms the use of this plant for the treatment of urinary tract infections. Johnson et al (2013) indicate that to obtain the desired anti-inflammatory effect, the nettle extraction was carried out with a mixture of water, hexane, methanol, and dichloromethane.…”

Section: Discussionmentioning

confidence: 98%

Metabolic processes in the organism of animals under the action of plant extract

Buchko

Havryliak

Yaremkevych

et al. 2019

Regul. Mech. Biosyst.

View full text Add to dashboard Cite

The possibility of increasing metabolism and resistance in an organism of animals under the influence of biologically active substances of natural origin is considered in the paper. In the current study, we investigated the effect of 40% ethanolic extract of common nettle (Urtica dioica L.) on hematologic indexes (hemoglobin concentration, erythrocytes and leukocytes content), protein metabolism (total protein, aspartate aminotransferase (AST), alanine aminotransferase activities (ALT)), glutathione metabolism (peroxidase (GP), glutathione reductase (GR) and reduced glutathione content (GSH)), antioxidant defense systems (superoxide dismutase (SOD), catalase (CAT)), and indicators of free radical processes (lipid hydroperoxidation products (LHP), TBA-active products and carbonyl groups of protein (CP)) in the blood and tissues of normal and adrenalin-stressed rats. White Wistar rats (male) with body weight 180–200 g were used in the experiments. The animals were divided into 4 groups (control and 3 experimental), each containing 7 animals. The animals of experimental groups D2 and D3 received 40% ethanolic extract of common nettle in a dose of 5 mL/kg of body weight during 4 weeks. After 29 days of the experiment, the animals of D1 and D3 groups were intramuscularly administered 0.1% solution of adrenaline hydrochloride at a dose of 1 mg/kg of body weight. One day after the administration of adrenaline, the animals were decapitated under ether anesthesia. The objects of the study were blood and homogenates of liver, heart, and kidneys of the rats. Our results have shown that adrenaline stress causes a decrease in the concentration of hemoglobin, LHP, SOD activity and increase in activities of AST, GP, GR, and GSH content in the blood of rats. Stress induced by adrenaline was accompanied by decrease in CP content, activities of SOD, CAT, and increase in the glutathione chain of antioxidant defense system in the liver of the animals while in the heart and kidneys, there was an increase in oxidative stress parameters and decrease in antioxidant enzyme activities (SOD, CAT, GP, GR). The administration of ethanolic extract of nettle caused an increase within the physiological range in the erythrocytes and leucocytes content, hemoglobin concentration, and normalization of protein metabolism (decrease in the activity of AST and increase in protein concentration) in the blood of the stressed rats. The activation of the antioxidant defense system (increase in activities of SOD, GP, CAT, GR) and inhibition of free radical processes (decrease in concentration of LHP, TBA-active products and CP) was observed in the tissues of animals of D2 and D3 groups. Our results suggested that using a complex of biologically active substances of the common nettle ethanolic extract prevents metabolic disorders and stresses of different etiology through the activation of the antioxidant defense system, the normalization of protein metabolism and stimulation of hematopoiesis both in normal and stressed rats.

show abstract

Section: Discussionmentioning

confidence: 98%

Metabolic processes in the organism of animals under the action of plant extract

Buchko

Havryliak

Yaremkevych

et al. 2019

Regul. Mech. Biosyst.

View full text Add to dashboard Cite

show abstract

“…The experiment was repeated 3 times, and the inhibition zones were recorded as mean standard error. [19,20]…”

Section: Agar Well Diffusion Methodsmentioning

confidence: 99%

Untitled

2022

IJGP

View full text Add to dashboard Cite

Introduction:Herbal medicines are being used since ancient times. Although herbal medications are considered safe, their authenticity and purity have been questioned. It is essential to establish relevant SOPs at the source to ensure the authenticity, purity, quality, and efficacy of herbal medications. Antibiotic resistance is developing every day and is now considered a severe health hazard all over the world, so new medications from natural sources are needed. Therefore, the objective of the study was to develop the SOPs and to evaluate the antimicrobial activity of Qurṣ-i Bel (QB). Materials and Methods: In the present study, 12 batches of QB were prepared to develop SOPs with Loabe Samaghe Arabi (mucilage of Acacia arabica) (GAM), a 100 mesh sieve, and various temperatures and drying times employed for each batch. The antimicrobial activity was done using the agar well diffusion method against four pathogenic bacteria, that is, Staphylococcus aureus, Escherichia coli, Klebsiella species, and Pseudomonas species. Results: The final batch (batch no.12), in which 15% w/w GAM used as a binding agent and dried at 90°C for 120 min showed hardness 17.66 ± 0.57-18.33 ± 1.15 kg/cm 2 , friability 0.0192 ± 0.00-0.0199 ± 0.00 %, and disintegration time 14.00 ± 0.00 min. The findings revealed that the methanolic and hydroalcoholic extracts of QB have antibacterial action against the three pathogens examined (Staphylococcus aureus, Escherichia coli, and Pseudomonas species). While, Klebsiella pneumoniae showed no zone of inhibition for all concentrations in all three extracts and aqueous extract was found ineffective against all tested organisms and showed no zone of inhibition. Conclusion: Its SOPs might be employed as traditional medicine for the treatment of various infections and could be used as a future reference for process standardization.

show abstract

“…This might be caused by the power of the silver ions to penetrate into the cell membrane of the E. coli. The cell membrane of Gram-negative bacteria was built by hydrophilic groups and acted as a barrier against hydrophilic dyes, antibiotics, and detergents [14]. Another study reported that the inactive antibacterial activity could be owing to the low concentrations or the presence of lipopolysaccharide in the surface membrane of Gram-negative bacteria, which had a role as a permeability barrier and restricted diffusion of active compounds [15].…”

Section: Bsmentioning

confidence: 99%

Comparative study of well diffusion and disc diffusion method to investigate the antibacterial properties of silver nanoparticles synthesized from Curcuma longa extracts

Nurkhaliza,

Risana,

Pubasari

et al. 2024

E3S Web Conf.

View full text Add to dashboard Cite

Green synthesis method for the preparation of silver nanoparticles (AgNPs) using Curcuma longa extracts as reducing and stabilizing agents was studied. The optimization of the synthesis was determined by varying the weight of the extracts, the AgNO3 concentration, and the reaction time. The results revealed that 3 g of extracts, 0.005 M AgNO3, and 30 min of incubation time were the ideal conditions to synthesize AgNPs-Cur. The characterization was assessed by UV-Visible spectroscopy, FTIR, and TEM. UV-Vis spectra showed a surface plasmon resonance (SPR) peak at 428 nm with the stability after 5 months of storage time. FTIR analysis revealed the formation of AgNPs using turmeric extracts and the synthesized AgNPs-Cur had the average size of 22.12 ± 0.60 nm (analyzed by TEM). The well diffusion and disc diffusion method were performed to investigate the antibacterial activity of AgNPs-Cur against Bacillus subtilis, Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa. The results concluded that AgNPs-Cur exhibited better bacterial growth inhibitory activities with well diffusion method than disc diffusion method.

show abstract

Evaluation of Antibacterial Activity of Urtica dioica L. Leaf Ethanolic Extract Using Agar Well Diffusion and Disc Diffusion Methods

Cited by 11 publications

References 6 publications

Metabolic processes in the organism of animals under the action of plant extract

Metabolic processes in the organism of animals under the action of plant extract

Untitled

Comparative study of well diffusion and disc diffusion method to investigate the antibacterial properties of silver nanoparticles synthesized from Curcuma longa extracts

Contact Info

Product

Resources

About