Evaluation of antiaggregatory activity of flavonoid aglycone series

Bojić, Mirza; Debeljak, Željko; Tomičić, Maja; Medić-Šarič, Marica; Tomić, Siniša

doi:10.1186/1475-2891-10-73

Cited by 47 publications

(42 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…() also demonstrated, using a large set of flavonoids at physiologically attainable concentrations, the inhibition of ADP‐induced platelet aggregation. These data suggest that regular intake of flavonoid rich compounds could improve platelet function (Bojic et al ., ). The short‐term (i.e.…”

Section: Polyphenols and Platelet Functionmentioning

confidence: 97%

A review of the mechanisms and effectiveness of dietary polyphenols in reducing oxidative stress and thrombotic risk

Santhakumar

Bulmer

Singh

2013

J Human Nutrition Diet

124

View full text Add to dashboard Cite

Dietary sources of polyphenols, which are derivatives and/or isomers of flavones, isoflavones, flavonols, catechins and phenolic acids, possess antioxidant properties and therefore might be important in preventing oxidative-stress-induced platelet activation and attenuating adverse haemostatic function. Free radicals, including reactive oxygen and nitrogen species, promote oxidative stress, leading to platelet hyperactivation and the risk of thrombosis. The consumption of antioxidant/polyphenol rich foods might therefore impart anti-thrombotic and cardiovascular protective effects via their inhibition of platelet hyperactivation or aggregation. Most commonly-used anti-platelet drugs such as aspirin block the cyclooxygenase (COX)-1 pathway of platelet activation, similar to the action of antioxidants with respect to neutralising hydrogen peroxide (H2 O2 ), with a similar effect on thromboxane production via the COX-1 pathway. Polyphenols also target various additional platelet activation pathways (e.g. by blocking platelet-ADP, collagen receptors); thus alleviating fibrinogen binding to platelet surface (GPIIb-IIIa) receptors, reducing further platelet recruitment for aggregation and inhibiting platelet degranulation. As a result of the ability of polyphenols to target additional pathways of platelet activation, they may have the potential to substitute or complement currently used anti-platelet drugs in sedentary, obese, pre-diabetic or diabetic populations who can be resistant or sensitive to pharmacological anti-platelet therapy.

show abstract

Section: Polyphenols and Platelet Functionmentioning

confidence: 97%

A review of the mechanisms and effectiveness of dietary polyphenols in reducing oxidative stress and thrombotic risk

Santhakumar

Bulmer

Singh

2013

J Human Nutrition Diet

124

View full text Add to dashboard Cite

show abstract

“…In this study, the phytochemical screening showed ( Table 1) the presence of polyphenols, especially tannins and flavonoids (flavones, flavanones, isoflavones and chalcones), which were already associated with hemostasis alteration. The antiplatelet activity can be attributed to the increased production of prostacyclin by endothelian cells, and its decreasing aggregation via synthesis of cAMP, and the increased concentration of cAMP, therefore inhibiting the expression of platelet GPIIb/IIIa receptors (Bojić et al, 2011). To show the anticoagulant effect of Plinia cauliflora (DC.)…”

Section: Resultsmentioning

confidence: 99%

Preliminary Studies on Anticoagulation and Antiplatelet Activities of Plinia cauliflora (Mart.) Kausel Leaves Extract

Tanelli¹,

Belardo²,

Peiró³

et al. 2017

J App Pharma Sci

View full text Add to dashboard Cite

The objective of this study was to evaluate the effect of aqueous extracts of Plinia cauliflora (DC.) Kausel leaves in different concentrations (0.5 to 10.0% (w/v)), on the hemostasis through changes in platelet aggregation and blood coagulation. Has been assessed by phytochemical screening (total phenols; tannins; flavonoids; alkaloids and saponins) of Plinia cauliflora (DC.) Kausel leaves using standard methodologies. The human blood by discard sample was used. The coagulation assay was carried out with 'thromboplastin hemostasis' for prothrombin time (PT) and activated partial thromboplastin time (aPTT) commercial kit, where platelets were determined by complete blood count (CBC) analyzer. The phytochemical screening showed the presence of total phenols; tannins and flavonoids. In assessing the anticoagulant activity, the aqueous extracts of P. cauliflora (DC.) Kausel leaves in different concentrations (2.0%; 2.5%; 5.0% and 10.0% (w/v) didn't show any coagulation and could not be measured on the analyzer. Hemogram (CBC) of the sample results showed a significant reduction in platelet concentration, especially in aqueous extracts 2.5%, while the other parameters did not seem to be significantly affected. Based on these preliminary results, it can be suggested that the aqueous extract of this species has anticoagulant and antiplatelet activities.

show abstract

“…The anterior can be explained by the presence of nucleosides and flavonoids in aqueous fraction. Nucleosides and flavonoids act on adenylate cyclase participation with increase of cAMP levels (antiplatelet activity) [42, 43]. Also O'Kennedy established that tomato extracts inhibit platelet P-selectin expression after being stimulated by ADP [34].…”

Section: Discussionmentioning

confidence: 99%

Protective Mechanisms ofS. lycopersicumAqueous Fraction (Nucleosides and Flavonoids) on Platelet Activation and Thrombus Formation:In Vitro,Ex VivoandIn VivoStudies

Fuentes

Pereira

Alarcón

et al. 2013

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

The purpose of this research was to investigate mechanisms of antiplatelet action of bioactive principle from S. lycopersicum. Aqueous fraction had a high content of nucleosides (adenosine, guanosine, and adenosine 5′-monophosphate) by HPLC analysis. Also aqueous fraction presented flavonoids content. Aqueous fraction inhibited platelet activation by 15 ± 6% (P < 0.05). Fully spread of human platelets on collagen in the presence of aqueous fraction was inhibited from 15 ± 1 to 9 ± 1 μm2 (P < 0.001). After incubation of whole blood with aqueous fraction, the platelet coverage was inhibited by 55 ± 12% (P < 0.001). Platelet ATP secretion and aggregation were significantly inhibited by the aqueous fraction. At the same concentrations that aqueous fraction inhibits platelet aggregation, levels of sCD40L significantly decreased and the intraplatelet cAMP levels increased. In addition, SQ22536, an adenylate cyclase inhibitor, attenuated the effect of aqueous fraction toward ADP-induced platelet aggregation and intraplatelet level of cAMP. Platelet aggregation ex vivo (human study) and thrombosis formation in vivo (murine model) were inhibited by aqueous fraction. Finally, aqueous fraction may be used as a functional ingredient adding antiplatelet activities (nucleosides and flavonoids) to processed foods.

show abstract

Evaluation of antiaggregatory activity of flavonoid aglycone series

Cited by 47 publications

References 27 publications

A review of the mechanisms and effectiveness of dietary polyphenols in reducing oxidative stress and thrombotic risk

A review of the mechanisms and effectiveness of dietary polyphenols in reducing oxidative stress and thrombotic risk

Preliminary Studies on Anticoagulation and Antiplatelet Activities of Plinia cauliflora (Mart.) Kausel Leaves Extract

Protective Mechanisms ofS. lycopersicumAqueous Fraction (Nucleosides and Flavonoids) on Platelet Activation and Thrombus Formation:In Vitro,Ex VivoandIn VivoStudies

Contact Info

Product

Resources

About