Evaluation of anti-nociceptive and anti-inflammatory activity of low-level laser therapy on temporomandibular joint inflammation in rodents

Barretto, Sandra Regina; Melo, Genecy Calado de; Santos, José Ribeiro dos; Oliveira, Makson Gleydson Brito de; Pereira-Filho, Rose Nely; Alves, Ângela Valéria Farias; Ribeiro, Maria Amália Gonzaga; Lima-Verde, I.B.; Quintans‐Júnior, Lucindo José; Albuquerque‐Júnior, Ricardo Luiz Cavalcanti de; Bonjardim, Leonardo Rigoldi

doi:10.1016/j.jphotobiol.2013.10.002

Cited by 34 publications

(36 citation statements)

References 46 publications

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“…PBM is a potential drug-free, non-invasive application of light widely used for the treatment of a variety of pathophysiological conditions, including healing of chronic wounds, muscles and nerve injuries, reduction of inflammation, pain and restoration of function [13]. In PBM therapy, successful therapeutic outcomes require selection of optimum optical treatment protocols including illumination parameters (such as wavelength, fluence, fluence rate, pulse structure etc.)…”

Section: Introductionmentioning

confidence: 99%

Photobiomodulation with Pulsed and Continuous Wave Near-Infrared Laser (810 nm, Al-Ga-As) Augments Dermal Wound Healing in Immunosuppressed Rats

et al. 2016

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Chronic non-healing cutaneous wounds are often vulnerable in one or more repair phases that prevent normal healing and pose challenges to the use of conventional wound care modalities. In immunosuppressed subject, the sequential stages of healing get hampered, which may be the consequences of dysregulated or stagnant wound inflammation. Photobiomodulation (PBM) or low-level laser (light) therapy (LLLT) emerges as a promising drug-free, non-invasive biophysical approach for promoting wound healing, reduction of inflammation, pain and restoration of functions. The present study was therefore undertaken to evaluate the photobiomodulatory effects of 810 nm diode laser (40 mW/cm2; 22.6 J/cm2) with pulsed (10 and 100 Hz, 50% duty cycle) and continuous wave on full-thickness excision-type dermal wound healing in hydrocortisone-induced immunosuppressed rats. Results clearly delineated that 810 nm PBM at 10 Hz was more effective over continuous and 100 Hz frequency in accelerating wound healing by attenuating the pro-inflammatory markers (NF-kB, TNF-α), augmenting wound contraction (α-SM actin), enhancing cellular proliferation, ECM deposition, neovascularization (HIF-1α, VEGF), re-epithelialization along with up-regulated protein expression of FGFR-1, Fibronectin, HSP-90 and TGF-β2 as compared to the non-irradiated controls. Additionally, 810 nm laser irradiation significantly increased CCO activity and cellular ATP contents. Overall, the findings from this study might broaden the current biological mechanism that could be responsible for photobiomodulatory effect mediated through pulsed NIR 810 nm laser (10 Hz) for promoting dermal wound healing in immunosuppressed subjects.

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Section: Introductionmentioning

confidence: 99%

Photobiomodulation with Pulsed and Continuous Wave Near-Infrared Laser (810 nm, Al-Ga-As) Augments Dermal Wound Healing in Immunosuppressed Rats

et al. 2016

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“…joint inflammation situations. [8][9][10] It can modulate proinflammatory mediators, thereby decreasing the expression of cytokines such as IL-1b, IL-6, and TNF-a, and it also can decrease the number of inflammatory cells (macrophages and neutrophils). 8,11 LLLT was introduced as an alternative noninvasive treatment for RA approximately two decades ago.…”

Section: Introductionmentioning

confidence: 99%

The Fluence Effects of Low-Level Laser Therapy on Inflammation, Fibroblast-Like Synoviocytes, and Synovial Apoptosis in Rats with Adjuvant-Induced Arthritis

Hsieh

Cheng

Huang

et al. 2014

Photomedicine and Laser Surgery

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Objective: The aim of this study was to evaluate the effect of low-level laser therapy (LLLT) operating at low and high fluences on joint inflammation, fibroblast-like synoviocytes (FLS), and synovial apoptosis in rats with adjuvant-induced arthritis. Background data: Rheumatoid arthritis (RA) is characterized by pronounced inflammation and FLS proliferation within affected joints. Certain data indicate that LLLT is effective in patients with inflammation caused by RA; however, the fluence effects of LLLT on synovium are unclear. Methods: Monoarthritis was induced in adult male Sprague-Dawley rats (250-300 g) via intraarticular injection of complete Freund's adjuvant (CFA) into the tibiotarsal joint. Animals were irradiated 72 h after CFA administration with a 780 nm GaAlAs laser at 4.5 J/cm 2 (30 mW, 30 sec/spot) and 72 J/cm 2 (80 mW, 180 sec/spot) daily for 10 days. After LLLT, the animals were euthanized and their arthritic ankles were collected for histopathological analysis, immunoassays of tumor necrosis factor (TNF)-a, matrix metallopeptidase (MMP)3 and 5B5, and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assays. Results: LLLT at a fluence of 4.5 J/cm 2 significantly reduced infiltration of inflammatory cells and expressions of TNF-a-, MMP3-and 5B5-like immunoreactivities, as well as resulting in more TUNEL-positive apoptotic cells in the synovium. No significant changes were observed in these biochemicals and inflammation in arthritic animals treated with 72 J/cm 2 . Conclusions: LLLT with low fluence is highly effective in reducing inflammation to sites of injury by decreasing the numbers of FLS, inflammatory cells, and mediators in the CFA-induced arthritic model. These data will be of value in designing clinical trials of LLLT for RA.

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“…It is shown the anti-nociceptive and anti-inflammatory effect of infrared light at a visceral pain, pain in the temporomandibular joint, formalin-induced inflammatory pain in extremity, pain in mice with closed craniocerebral trauma et al [16][17][18]. However, the analgesic effect observed in our experiments using the blue and infrared LEDs (50.1%), cannot be attributed solely to the IR irradiation.…”

Section: Effects Of CL Blue and Blue+irmentioning

confidence: 59%

Analgesic and Sedative Effects of Blue LED Light in Combination with Infrared LED Irradiation

Gulyar¹,

Tamarova²

2017

UCMS

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INTRODUCTION: Our previous studies have shown that polarized red+infrared LED light causes analgesic effect. The aim of this study was to test whether the blue LED light has such effect. We compared the analgesic efficacy of continuous blue light without infrared component and in combination with infrared irradiation. We also determined the dependence of responses on frequency of pulsation of blue+infrared (blue+IR) LED irradiation. METHODS: We used the model of experimentally induced chemical inflammation (formalin test). The source of the light emission was MEDOLIGHT-BluDoc device. In animals (white mice males), we recorded the duration of pain (licking the affected limb) and non-painful (sleeping, washing, running, food) behavioral reactions with placebo (control) and after the 10-minute light application on the locus of pain. The influence of continuous (blue or blue+IR) and pulsed (frequency of pulsations 10 Hz, 600 Hz, 3000 Hz and 8000 Hz) (blue+IR) light was determined. RESULTS: After the application of blue LED light without infrared component or with infrared radiation duration of pain response decreased to 317.3 s and 282.3 s against 566.2 s in the control group. Analgesia was 43.6% and 50.1%. Pulsed blue+IR LED light (all the tested ranges) also significantly reduced pain. Analgesia was maximal at a frequency of 8000 Hz (57.6%) and minimal at a frequency of 10 Hz (38.3%). Duration of sleep in experimental groups was 2.3-3.8 times longer than in the control group, the effect was most noticeable at frequency of 10 Hz. Conclusion: It was found that the blue and blue+IR LED light MEDOLIGHT-BluDoc device (both continuous and pulsing mode) when applied to a painful inflammation area possess a significant analgesic and sedative effect.

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Evaluation of anti-nociceptive and anti-inflammatory activity of low-level laser therapy on temporomandibular joint inflammation in rodents

Cited by 34 publications

References 46 publications

Photobiomodulation with Pulsed and Continuous Wave Near-Infrared Laser (810 nm, Al-Ga-As) Augments Dermal Wound Healing in Immunosuppressed Rats

Photobiomodulation with Pulsed and Continuous Wave Near-Infrared Laser (810 nm, Al-Ga-As) Augments Dermal Wound Healing in Immunosuppressed Rats

The Fluence Effects of Low-Level Laser Therapy on Inflammation, Fibroblast-Like Synoviocytes, and Synovial Apoptosis in Rats with Adjuvant-Induced Arthritis

Analgesic and Sedative Effects of Blue LED Light in Combination with Infrared LED Irradiation

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