Evaluation of Anti‐Inflammatory Properties of Isoorientin Isolated from Tubers of Pueraria tuberosa

Abstract

Inflammation is the major causative factor of different diseases such as cardiovascular disease, diabetes, obesity, osteoporosis, rheumatoid arthritis, inflammatory bowel disease, and cancer. Anti-inflammatory drugs are often the first step of treatment in many of these diseases. The present study is aimed at evaluating the anti-inflammatory properties of isoorientin, a selective cyclooxygenase-2 (COX-2) inhibitor isolated from the tubers of Pueraria tuberosa, in vitro on mouse macrophage cell line (RAW 264.7) and in vivo on mouse paw edema and air pouch models of inflammation. Isoorientin reduced inflammation in RAW 264.7 cell line in vitro and carrageenan induced inflammatory animal model systems in vivo. Cellular infiltration into pouch tissue was reduced in isoorientin treated mice compared to carrageenan treated mice. Isoorientin treated RAW 264.7 cells and animals showed reduced expression of inflammatory proteins like COX-2, tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), 5-lipoxygenase (5-LOX), and interleukin 1-β (IL-1-β) both in vitro and in vivo. The antioxidant enzyme levels of catalase and GST were markedly increased in isoorientin treated mice compared to carrageenan treated mice. These results suggest that isoorientin, a selective inhibitor of COX-2, not only exerts anti-inflammatory effects in LPS induced RAW cells and carrageenan induced inflammatory model systems but also exhibits potent antioxidant properties.

1H‐NMR metabolomics for evaluating the protective effect of Clinacanthus nutans (Burm. f) Lindau water extract against nitric oxide production in LPS‐IFN‐γ activated RAW 264.7 macrophages

“…According to Xiao et al, orientin suppressed the TNF‐ α , IL‐6, IL‐18, and IL‐1 β production mediated by LPS in RAW 264.7 cells as well as the expression of COX‐2 and i NOS through the inhibition of NF‐ k B pathway. Isoorientin successfully reduced the expression of TNF‐ α , IL‐6, 5‐lipoxygenase (5‐LOX), and IL‐1 β in both LPS stimulated RAW cell and carrageenan induced inflammatory model . For vitexin, isovitexin and schaftoside, though sonicated absolute ethanol and water extracts possessed significant higher concentration, other method prepared extracts showed considerable amount of vitexin, isovitexin and schaftoside too which further supported their location in the positive side of PC2 in the PLS biplot.…”

“…Isoorientin successfully reduced the expression of TNF-α, IL-6, 5-lipoxygenase (5-LOX), and IL-1β in both LPS stimulated RAW cell and carrageenan induced inflammatory model. 46 For vitexin, isovitexin and schaftoside, though sonicated absolute ethanol and water extracts possessed significant higher concentration, other method prepared extracts showed considerable amount of vitexin, isovitexin and schaftoside too which further supported their location in the positive side of PC2 in the PLS biplot. Isovitexin was the most abundant in sonicated absolute ethanol extract.…”

Impact of Isoorientin on Metabolic Activity and Lipid Accumulation in Differentiated Adipocytes

“…Such limitations have raised interest in expanding research into the use of natural products as therapeutic agents to prevent obesity or its associated complications. Although some studies conducted over the years showed isoorientin, or plants rich in this natural compound, can ameliorate obesity-related complications, such as oxidative stress, inflammation, hyperlipidemia, and insulin resistance [5,[16][17][18][19][20], there is still relatively little information about its impact as a pure compound on obesity.…”

Determination of isoorientin levels in rat plasma after oral administration of Vaccinum bracteatum Thunb. methanol extract by high‐performance liquid chromatography‐tandem mass spectrometry

“…In a follow-up study, we isolated isoorientin, a representative flavonoid glycoside, from VBME (Kwon et al, 2016). The anti-inflammatory properties of isoorientin have been attributed to its selective cyclooxygenase-2 inhibitory effects (Anilkumar et al, 2017;Sumalatha et al, 2015).…”