Evaluation of anthelmintic antimicrobial and antioxidant activity of Chenopodium album

Abstract

The objective of this study was to evaluate the anthelmintic, antimicrobial and antioxidant activities of Chenopodium album against gastrointestinal nematodes of sheep and some pathogenic microbes. A worm motility inhibition assay was used for in vitro study, and a faecal egg count reduction assay was used for an in vivo study. Various concentrations ranging from 100 to 500 μg/ml of the extract were subjected to antimicrobial screening by disc diffusion method against four selected bacterial (Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas multocida and Escherichia coli) and two fungal (Aspergillus flavus and Candida albicans) strains in order to estimate the medicinal potential of the herb. DPPH (1,1-diphenyl-2-picrylhydrazyl), riboflavin photo-oxidation, deoxyribose, lipid peroxidation assays were used for antioxidant activity. The extracts exhibited dose- and time-dependent anthelmintic effects on the Haemonchus contortus as compared to levamisole. The extract showed maximum inhibitory effect against S. aureus (28 ± 0.14 mm), while as mild inhibitory effect was observed against E. coli among the selected microbial strains. The effect produced by the different extract concentrations was comparable with the standard antibacterial agent streptomycin sulphate and antifungal agent nystatin, which were used as effective positive control in the study. The antioxidant activity showed that the extracts exhibited scavenging effect in concentration-dependent manner on superoxide anion radicals and hydroxyl radicals leading to the conclusion that the plant has broad spectrum anthelmintic, antimicrobial and antioxidant activities and could be a potential alternative for treating various diseases.

Edible halophytes of the Mediterranean basin: Potential candidates for novel food products

“…With respect to medicinal properties, Salam Jekendra, Joylani, Rebika, & Priyadarshini (2011) observed that C. album extracts exhibited higher antioxidant activity (IC 50 = 206 μg/ml) than vitamin C (IC 50 = 15.1 μg/ml) and quercetin (IC 50 = 6.45 μg/ml). Besides, Lone et al (2017) observed that the extracts of C. album exhibited considerable antimicrobial activity against Staphylococcus aureus, Pseudomonas multocida and Escherichia coli. Moreover, Beyrouthy, Nelly, Annick, and Frederic (2008) have described the use of alcoholic decoctions of aerial parts against rheumatism and arthritis in traditional medicine of Lebanon, while Ksouri et al (2012) have reported unspecified medicinal uses of the species, mostly in the region of Albania.…”

An Investigation of Antibacterial and Antioxidant Activity of Nettle (Urtica dioica L.), Mint (Mentha piperita), Thyme (Thyme serpyllum) and Chenopodium album L. Plants from Yaylacık Plateau, Giresun, Turkey

“…In the study of Lone et al (2017), DPPH radical scavenging activity of the methanol extracts of C. album ranged from 45% to 73% at concentrations varying from 50 to 300 µg/mL, whereas DPPH radical scavenging activity of the aqueous extracts of C. album ranged from 84% to 96% at concentrations between 50-300 µg/mL (Lone et al, 2017). In our study, DPPH radical scavenging activity of the extracts of C. album ranged from 9.28% to 24.90%.…”

“…The researchers also stated that essential oils of Thymus strains are highly active disinfectants so they can be used as evaporators, which can be applied against different human pathogenic bacteria and yeasts. Lone et al (2017) showed that methanol extract showed maximum antibacterial action towards S. aureus, while as mild inhibitory effect was revealed against E. coli among the tested strains.…”

In vitro anthelmintic activity of Chenopodium album and in-silico prediction of mechanistic role on Eisenia foetida

“…The medicinal plants contain various secondary metabolites that exhibit anthelmintic activity [36,37,38]. The plant Chenopodium album plant was earlier reported for its anthelmintic activity [14,15,16]. Nevertheless, the responsible secondary metabolites and their mechanism for anthelmintic activity have never been explored.…”

“…[11,12,13]. The plant was reported for its anthelmintic activity [14,15,16]. Nevertheless, the responsible secondary metabolites and their mechanism for anthelmintic activity have never been explored.…”