Evaluation of an interactive computer program to teach gram-stain interpretation

Mandel, Lynn S.; Schaad, Douglas C.; Cookson, Brad T.; Curtis, Janet D.; Orkand, Adam R.; Wener, Mark H.; LeCrone, Carol N.; Dewitt, Dawn; Astion, Michael L.

doi:10.1097/00001888-199610000-00058

Cited by 7 publications

(3 citation statements)

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“…This can be the baseline for treatment initiation and initial choice of antibiotic therapy. 8,9 In Ethiopia, most medical laboratories in healthcare facilities cannot create microbial cultures to diagnose microbial infections. This is because of a lack of skilled manpower, infrastructure, cost of culture diagnostic materials, and other issues.…”

Section: Introductionmentioning

confidence: 99%

Competency Assessment on Gram Stain Examination and Interpretation Among Medical Laboratory Professionals Working in Selected Hospitals of Addis Ababa, Ethiopia

Tsehay,

Sineshaw,

Eshetu

et al. 2024

JMDH

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Background Laboratory test results are the cornerstone for patient diagnosis and treatment. Gram staining is a classic laboratory test method used to differentiate between bacteria. Competence assessment can help identify gaps and provide suggestions to academics, researchers, and policymakers to address competency gaps. In Ethiopia, there is no evidence of competency assessment by medical laboratory professionals using the Gram-staining technique. Objective To assess the competency of medical laboratory professionals on Gram stain examination and interpretation in selected hospitals of Addis Ababa, Ethiopia. Methods A cross-sectional study was conducted to assess the competency of medical laboratory professionals on Gram stain examination and interpretation from September 2015 to December 2017. Results Of 190 participants, 55 (28.9%) participants scored low knowledge, 131 (68.9%) scored medium knowledge, and only 4 (2.1%) respondents scored high knowledge. From the study variables, education level, supervision by regional or federal government bodies, and training about Gram staining were significantly associated with the knowledge level of study participants. Forty eight (25.3%), 78 (41%), and 64 (33.7%) participants scored low, medium, and high skill level, respectively, from a total of 190 participants. From skill level analysis, hospital type, microscope type, and availability of health information resources were significantly associated with skill levels. There were 44 observations (4%) with major errors and 321 observations (28%) with very major errors from all 1140 observations. Of all observations, 321 (28.2%) reported without grading, 39 observations (3.4%) reported gram-positive bacteria as gram-negative bacteria, and 15 observations (1.4%) reported gram-negative bacteria as gram-positive bacteria. Conclusion The current study found that most medical laboratory professionals work without supervision or refresher training in Gram stain examination and interpretation. Hence, medical laboratory professionals’ knowledge and skill levels are unsatisfactory. Regular competence assessments, training, and follow-up are necessary to improve the professional competence in medical laboratories.

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Section: Introductionmentioning

confidence: 99%

Competency Assessment on Gram Stain Examination and Interpretation Among Medical Laboratory Professionals Working in Selected Hospitals of Addis Ababa, Ethiopia

Tsehay,

Sineshaw,

Eshetu

et al. 2024

JMDH

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“…Gram stain interpretation gives immediate information regarding the presence or absence of bacterial infection and morphotypes of bacteria involved in the infection. This can be baseline for initiation of treatment and initial choice of antibiotic therapy (8,9).…”

Section: Introductionmentioning

confidence: 99%

Competency Assessment on Gram Stain Examination and Interpretation among Medical Laboratory Professionals Working in Selected Hospitals of Addis Ababa, Ethiopia

Tsehay¹

2023

Preprint

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Background Laboratory test results are the cornerstone for patient diagnosis and treatment. Gram staining is one of the classic laboratory test methods actively in use to differentiate bacteria. Competence assessment could help to identify gaps and make suggestions to academicians, researchers, and policy makers to address competency gaps. In Ethiopia, no evidence about competency assessment of medical laboratory professionals on gram staining technique was available. Results From 190 participants, 55(28.9%) participants scored low knowledge, 131(68.9%) scored medium knowledge and only 4(2.1%) respondents scored high knowledge. Forty eight (25.3%), 78(41%) and 64(33.7%) participants scored low, medium and high skill level from a total of 190 participants, respectively. There were 44 observations (4%) with major errors and 321 observations (28%) with very major errors from all 1140 observations. Of all observations 321(28.2%) reported without grading, 39 observations (3.4%) reported gram positive bacteria as gram negative bacteria and 15 observations (1.4%) reported gram negative bacteria as gram positive bacteria. Conclusion Most medical laboratory professionals are working without supervision and refresher training on gram stain examination and interpretation. Hence, the knowledge level and skill level of medical laboratory professionals are not satisfactory. Regular competence assessment, training and follow up are necessary to improve medical laboratory professional competence.

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“…Information derived from a Gram stain and several simple biochemical tests can be enormous, often providing a presumptive diagnosis and significantly influencing patient care. Unfortunately the training for correctly interpreting the Gram stain is disappearing 6. The goal of this article is to review the utility of the clinical bacteriology laboratory and discuss its role in the diagnosis of common clinical pathogens.…”

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confidence: 99%

Use of the diagnostic bacteriology laboratory: a practical review for the clinician

Steinbach

Shetty

2001

Postgraduate Medical Journal

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Evaluation of an interactive computer program to teach gram-stain interpretation

Cited by 7 publications

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Competency Assessment on Gram Stain Examination and Interpretation Among Medical Laboratory Professionals Working in Selected Hospitals of Addis Ababa, Ethiopia

Competency Assessment on Gram Stain Examination and Interpretation Among Medical Laboratory Professionals Working in Selected Hospitals of Addis Ababa, Ethiopia

Competency Assessment on Gram Stain Examination and Interpretation among Medical Laboratory Professionals Working in Selected Hospitals of Addis Ababa, Ethiopia

Use of the diagnostic bacteriology laboratory: a practical review for the clinician

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