Evaluation of an IgG enzyme-linked immunosorbent assay for dengue diagnosis

Miagostovich, Marize Pereira; Nogueira, Rita Maria Ribeiro; Santos, Flávia Barreto dos; Schatzmayr, Hermann G.; Araújo, Eliane Saraiva Machado de; Vorndam, Vance

doi:10.1016/s1386-6532(99)00059-1

Cited by 120 publications

(120 citation statements)

References 14 publications

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“…All samples were collected through routine surveillance conducted in Puerto Rico from 1998 to the present. The following groups of samples were selected for the creation of three separate serum panels to be tested by the Platelia DENV NS1Ag test: (i) 63 anti-DENV-IgM-positive convalescent-phase samples (defined as samples collected Ն5 DPO) for which there was a paired acute-phase sample (collected Ͻ5 DPO) that tested positive by RT-PCR for RNA from DENV (4 positive for DENV serotype 1 [DENV1], 38 for DENV2, 20 for DENV3, and 1 for DENV4) (serum panel A); (ii) 67 acute-phase samples that tested negative for DENV by RT-PCR for which there was a paired anti-DENV-IgM-positive sample (indicating seroconversion) (serum panel B); and (iii) 40 acute-phase samples that tested positive by RT-PCR for RNA from DENV (2 for DENV1, 20 for DENV2, 16 for DENV3, and 2 for DENV4) for which there was a paired convalescent-phase sample that tested negative for anti-DENV IgM but showed a 4-fold or greater increase in the anti-DENV IgG titer compared to that in the acute-phase sample (indicating a secondary infection) (serum panel C) (16,21). Samples were characterized by previously described laboratory analyses, including MAC-ELISA and anti-DENV IgG ELISA (3,16), viral isolation from C6/36 cells (8), and multiplex real-time RT-PCR (4).…”

Section: Methodsmentioning

confidence: 99%

Utility of a Commercial Nonstructural Protein 1 Antigen Capture Kit as a Dengue Virus Diagnostic Tool

Bessoff

Phoutrides

Delorey

et al. 2010

Clin Vaccine Immunol

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Annually, over 2.5 billion people are at risk for infection with dengue virus (DENV), while between 50 and 100 million people contract the infection. There is an urgent need for alternative diagnostic tools that can detect DENV during acute infection. Recent studies have shown that DENV nonstructural protein 1 (NS1) is detectable in the blood as early as the onset of symptoms and persists well into the convalescent phase of the infection. We evaluated the utility of the Bio-Rad Platelia DENV NS1 antigen capture kit in combination with real-time reverse transcriptase PCR (RT-PCR) and an IgM antibody capture enzyme-linked immunosorbent assay (MAC-ELISA) for refining a new algorithm for the diagnosis of acute-or convalescent-phase DENV infection with a single clinical sample. We tested the Bio-Rad kit with three panels of sera. These panels were designed to evaluate the sensitivities of the NS1 kit for (i) early-convalescent-phase samples, (ii) acute-phase samples with false-negative PCR results, and (iii) IgM-negative convalescent-phase samples from patients with confirmed secondary DENV infections. Results show that NS1 can be detected in 22% of serum samples collected more than 10 days after the onset of illness and in 22% of samples that did not elicit an IgM response. Additionally, NS1 was detected in 37% of the tested acute-phase samples with false-negative PCR results, suggesting that NS1 detection may be valuable in increasing the sensitivity of current acute-phase diagnostics. These results will improve diagnosis with a single acute-phase or early-convalescent-phase sample for disease surveillance and clinical diagnosis.

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Section: Methodsmentioning

confidence: 99%

Utility of a Commercial Nonstructural Protein 1 Antigen Capture Kit as a Dengue Virus Diagnostic Tool

Bessoff

Phoutrides

Delorey

et al. 2010

Clin Vaccine Immunol

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“…13 IgG antibody determinations were made using an IgG-ELISA. 14 Because the measurement of IgM antibody may fail to diagnose about 5% of secondary dengue infections, 15 specimens with borderline results by MAC-ELISA were tested by IgG-ELISA in an attempt to confirm the diagnosis by detecting an anamnestic anti-dengue antibody response.…”

Section: Methodsmentioning

confidence: 99%

The dengue and dengue hemorrhagic fever epidemic in Puerto Rico, 1994-1995.

Rigau‐Pérez

Vorndam²,

Clark³

2001

The American Journal of Tropical Medicine and Hygiene

134

120

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“…In paired samples, a full titration of 4-fold dilutions of serum was used. The endpoint titration of IgG was determined to assess seroconversion ( 5 ). Each plate was compared with a negative control serum specimen.…”

Section: The Studymentioning

confidence: 99%