Evaluation of an equine-optimized enzyme-linked immunosorbent assay for serum insulin measurement and stability study of equine serum insulin

Öberg, Josefine; Bröjer, Johan; Wattle, O.S.; Lilliehöök, Inger

doi:10.1007/s00580-011-1284-6

Cited by 27 publications

(32 citation statements)

References 24 publications

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“…Insulin and cortisol concentrations were determined using an ELISA method . This specific insulin test has been previously validated for horses . ELISA assay for cortisol determination was validated following the methodology described elsewhere .…”

Section: Methodsmentioning

confidence: 99%

Metabolic and Endocrine Profiles in Sick Neonatal Foals Are Related to Survival

Armengou

Jose‐Cunilleras

Ríos

et al. 2013

Veterinary Internal Medicne

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Background: Sick neonatal foals suffer from a variety of endocrine and metabolic derangements that may be related to outcome. There are several hepatic and lipid metabolism blood markers that have never been assessed in neonatal foals.Objectives: Assess panel of endocrine and metabolic variables in group of sick and healthy neonatal foals in order to describe their relationship with diagnosis and survival.Animals: All neonatal foals referred to Unitat Equina-Fundaci o Hospital Cl ınic Veterinari during 3 consecutive foaling seasons and a group of healthy foals.Methods: Observational prospective study. Blood samples were obtained on admission and, when possible, after 24-48 h of hospitalization and immediately before discharge or death. Measured variables were triglycerides, nonsterified fatty acids, glucose, creatinine, urea, c-glutamyltransferase, glutamate dehydrogenase (GLDH), insulin, cortisol, bile acids, and adrenocorticotropic hormone (ACTH). ACTH/cortisol and glucose/insulin ratios were calculated.Results: Urea, creatinine, and cortisol had median concentrations in septic and nonseptic foals 2-to 8-fold higher than in the control group (P < .001). Median ACTH concentration in the septic group was approximately 4 times higher than in nonseptic and control foals (P < .001). ACTH/cortisol ratio was significantly lower in sick foals compared to control foals (P < .001). A score was designed including creatinine, GLDH, and cortisol. When ! 2 of these variables were altered (P < .001), the foal had 32 times more risk of dying (OR, 31.7; 95% CI,.Conclusions and Clinical Importance: Plasma creatinine, GLDH, and cortisol should be determined in sick newborn foals on admission because of their association with survival.

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Section: Methodsmentioning

confidence: 99%

Metabolic and Endocrine Profiles in Sick Neonatal Foals Are Related to Survival

Armengou

Jose‐Cunilleras

Ríos

et al. 2013

Veterinary Internal Medicne

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“…All analyses were performed in duplicate. Intra-assay coefficients of variation for glucose, equine insulin and human insulin have been reported previously [18,22].…”

Section: Euglycaemic Hyperinsulinaemic Clampmentioning

confidence: 99%

The effect of insulin infusion on heart rate and systemic blood pressure in horses with equine metabolic syndrome

Nostell

Lindåse

Edberg

et al. 2019

Equine Veterinary Journal

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Summary Background There is little evidence that horses with equine metabolic syndrome (EMS) have higher resting blood pressures than horses with normal insulin sensitivity. However, there are indications that EMS horses have an altered dynamic response to the cardiovascular effects of insulin. Objectives To examine heart rate and the systemic blood pressure response in EMS and control horses during insulin infusion. Study design Cross‐sectional study. Methods Horses were examined with an oral sugar test (OST) and a euglycaemic hyperinsulinaemic clamp (EHC). Based on the results from the OST, the horses were classified as either EMS or control. Blood pressure was measured before the start of the EHC and at 60, 120 and 180 min of the EHC. Results There were 16 EMS and 12 control horses. Mean resting heart rate was higher for EMS horses compared with control (35.6 ± 5.1 vs. 30.3 ± 3.5 beats/min). Systolic, diastolic and mean arterial blood pressure did not differ between groups at the start of the EHC. Blood pressure decreased gradually during the EHC in the control group, whereas the EMS group showed no decline in systolic, diastolic or mean arterial pressure. Main limitations Limited number of horses. Conclusions Horses with EMS have cardiovascular changes that affect resting heart rate and systemic blood pressure during insulin infusion.

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“…The most likely explanation for this discrepancy in results is that the 2 studies used different assays for measurement of insulin (Mercordia Insulin ELISA versus Coat‐A‐Count Insulin RIA). Öberg et al showed that results between these assays were in good agreement for samples with equine insulin concentrations <160 μU/mL. On the other hand, analyses of samples from horses with higher insulin concentrations have shown to have poor agreement between different assays .…”

Section: Discussionmentioning

confidence: 99%

“…Plasma glucose concentrations were measured as a single batch using an automated analyzer . Plasma insulin concentrations were determined in duplicates using an ELISA that has previously been validated for equine insulin . The intra‐assay CV varied between 5.5% and 8.9% for each batch of insulin analyses as determined from the duplicate analyses.…”

Section: Methodsmentioning

confidence: 99%

Repeatability of the Combined Glucose‐Insulin Tolerance Test and the Effect of a Stressor before Testing in Horses of 2 Breeds

Bröjer

Lindåse

Hedenskog

et al. 2013

Veterinary Internal Medicne

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Background: The increasing number of horses diagnosed with insulin resistance (IR) and the suggested link between IR and laminitis has highlighted the need to accurately diagnose IR in clinical practice.Hypothesis/Objectives: The aim of the study was to evaluate the repeatability of the combined glucose-insulin tolerance test (CGIT) as well as to determine the effect of 2 different breeds and the effect of a stressor on the test results.Animals: Clinically normal horses, 9 Standardbred horses and 9 Icelandic horses. Methods: Prospective clinical nonrandomized trial. The CGIT was performed on all horses on 2 occasions 3 weeks apart. An additional CGIT was performed on four of the Standardbred and four of the Icelandic horses after transportation to a new environment (stressor) the day before testing.Results: Calculated parameters for the glucose curve of the CGIT had low repeatability, whereas the parameters for the insulin curve had high repeatability. There was an effect of breed (Standarbred versus Icelandic horse) as well as stress on the glucose dynamics, but not on the insulin dynamics of the CGIT.Conclusion and Clinical Importance: Repeatability of the glucose dynamics of the CGIT is low. In addition, there appears to be breed differences in the glucose dynamics. It is therefore suggested that diagnosis of IR should not be made solely based on results from the glucose curve of the CGIT.

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Evaluation of an equine-optimized enzyme-linked immunosorbent assay for serum insulin measurement and stability study of equine serum insulin

Cited by 27 publications

References 24 publications

Metabolic and Endocrine Profiles in Sick Neonatal Foals Are Related to Survival

Metabolic and Endocrine Profiles in Sick Neonatal Foals Are Related to Survival

The effect of insulin infusion on heart rate and systemic blood pressure in horses with equine metabolic syndrome

Repeatability of the Combined Glucose‐Insulin Tolerance Test and the Effect of a Stressor before Testing in Horses of 2 Breeds

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