Evaluation of an enzyme-linked immunoassay for the detection in serum of antibodies to Entamoeba histolytica

Rosenblatt, Jon E.; Sloan, Lynne M.; Bestrom, Jean E.

doi:10.1016/0732-8893(95)00045-c

Cited by 10 publications

(7 citation statements)

References 5 publications

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“…This explains the frequent occurrence of abscesses in the right hepatic lobe, which receives most of the blood draining the cecum and ascending colon (154). Some individuals presenting with ALA have concurrent amebic colitis, but more often they have no bowel symptoms, and stool microscopy is usually negative for E. histolytica trophozoites and cysts (5,152,190). Individuals can present with ALA months to years after travel or residency in an area of endemicity, so a careful travel history is mandatory (14,102,166).…”

Section: Extraintestinal Amebiasismentioning

confidence: 99%

“…This method is widely thought to be sufficient for clinical purposes, particularly for diagnosis of patients with ALA, and can be easily performed in a clinical laboratory. It may also be useful in the evaluation of intestinal and extraintestinal infections where amebiasis is suspected but organisms cannot be detected in feces (152). A microtiter ELISA to detect antibodies to E. histolytica (LMD Laboratories, Inc., Carlsbad, CA) has been shown to be 97.9% sensitive and 94.8% specific for detection of E. histolytica antibodies in ALA patients (84).…”

Section: Antibody Detection Testsmentioning

confidence: 99%

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Laboratory Diagnostic Techniques for Entamoeba Species

et al. 2007

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SUMMARY The genus Entamoeba contains many species, six of which (Entamoeba histolytica, Entamoeba dispar, Entamoeba moshkovskii, Entamoeba polecki, Entamoeba coli, and Entamoeba hartmanni) reside in the human intestinal lumen. Entamoeba histolytica is the causative agent of amebiasis and is considered a leading parasitic cause of death worldwide in humans. Although recent studies highlight the recovery of E. dispar and E. moshkovskii from patients with gastrointestinal symptoms, there is still no convincing evidence of a causal link between the presence of these two species and the symptoms of the host. New approaches to the identification of E. histolytica are based on detection of E. histolytica-specific antigen and DNA in stool and other clinical samples. Several molecular diagnostic tests, including conventional and real-time PCR, have been developed for the detection and differentiation of E. histolytica, E. dispar, and E. moshkovskii in clinical samples. The purpose of this review is to discuss different methods that exist for the identification of E. histolytica, E. dispar, and E. moshkovskii which are available to the clinical diagnostic laboratory. To address the need for a specific diagnostic test for amebiasis, a substantial amount of work has been carried out over the last decade in different parts of the world. The molecular diagnostic tests are increasingly being used for both clinical and research purposes. In order to minimize undue treatment of individuals infected with other species of Entamoeba such as E. dispar and E. moshkovskii, efforts have been made for specific diagnosis of E. histolytica infection and not to treat based simply on the microscopic examination of Entamoeba species in the stool. The incorporation of many new technologies into the diagnostic laboratory will lead to a better understanding of the public health problem and measures to control the disease.

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Section: Extraintestinal Amebiasismentioning

confidence: 99%

Section: Antibody Detection Testsmentioning

confidence: 99%

Laboratory Diagnostic Techniques for Entamoeba Species

et al. 2007

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“…During the previous decades ELISA test was compared with some of other methods such as: Indirect hemagglutination assay (IHA), indirect fluorescence assay (IFA), gel diffusion (GD), TechLab E. histolytica antigen detection test, polymerase chain reaction (PCR) and isoenzyme analysis (Rosenblatt et al 1995;Haque et al 1998;Gonin and Trudel 2003;Tanyuksel and Petri 2003). However, the use of many of the mentioned methods to the clinical laboratories is very limited or impossible in the developing countries.…”

Section: Discussionmentioning

confidence: 97%

Detection of serum antibody to Entameba histolytica in various population samples of amebic infection using an enzyme-linked immunosorbent assay

Haghighi

Rezaeian

2005

Parasitol Res

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Microscopic identification of Entameba histolytica in stool is insensitive to differentiate this pathogenic ameba from morphologically identical Entameba dispar. An enzyme-linked immunosorbent assay method was applied for diagnosing amebiasis by detection of E. histolytica antibody. A total of 258 sample sera were analyzed from five different groups including amebiasis patients, E. histolytica/E. dispar asymptomatic cyst carriers, patients clinically presumed to have intestinal and extra-intestinal amebiasis and healthy control. From 51 E. histolytica/E. dispar cysts passers identified by microscopy and culture, a positive anti-amebic antibody was measured only for eight specimens (15.6%) and 43 cyst carriers showed an absorbance less than cut-off likely due to E. dispar infection. These findings suggest usefulness of ELISA method in differentiation of Entameba species, in clinical laboratories less equipped with biological and biochemical methods or antigen capture kits.

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“…Plate ELISA is successfully applied to the serodiagnosis of amoebiasis. (Rosenblatt et al, 1995, Punthuprapasa et al, 2001, Haghighi et al, 2005) Dot ELISA for serodiagnosis of amoebiasis is also reported (Mahajan et al, 1989: Lopez et al, 1991, Yamura et al, 2003, Van Doorn et al, 2005, Dhanalakshmi et al, 2016. The present study was undertaken to develop Intact Parasite Antigen-ELISA (IPA-ELISA)test in the diagnosis of intestinal and extraintestinal amoebiasis and also to determine its sensitivity and specificity in comparision to Plate-ELISA, Dot Elisa.The relative merits of tests were also found out for clinical and epidemiological sur-vey of amoebiasis.…”

Section: Introductionmentioning

confidence: 99%

Intact parasite-antigen ELISA test: a new dimension for serodiagnosis of Amoebiasis

Mishra

Sharma

Tewari

2018

JANS

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An Intact Parasite Antigen ELISA (IPA-ELISA) has been developed for detection of circulating antibodies against Entamoeba. histolytica. Axenically grown trophozoites of E.histolytica (NIH-200) after glutaraldehyde treatment, are used as Intact Parasite Antigen (IPA) as well biological active and imperishable base. Antigen over the surface of treated cells were allowed to interact with the antibody molecules of the test sera. The techniques of Plate, Dot-and IPA-ELISA are compared of their merits for clinical and epidemiological survey of amoebiasis. IPA-ELISA was found to be more sensitive (96.8%) and specific (92.3%) in detecting circulating antibodies compared to Plate-ELISA (sensitivity 90.5%, specificity 84.6%) and Do-ELISA (sensitivity 92.1%, specificity 86.1%). The entire IPA-ELISA test could be completed within 3 hours. Costwise IPA-ELISA is several times lesser than Dot-and Plate-ELISA. This test is most suitable for field and clinical trials for suspected cases of amoebiasis with no skilled hand required for diagnosis.

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Evaluation of an enzyme-linked immunoassay for the detection in serum of antibodies to Entamoeba histolytica

Cited by 10 publications

References 5 publications

Laboratory Diagnostic Techniques for Entamoeba Species

Laboratory Diagnostic Techniques for Entamoeba Species

Detection of serum antibody to Entameba histolytica in various population samples of amebic infection using an enzyme-linked immunosorbent assay

Intact parasite-antigen ELISA test: a new dimension for serodiagnosis of Amoebiasis

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