Detection of serum antibody to Entameba histolytica in various population samples of amebic infection using an enzyme-linked immunosorbent assay

Haghighi, Ali; Rezaeian, Mostafa

doi:10.1007/s00436-005-1418-3

Cited by 10 publications

(8 citation statements)

References 19 publications

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“…dispar. By use of an enzyme-linked immunosorbent assay-based test, it was more recently shown that just 8 of the 51 (15.6%) asymptomatic amebic cyst passers examined had anti-E. histolytica antibodies, supporting the hypothesis that E. dispar is more prevalent in asymptomatic cyst passers in this country (7).…”

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confidence: 80%

Comparison of a Stool Antigen Detection Kit and PCR for Diagnosis of Entamoeba histolytica and Entamoeba dispar Infections in Asymptomatic Cyst Passers in Iran

et al. 2006

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The present study was conducted to compare stool antigen detection with PCR for the diagnosis of Entamoeba sp. infection in asymptomatic cyst passers from Iran. Entamoeba dispar and, in one case, E. moshkovskii were the Entamoeba spp. found in the amebic cyst passers. There was a 100% correlation between the results from the TechLab E. histolytica II stool antigen kit and those from nested PCR. We concluded that E. dispar is much more common in asymptomatic cyst passers in Iran and that antigen detection and PCR are comparable diagnostic modalities.Entamoeba histolytica, E. dispar, and E. moshkovskii are morphologically identical but biochemically and genetically different. These parasites colonize the human gut, but only E. histolytica is thought to be capable of causing disease. Amebiasis, defined as asymptomatic, invasive intestinal or extraintestinal disease due to E. histolytica infection, is one of the most common parasitic infections worldwide, infecting about 50 million people, frequently in developing countries, resulting in 40,000 to 100,000 deaths per annum (21). Asymptomatic cyst passage is the most frequent manifestation of intestinal Entamoeba infection. All E. dispar and E. moshkovskii infections and 90% of E. histolytica infections are asymptomatic (21). The studies conducted to date that have used methods capable of differentiating between the two species suggest that, in general, E. dispar is much more prevalent than E. histolytica (9,10,11) and that only a small proportion of individuals specifically infected with E. histolytica will progress to having amebic disease (8). The necessity to identify and treat asymptomatic carriers of E. histolytica is emphasized by the observation that 10% of them develop invasive amebiasis in due course (6). Additionally, asymptomatic carriers are more likely to spread the disease than symptomatic persons with invasive diseases, as the latter individuals seek medical attention (12).In nearly all of the studies of asymptomatic amebic cyst passers in which the distribution of the two species has been investigated (1,3,5,11,16,18), E. dispar has been found to be much more prevalent than E. histolytica; however, in a few studies, including two from Mexico (14, 15) and one targeting mentally retarded individuals in Japan (20), either E. histolytica occult infections were more common than E. dispar infections or E. histolytica was the sole Entamoeba sp. found.Several microscopy-based epidemiological studies in Iran have shown Entamoeba sp. infection rates of 2.2% to 30% (13, 17). The current study, therefore, was designed to address the distribution of E. histolytica and E. dispar in regions of Iran where previous microscopy-based studies showed a high prevalence of infection and to compare the commercially available TechLab E. histolytica II kit to PCR.A total of 1,037 single fresh stool samples were collected from apparently healthy persons in rural and semirural settings of western (Luristan), northwestern (West Azerbaijan), and northeastern (Golestan) Iran, where...

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confidence: 80%

Comparison of a Stool Antigen Detection Kit and PCR for Diagnosis of Entamoeba histolytica and Entamoeba dispar Infections in Asymptomatic Cyst Passers in Iran

et al. 2006

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“…A partir del crecimiento que presentaron los hibridomas en las placas después de la clonación y reclonación se seleccionó el clon 3G8C3 secretor del anticuerpo monoclonal anti EhCP5r para la expansión in vitro e in vivo, (20)(21)(22)(23)(24).…”

Section: Resultsunclassified

Obtención de anticuerpos monoclonales de ratón contra proteasa de cisteína 5 recombinante de Entamoeba histolytica

2012

Rev MVZ Córdoba

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RESUMENObjetivo. Obtener anticuerpos monoclonales de ratón contra la proteasas de cisteína 5 (EhCP5) de Entamoeba histolytica. Materiales y métodos. Se inmunizaron ratones BALB/c por vía intraperitoneal con adyuvante de Freund completo e incompleto con la proteína recombinante EhCP5 obtenida a partir del cultivo de E.coli DH5α trasfectada con el vector recombinante pJC45 que expresa dicha proteína. Se seleccionó el animal con mejor respuesta de anticuerpos. Al cual se le extrajo su bazo como fuente de linfocitos B, los cuales se fusionaron utilizando PEG con células de mieloma de ratón SP2-0/Ag14. Se procedió a selección de los hibridomas y a la evaluación de los sobrenadantes de las colonias que crecieron a los 7 días mediante ELISA. Los hibridomas con valores más altos de anticuerpos específicos contra la proteína EhCP5r se seleccionaron, y los clones obtenidos por diluciones limitantes fueron expandidos. Resultados. A partir de un clon secretor estable se purifico el anticuerpo monoclonal anti EhCP5r del isotipo IgG1 por cromatografía de afinidad con proteína G. Los clones fueron expandidos in vivo e in vitro. Con el anticuerpo purificado se diseñaron tres sistemas de captura para evaluar la aplicabilidad del anticuerpo monoclonal anti EhCP5r como método inmunodiagnóstico. Conclusiones. Se logro la producción de un anticuerpo monoclonal específico contra EhCP5r que permite diferenciar Entamoeba histolytica de Entamoeba dispar.Palabras clave: Anticuerpo, Entamoeba histolytica, , hibridoma, proteasas de cisteína, monoclonal, prueba ELISA (Fuentes:CAB, DeCS). ABSTRACTObjective. Obtain mouse monoclonal antibodies against cysteine proteases 5 (EhCP5) of Entamoeba histolytica. Materials and methods. BALB/c mice were immunized intraperitoneally with complete and incomplete Freund adjuvant EhCP5 with the recombinant EhCP5 protein obtained from E.coli DH5α culture transfected with the recombinant vector pJC45 that expresses said protein. The animal with the best antibody response was selected. Its spleen was extracted as a source of B-lymphocytes, which were merged using PEG miceSP2-0/Ag14 myeloma cells. The team proceeded to undergo the selection of the hybridomas and the evaluation of the supernatants of the colonies that grew after 7 ORIGINALRev.MVZ Córdoba 17(2): 3014-3023, 2012.

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“…E. dispar has been shown to be 14.5 times more common than E. histolytica by the ELISA method, the only method that can definitively differentiate E. histolytica and E. dispar [7,24]. The more recent method of the detection of fecal antigens by ELISA is an alternative to microscopy, and the gold standard, with sensitivity and specificity over 95% [24,25]. In Sweden, 165 of 207 patients microscopically diagnosed with E. histolytica/E.…”

Section: Discussionmentioning

confidence: 98%

Prevalence and Importance of Amebic Infestation in Patients with Ulcerative Colitis in Two Regions in Turkey

et al. 2008

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We investigated the prevalence of amebiasis in patients with ulcerative colitis residing in two geographical regions with different socioeconomic status and climatic conditions, and its effect on the age of onset, duration, localization, and activity of disease. Ninety patients from a high socioeconomic location (group I) and 28 cases from a low socioeconomic location (group II) were enrolled. Median age at disease onset was significantly higher in group I compared with in group II. Prevalence of amebiasis in group I was significantly lower than in group II. A considerably number of patients with amebiasis in group I had a history of travel to the cities with a lower socioeconomic level, mainly located in the east of Turkey. There was a strong relationship between presence of amebiasis and history of travel to eastern parts of Turkey among residents from the northwestern part of Turkey. Median age and age at time of diagnosis were significantly lower in patients with amebiasis compared with those without infection. In patients with mild disease activity, prevalence of amebiasis was significantly lower compared with those with moderate or severe disease activity. In conclusion, prevalence of amebiasis was markedly higher in the southeast compared to the northwest of Turkey. Travel to regions with low socioeconomic status may be considered a risk factor for amebiasis in patients with ulcerative colitis. Amebiasis enhances disease activity in ulcerative colitis.

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Detection of serum antibody to Entameba histolytica in various population samples of amebic infection using an enzyme-linked immunosorbent assay

Cited by 10 publications

References 19 publications

Comparison of a Stool Antigen Detection Kit and PCR for Diagnosis of Entamoeba histolytica and Entamoeba dispar Infections in Asymptomatic Cyst Passers in Iran

Comparison of a Stool Antigen Detection Kit and PCR for Diagnosis of Entamoeba histolytica and Entamoeba dispar Infections in Asymptomatic Cyst Passers in Iran

Obtención de anticuerpos monoclonales de ratón contra proteasa de cisteína 5 recombinante de Entamoeba histolytica

Prevalence and Importance of Amebic Infestation in Patients with Ulcerative Colitis in Two Regions in Turkey

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