Evaluation of an automated system for the collection of packed RBCs, platelets, and plasma

Elfath, M. Dean; Whitley, Pamela; Jacobson, May S.; Cranfill‐Hupp, Kathy; Kemp, Donna M.; McNeil, Deanna; Sawyer, Sherrie; Bell‐Dize, Sharon; Gorlin, Jed B.; Kevy, Sherwin V.; McAteer, Michael J.

doi:10.1046/j.1537-2995.2000.40101214.x

Cited by 44 publications

(39 citation statements)

References 29 publications

(30 reference statements)

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“…The 24-hour RBC recovery determined by FC was 78.0 ± 12.0% (mean ± SD), 78.0 ± 15.0% when labeled RBCs originated from donor and 78.6 ± 11.6% when labeled RBCs originated from the recipient. These data are comparable to those published by Elfath et al [26] who measured a 24-hour RBC recovery of 76.8 ± 3.1% for RBC units collected with automated apheresis and a recovery of 77.1 ± 4.4% for whole-blood RBCs using 51 CR. However, it is not clear if the FDA criteria of more than 75% recovery for RBC units would have been met in our study if all RBCC were transfused at the end of the storage period.…”

Section: ----------------------------------------------------------supporting

confidence: 90%

Flow-Cytometric Determination of Survival Time and 24-Hour Recovery of Transfused Red Blood Cells

Zeiler¹,

Müller²,

Kretschmer³

2003

Transfus Med Hemother

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Introduction: We tested the feasibility of the determination of 24-hour recovery and survival time of transfused red blood cells (RBCs) by flow cytometry to evaluate a new RBC preparation in a clinical application study. Patients and Methods: Eleven patients received 22 inline filtered RBC concentrates from apheresis (mean storage time of transfused RBCs 25.6 ± 12 days). Recovery and survival were calculated based on flow-cytometric determination of autologous and transfused RBCs by measuring antigens differing between donor and recipient. Human polyclonal erythrocyte antibodies (IgG) were used as primary and FITC-conjugated rabbit antihuman IgG antibody (F(ab’)₂) fragments as secondary antibodies. Results: The mean (± SD), 24-hour recovery determined, using anti-c, anti-C, anti-D, anti-Jk^a and anti-Fy^a as primary antibodies, was 78 ± 12%, the mean RBC survival time was 70.1 ± 26.5 days. Discussion: Flow cytometry using blood group antigen differences between donor and recipient appears to be suitable for the determination of RBC 24-hour recovery and survival time. This method requires no pretransfusional labeling of the RBCs, thus avoiding any bias by manipulation of the transfused RBCs. However, this method is applicable only to allogeneous transfusion.

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Section: ----------------------------------------------------------supporting

confidence: 90%

Flow-Cytometric Determination of Survival Time and 24-Hour Recovery of Transfused Red Blood Cells

Zeiler¹,

Müller²,

Kretschmer³

2003

Transfus Med Hemother

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“…The functional range of pH has long been recognized as an important factor for storage. Figure 3 summarizes platelet recoveries versus pH for several of the historical studies plus more recent studies conducted with platelets stored in ELP bags 6,21,22 . Low pH was observed in 1 of 24 components in this study.…”

Section: Discussionmentioning

confidence: 81%

Seven‐day storage of apheresis platelets: report of an in vitro study

Dumont

VandenBroeke

2003

Transfusion

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“…It has been previously demonstrated that different cell separators induce different degrees of PLT activation, depending on their collection modalities 7‐9 , 21,23‐25 . Plateletpheresis, obtained using the surge technology, like those obtained with the Haemonetics MCS device, show lower levels of P‐selectin when compared to those obtained using Cobe Spectra or CS‐3000 machines 21 .…”

Section: Discussionmentioning

confidence: 99%

“…Multicomponent collection, which is the contemporary yield of RBCs and plasma, or plasma and PLTs, or RBCs and PLTs, has been recently introduced into blood collection center practices. This new donor apheresis technique, mostly performed through single‐vein access, allows the production of a leukoreduced plateletpheresis containing more than 3 × 10 11 PLTs in a consistent proportion of units (>75%) and the contemporary collection of a subsidiary 200‐ to 400‐mL plasma unit in 45 to 60 minutes 7‐9 …”

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confidence: 99%

Platelet activation during plasma‐reduced multicomponent PLT collection: a comparison between COBE Trima and Spectra LRS turbo cell separators

et al. 2004

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BACKGROUND: The wide diffusion of multicomponent collection in donor apheresis has led to the yielding of different components, such as plasma‐reduced platelet‐pheresis at high PLT concentration. We investigated whether this collection modality could induce more PLT activation compared to standard plateletpheresis. STUDY DESIGN AND METHODS: Forty‐one plateletpheresis collections (20 Trima and 21 Spectra LRS Turbo v.7.0, COBE) were evaluated. Donor, procedure, and product data were recorded. ADP, collagen, and U46619 (a thromboxane‐A2 analog)‐induced PLT aggregation was investigated in basal (donor) and final (plateletpheresis unit) samples. The expression of PLT activation marker P‐selectin (CD62P) was studied using flow cytometry in basal and final samples. In all cases, P‐selectin was investigated in final samples after stimulation with ADP to assess for a possible further release of the antigen. Four additional plateletpheresis procedures were performed in donors from Group A, using the traditional, nonplasma‐reduced program. RESULTS: Plateletpheresis obtained by means of the Trima device showed a lower response to in‐vitro induced PLT aggregation and a higher percentage of P‐selectin‐expressing PLT when compared to products obtained using the Spectra device. Moreover, P‐selectin release after ADP stimulation was reduced in plateletpheresis units obtained using the Trima device. These differences disappeared when a nonplasma‐reduced collection program was used. In‐vivo evaluation did not detect any difference between platelepheresis obtained by means of the two cell separators. CONCLUSIONS: Plateletpheresis units obtained by means of multicomponent collection show a higher degree of PLT activation compared to traditional plateletpheresis procedures when high‐concentration plasma‐reduced products are collected. Randomized clinical studies are needed to assess the real impact of these findings in terms of in‐vivo efficacy of plasma‐reduced plateletpheresis units.

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Evaluation of an automated system for the collection of packed RBCs, platelets, and plasma

Cited by 44 publications

References 29 publications

Flow-Cytometric Determination of Survival Time and 24-Hour Recovery of Transfused Red Blood Cells

Flow-Cytometric Determination of Survival Time and 24-Hour Recovery of Transfused Red Blood Cells

Seven‐day storage of apheresis platelets: report of an in vitro study

Platelet activation during plasma‐reduced multicomponent PLT collection: a comparison between COBE Trima and Spectra LRS turbo cell separators

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