Evaluation of allergenicity of cow milk treated with enzymatic hydrolysis through a mouse model of allergy

Liang, Xiaona; Wang, Zongzhou; Yang, Hui; Luo, Xue; Sun, Jing; Yang, Mei; Shi, Xinyang; Yue, Xiqing; Zheng, Yan

doi:10.3168/jds.2021-20686

Cited by 26 publications

(22 citation statements)

References 41 publications

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“…Enzymatic hydrolysis has received extensive attention owing to its high efficiency, selectivity, reproducibility, and environmental friendliness, and is considered to be an effective method to reduce food allergic reactions. , Liang et al found that the binding levels of IgE in milk were reduced by 56.31, 50.62, and 56.45% after enzymatic hydrolysis by alcalase, protamex, and flavorzyme, respectively, which could significantly inhibit the allergic reactions of milk . Xue et al observed that hydrolysis by alcalase and papain significantly reduced the levels of gliadin-specific IgE, histamine, and serum cytokines .…”

Section: Introductionmentioning

confidence: 99%

Pulsed Electric Field-Assisted Alcalase Treatment Reduces the Allergenicity and Eliminates the Antigenic Epitopes of Ovomucoid

Ding

Zhang

et al. 2023

J. Agric. Food Chem.

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Physically assisted chemical modifications can effectively reduce the allergenicity of ovomucoid (OVM). However, only a few studies have used pulsed electric field (PEF)-assisted alcalase hydrolysis to reduce the allergenicity of OVM. Herein, we investigated the effect of PEF-assisted alcalase treatment on the spatial conformation, allergenicity, and antigenic epitopes of OVM based on multispectroscopic analyses, bioinformatics, and mass spectrometry. The results showed that PEF-assisted alcalase treatment promoted the hydrolysis of OVM; moreover, the α-helix content and surface hydrophobicity of OVM significantly decreased, which disordered its spatial conformation and weakened its intermolecular interactions. Additionally, enzyme-linked immunosorbent assay (ELISA) results showed that the PEF-assisted alcalase treatment significantly reduced the binding levels of IgE and IgG1, which were 47.66 and 36.41%, respectively. Finally, eight epitopes of OVM were obtained by immunoinformatic tools. Nano-high performance liquid chromatography coupled to tandem mass spectrometry (nano-HPLC MS/MS) results showed that the hydrolysate of OVM and alcalase (HOVM) had nine more peptide-containing epitopes than the hydrolysate of PEF-treated OVM and PEF-treated alcalase (HOVM-PP′), indicating that PEF could promote the elimination of linear epitopes in OVM, thereby reducing OVM allergenicity.

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Section: Introductionmentioning

confidence: 99%

Pulsed Electric Field-Assisted Alcalase Treatment Reduces the Allergenicity and Eliminates the Antigenic Epitopes of Ovomucoid

Ding

Zhang

et al. 2023

J. Agric. Food Chem.

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“…Moreover, there are animal models established for the evaluation of inherent immunogenicity and allergenicity of infant formulas as well as for the assessment of their preventive capacity [ 148 , 149 , 150 , 151 , 152 ]. Several animal studies have shown reduced allergenicity of pHF [ 153 , 154 , 155 ]. In one study, it was shown that pHFs did not induce sensitisation [ 155 ], while another study showed induction of sensitisation but without clinical symptom manifestation [ 156 ].…”

Section: Cow’s Milk-based Infant Formulasmentioning

confidence: 99%

Alternatives to Cow’s Milk-Based Infant Formulas in the Prevention and Management of Cow’s Milk Allergy

Maryniak

Sancho

Hansen

et al. 2022

Foods

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Cow’s milk-based infant formulas are the most common substitute to mother’s milk in infancy when breastfeeding is impossible or insufficient, as cow’s milk is a globally available source of mammalian proteins with high nutritional value. However, cow’s milk allergy (CMA) is the most prevalent type of food allergy among infants, affecting up to 3.8% of small children. Hypoallergenic infant formulas based on hydrolysed cow’s milk proteins are commercially available for the management of CMA. Yet, there is a growing demand for more options for infant feeding, both in general but especially for the prevention and management of CMA. Milk from other mammalian sources than the cow, such as goat, sheep, camel, donkey, and horse, has received some attention in the last decade due to the different protein composition profile and protein amino acid sequences, resulting in a potentially low cross-reactivity with cow’s milk proteins. Recently, proteins from plant sources, such as potato, lentil, chickpeas, quinoa, in addition to soy and rice, have gained increased interest due to their climate friendly and vegan status as well as potential lower allergenicity. In this review, we provide an overview of current and potential future infant formulas and their relevance in CMA prevention and management.

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“…In addition, since some newborns are severely allergic to cow's milk, the macromolecular proteins in cow's milk are degraded into short peptides and amino acids through hydrolysis technology to eliminate allergenic proteins (12,13). Currently, infant formulas on the market include partially hydrolyzed infant formula (pHIF) and extensively hydrolyzed infant formula (eHIF); the former can be used to prevent cow's milk protein allergy, and the latter can be given to infants with cow's milk allergy (1).…”

Section: Introductionmentioning

confidence: 99%

In vitro Digestion Characteristics of Hydrolyzed Infant Formula and Its Effects on the Growth and Development in Mice

Feng

Zhang

et al. 2022

Front. Nutr.

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Infant formula, an important food for babies, is convenient and nutritious, and hydrolyzed formulas have attracted much attention due to their non-allergicity. However, it is uncertain whether hydrolyzed formulars cause obesity and other side effects in infants. Herein, three infant formulas, standard (sIF), partially hydrolyzed (pHIF), and extensively hydrolyzed (eHIF), were analyzed in an in vitro gastrointestinal digestion model. With increasing degree of hydrolysis, the protein moleculars, and allergenicity of the proteins decreased and the long-chain polyunsaturated fatty acid content increased. Moreover, the digestion model solutions quickly digested the small fat globules and proteins in the hydrolyzed formula, allowing it to become electrostatically stable sooner. The eHIF-fed mice presented larger body sizes, and exhibited excellent exploratory and spatial memory abilities in the maze test. Based on villus height and crypt depth histological characterizations and amplicon sequencing, eHIF promoted mouse small intestine development and changed the gut microbiota composition, eventually favoring weight gain. The mouse spleen index showed that long-term infant formula consumption might be detrimental to immune system development, and the weight-bearing swimming test showed that eHIF could cause severe physical strength decline. Therefore, long-term consumption of infant formula, especially eHIF, may have both positive and negative effects on mouse growth and development, and our results might shed light on feeding formula to infants.

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Evaluation of allergenicity of cow milk treated with enzymatic hydrolysis through a mouse model of allergy

Cited by 26 publications

References 41 publications

Pulsed Electric Field-Assisted Alcalase Treatment Reduces the Allergenicity and Eliminates the Antigenic Epitopes of Ovomucoid

Pulsed Electric Field-Assisted Alcalase Treatment Reduces the Allergenicity and Eliminates the Antigenic Epitopes of Ovomucoid

Alternatives to Cow’s Milk-Based Infant Formulas in the Prevention and Management of Cow’s Milk Allergy

In vitro Digestion Characteristics of Hydrolyzed Infant Formula and Its Effects on the Growth and Development in Mice

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