Evaluation of acute toxicity and gastroprotective activity of curcuma purpurascens BI. rhizome against ethanol-induced gastric mucosal injury in rats

Rouhollahi, Elham; Moghadamtousi, Soheil Zorofchian; Hamdi, Omer Abdalla Ahmed; Fadaeinasab, Mehran; Hajrezaie, Maryam; Awang, Khalijah; Looi, Chung Yeng; Abdulla, Mahmood Ameen; Mohamed, Zahurin

doi:10.1186/1472-6882-14-378

Cited by 36 publications

(39 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Additionally, no differences in body weights were observed for both sex SD rats. These findings corroborate previous studies [18–20]. Our data suggest that C. barometz hair extract has high free-radicals scavengings and antioxidant activities in vitro.…”

Section: Discussionsupporting

confidence: 93%

“…However, the down-regulation of Heat shock protein expression appeared in the ulcer control animals as a gastric injury marker. This is consistent with the results from other studies [20, 46]. Up-regulation of HSP70 protein in the stomach wall mucosa in animals pre-fed with C. barometz hair extract may stop the initiation of damage to the gastric epithelium upon exposure to absolute alcohol [23, 32].…”

Section: Discussionsupporting

confidence: 92%

See 1 more Smart Citation

RETRACTED ARTICLE: The gastro protective effects of Cibotium barometz hair on ethanol-induced gastric ulcer in Sprague-Dawley rats

et al. 2017

Self Cite

View full text Add to dashboard Cite

Background Cibotium barometz is a medical herb used traditionally in the Malaysian peninsula for several ailments, including gastric ulcer. The aim of this study was assessment the anti-ulcer effects of C. barometz hair on ethanol-induced stomach hemorrhagic abrasions in animals. Seven groups of Sprague Dawley (SD) rats were administered 10% Tween 20 in the normal control and ulcer control groups, and omeprazole 20 mg/kg and 62.5, 125, 250, and 500 mg/kg of C. barometz hair extract in the experimental groups. After 60 min, the normal control group of rats was orally administered 10% Tween 20, while absolute ethanol was orally administered to the groups of ulcer control, omeprazole and experimental groups. Stomachs of the rats were examined macroscopically and histologically. Homogenates of stomachs were used to evaluate endogenous antioxidant enzyme activities.ResultsRats pre-fed with plant extract presented a significant decrease in the sore area, increased pH of gastric contents and preserved stomach wall mucus compared to the ulcer group. Histologically, rats pre-fed with C. barometz hair extract showed mild to moderate disruptions of the surface epithelium while animals pre-fed with absolute ethanol showed severe disruptions of the stomach epithelium with edema and leucocyte penetration of the submucosal layer. A Periodic acid Schiff (PAS) staining revealed that each rat pre-treated with the plant extract displayed an intense uptake of stomach epithelial glycoprotein magenta color compared to the ulcer control group. Immunohistochemical analysis revealed that rats pre-fed with the plant extract showed an up-regulation of the heat shock protein 70 (HSP70) and down-regulation of Bax proteins compared to ulcer control rats. Homogenates of the stomach tissue demonstrated significant increases in the endogenous antioxidant enzymatic activity and decreased lipid peroxidation (MDA) in rats pre-treated with C. barometz hair extract compared with the ulcer control rats. In acute toxicity, the liver and kidney revealed no hepatotoxic or nephrotoxic effects histologically.ConclusionsThe gastric cytoprotective action of C. barometz hair extract might be attributed to antioxidants, an increase in gastric pH, stomach mucus preservation, increased endogenous antioxidant enzymes, decreased lipid peroxidation, up-regulation of HSP70 and down-regulation of Bax proteins.

show abstract

Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 92%

RETRACTED ARTICLE: The gastro protective effects of Cibotium barometz hair on ethanol-induced gastric ulcer in Sprague-Dawley rats

et al. 2017

Self Cite

View full text Add to dashboard Cite

show abstract

“…The data established that C. barometz extract has potent antioxidant activities and free radical scavenging effects in vitro. Similarly, the herbal medicine has antioxidant efficiency and causes neutralization of free radicals as reported by several co-researchers 38–41…”

Section: Discussionmentioning

confidence: 78%

The antiulcer effect of <em>Cibotium barometz</em> leaves in rats with experimentally induced acute gastric ulcer

Al-Wajeeh¹,

Hajrezaie²,

Al-Henhena³

et al. 2017

DDDT

Self Cite

View full text Add to dashboard Cite

Cibotium barometz is a pharmaceutical plant customarily used in traditional medicine in Malaysia for the treatment of different diseases, such as gastric ulcer. The gastroprotective effect of C. barometz leaves against ethanol-induced gastric hemorrhagic abrasions in Sprague Dawley rats has been evaluated in terms of medicinal properties. Seven groups of rats (normal control and ulcerated control groups, omeprazole 20 mg/kg, 62.5, 125, 250, and 500 mg/kg of C. barometz correspondingly) were used in antiulcer experiment and pretreated with 10% Tween 20. After 1 hour, the normal group was orally administered 10% Tween 20, whereas absolute alcohol was fed orally to ulcerated control, omeprazole, and experimental groups. Gastric’s homogenate were assessed for endogenous enzymes activities. Stomachs were examined macroscopically and histologically. Grossly, the data demonstrated a significant decrease in the ulcer area of rats pretreated with plant extract in a dose-dependent manner with respect to the ulcerated group. Homogenates of the gastric tissue exhibited significantly increased endogenous enzymes activities in rats pretreated with C. barometz extract associated with the ulcerated control group. Histology of rats pretreated with C. barometz extract group using hematoxylin and eosin staining exhibited a moderate-to-mild disruption of the surface epithelium with reduction in submucosal edema and leucocyte infiltration in a dose-dependent manner. In addition, it showed heat shock protein70 protein up-expression and BCL2-associated X protein downexpression. These outcomes might be attributed to the gastroprotective and antioxidative effects of the plant.

show abstract

“…Our data found that LMCS significantly inhibited MPO activity and restored it to normal levels from 7 day, which was earlier than OMZ form 14 day. The reason for this discrepancy could be related to the good antiinflammatory of chitosan and sepia ink 18 .…”

Section: Discussionmentioning

confidence: 99%

“…The mechanisms of ethanol-induced gastric ulcer mainly includes breaking the mucosal barrier, back diffusion of acid, increased gastric mucosal permeability and depletion of certain oxygen free radical scavengers 18 . Also, metabolism of ethanol also releases free radicals 19 .…”

Section: Discussionmentioning

confidence: 99%

Therapeutic effect of low molecular weight chitosan containing sepia ink on ethanol-induced gastric ulcer in rats

Zhang

Liu

et al. 2016

Acta Cir. Bras.

View full text Add to dashboard Cite

PURPOSE:To evaluate the role of low molecular chitosan containing sepia ink (LMCS) in ethanol-induced (5 ml/kg) gastric ulcer in rats. METHODS:Animals were divided into four groups (n = 12): normal group (Normal), negative control group (Con), experiment group (LMCS) and positive control Omeprazole group (OMZ). Gastric empty rate was detected in the first 7 days. Rats were sacrificed at 7, 14 and 21 day for histology and ELISA detections. RESULTS:Gastric empty was no significant differences among the groups (P > 0.05). Histological observation showed gastric mucosal LMCS treated had better healing effect. Hydroxyproline (Hyp) was significantly increased from 7 day (P < 0.05). LMCS significantly inhibited malondialdehyde (MDA) generation for lipid peroxidation from 7 day (P < 0.05). LMCS significantly promoted the activity of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) at the earlier stage (P < 0.05). OMZ had the similar effects above. As for myeloperoxidase (MPO), LMCS significantly decreased and restored it to normal levels from 7 day (P < 0.05), it is earlier than OMZ which is from 14 day.CONCLUSION: LMCS can improve gastric mucosa tissue repair, exert significant influences on oxidative and antioxidant enzyme activities and neutrophil infiltration.

show abstract

Evaluation of acute toxicity and gastroprotective activity of curcuma purpurascens BI. rhizome against ethanol-induced gastric mucosal injury in rats

Cited by 36 publications

References 47 publications

RETRACTED ARTICLE: The gastro protective effects of Cibotium barometz hair on ethanol-induced gastric ulcer in Sprague-Dawley rats

RETRACTED ARTICLE: The gastro protective effects of Cibotium barometz hair on ethanol-induced gastric ulcer in Sprague-Dawley rats

The antiulcer effect of <em>Cibotium barometz</em> leaves in rats with experimentally induced acute gastric ulcer

Therapeutic effect of low molecular weight chitosan containing sepia ink on ethanol-induced gastric ulcer in rats

Contact Info

Product

Resources

About