RETRACTED ARTICLE: The gastro protective effects of Cibotium barometz hair on ethanol-induced gastric ulcer in Sprague-Dawley rats

Abstract

Background Cibotium barometz is a medical herb used traditionally in the Malaysian peninsula for several ailments, including gastric ulcer. The aim of this study was assessment the anti-ulcer effects of C. barometz hair on ethanol-induced stomach hemorrhagic abrasions in animals. Seven groups of Sprague Dawley (SD) rats were administered 10% Tween 20 in the normal control and ulcer control groups, and omeprazole 20 mg/kg and 62.5, 125, 250, and 500 mg/kg of C. barometz hair extract in the experimental groups. After 60 min, the normal control group of rats was orally administered 10% Tween 20, while absolute ethanol was orally administered to the groups of ulcer control, omeprazole and experimental groups. Stomachs of the rats were examined macroscopically and histologically. Homogenates of stomachs were used to evaluate endogenous antioxidant enzyme activities.ResultsRats pre-fed with plant extract presented a significant decrease in the sore area, increased pH of gastric contents and preserved stomach wall mucus compared to the ulcer group. Histologically, rats pre-fed with C. barometz hair extract showed mild to moderate disruptions of the surface epithelium while animals pre-fed with absolute ethanol showed severe disruptions of the stomach epithelium with edema and leucocyte penetration of the submucosal layer. A Periodic acid Schiff (PAS) staining revealed that each rat pre-treated with the plant extract displayed an intense uptake of stomach epithelial glycoprotein magenta color compared to the ulcer control group. Immunohistochemical analysis revealed that rats pre-fed with the plant extract showed an up-regulation of the heat shock protein 70 (HSP70) and down-regulation of Bax proteins compared to ulcer control rats. Homogenates of the stomach tissue demonstrated significant increases in the endogenous antioxidant enzymatic activity and decreased lipid peroxidation (MDA) in rats pre-treated with C. barometz hair extract compared with the ulcer control rats. In acute toxicity, the liver and kidney revealed no hepatotoxic or nephrotoxic effects histologically.ConclusionsThe gastric cytoprotective action of C. barometz hair extract might be attributed to antioxidants, an increase in gastric pH, stomach mucus preservation, increased endogenous antioxidant enzymes, decreased lipid peroxidation, up-regulation of HSP70 and down-regulation of Bax proteins.

Comparative study of the therapeutic effect of pomegranate alone or in combination with bone marrow mesenchymal stem cells on experimentally induced gastric ulcer in adult male rats: A histological and immunohistochemical study

“…Group II of our study revealed significant decrease of PAS positive reaction in fundic mucosal cells, consistent with some researches [3,7,33,34] , whereas ethanol-induced ulcer was associated with decrease of PAS positive reaction in fundic mucosal cells as result of ethanol damaging effect on the mucus cells or excessive oxidative stress. Moreover, there was a significant decrease of expression VEGF immunostaining in gastric mucosa, similar to some previous histopathological studies [35,36] .…”

“…They include gastric and duodenal ulcer. Gastric ulcer is an interruption of the continuity of the gastric or duodenal mucosa that extends towards the muscularis mucosa and may reach deeper into submucosa [1][2][3] . It can cause major complications such as bleeding, perforation and finally death if associated with decompensating of coexisting medical conditions [1,4] .…”

“…However, all the current therapies are not always effective, have adverse effects, and are expensive with high recurrence rate of ulcer. For this reason, there is a need to search for alternative drug sources or natural products which are more effective and safe against gastric ulcers [3,7] . Nowadays, dietary antioxidants of natural products are considered as promising alternative therapy and may have several health benefits [1] .…”

Gastroprotective Effect of Punicalagin against Ethanol-Induced Gastric Ulcer: The Possible Underlying Mechanisms

“…The destructive factors include stomach Hydrochloric Acid (HCl), free oxygen radicals, ethanol, Helicobacter pylori and Non-Steroidal Anti-Inflammatory Drugs (NSAIDs) that encourage the gastric mucosal injury leading to gastric ulceration [1]. Alcohol consumption has been commonly related to mucosal inflammation, gastric ulcer and even gastric carcinoma [2].…”

Investigation of Toxicity and Systemic Exposure of Bioactive Compounds of Aqueous Leaf Extract of <i>Azadirachta indica</i> A. Juss (<i>Meliaceae</i>) on Wistar Rats

“…Medicinal plants (plants which have been used for medical purposes at one time or another, and which, although not necessarily a product available for marketing, is the original material of herbal medicines [21][22][23]) long played important roles in the treatment of diseases all over the world. Healing with medicinal plants is as old as mankind itself.…”