Evaluation of acute and subacute toxicity of aqueous extract of Crassocephalum rubens leaves in rats

Adewale, Olusola Bolaji; Onasanya, A.; Anadozie, Scholastica Onyebuchi; Abu, Miriam F.; Akintan, Idowu A.; Ogbole, Catherine J.; Olayide, Israel; Afolabi, Olakunle Bamikole; Jaiyesimi, Kikelomo Folake; Ajiboye, B.O.; Fadaka, Adewale Oluwaseun

doi:10.1016/j.jep.2016.05.003

Cited by 34 publications

(33 citation statements)

References 11 publications

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“…The reduction of ALT suggests that V. litoralis has some hepatoprotective properties that may be related to its chemical composition identified by UHPLC/MS, since luteolin, chlorogenic acid, caffeic acid and apigenin have already been proved to possess such activity. Corroborating with our findings, other studies have also reported similar hepatoprotective effects of plants (Brondani et al, 2017;Adewale et al, 2016;Pereira, Barros, Ferreira, 2016). of commercial kits for biochemical and hematological analyzes of blood.…”

Section: Discussionsupporting

confidence: 92%

Anti-inflammatory activity and identification of the Verbena litoralis Kunth crude extract constituents

Lima

Brondani

Dornelles

et al. 2020

Braz. J. Pharm. Sci.

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Verbena litoralis is a plant popularly known as "gervãozinho-do-campo" in Portuguese. It is traditionally used for stomach, liver and gallbladder problems, and as an anti-inflammatory and anthelmintic. The goal of this study was to determine the chemical composition of the crude extract obtained from the aerial parts of V. litoralis by Ultra High Performance Liquid chromatography coupled with Mass Spectrometry in Tandem (UHPLC-MS/MS); assess the anti-inflammatory activity of ethyl acetate fraction and of crude extract; and verify liver, kidney and pancreas damage. In this study, the chemical composition of the extract was identified via UHPLC/MS/MS, assessing the anti-inflammatory activity of the crude extract and the acetate fraction in an induction model of the granulomatous tissue, as well as given liver, kidney and pancreas damage markers. Chlorogenic acid, luteolin, caffeic acid, apigenin, p-coumaric acid, vanillic acid, ferulic acid and quercetin were quantified in the extract. After the seven-day treatment, the granuloma of the animals treated with the plant extract and fraction presented values very close to the positive control (nimesulide). The V. litoralis crude extract and ethyl acetate fraction show antiinflammatory activity similar to the nimesulide without evidence of liver, kidney and pancreas damage, which attributes the plant's pharmacological action to the flavonoids found.

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Section: Discussionsupporting

confidence: 92%

Anti-inflammatory activity and identification of the Verbena litoralis Kunth crude extract constituents

Lima

Brondani

Dornelles

et al. 2020

Braz. J. Pharm. Sci.

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“…NERG treatment at 1,000 mg/ml showed significantly increased serum levels of albumin, calcium, cholesterol, HDL-cholesterol, and iron relative to control mice. The hematological analysis is important because the hematopoietic system is one of the most sensitive targets of toxic compounds (Adewale et al, 2016). LY and MO showed significantly decreased values in NERG-treated mice compared to the control group (Table 4).…”

Section: Discussionmentioning

confidence: 99%

Acute and subchronic toxicity study of nonpolar extract of licorice roots in mice

Kim

Zuo

Lee

et al. 2020

Food Science & Nutrition

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Licorice is used as a medicinal plant, and several studies have shown that licorice has beneficial effects. The objective of this study was to evaluate the safety of nonpolar licorice extract using toxicity experiments. Nonpolar extract from the root of Glycyrrhiza uralensis (NERG) was analyzed by high-performance liquid chromatography (HPLC). Antioxidant ability was determined by method of TPC and DPPH. Blood pressure was monitored by using blood pressure meter. In the acute study, a single dose (2,000 mg/kg) was orally administered to mice. In the subchronic study, mice were treated with extract at doses (50, 100, 500, and 1,000 mg/kg) for 120 days.Significantly difference was not shown at blood pressure, hematological, and biochemical parameters, and histopathology on mice. The results suggested that at acute and subchronic toxicity, each levels of nonpolar licorice extract administration in experiments did not cause toxicity effects or death on mice. K E Y W O R D S acute toxicity, de-glycyrrhizin, licorice, nonpolar extract, subchronic toxicity, toxicology | 2243 KIM et al. 2017). Since information about the toxicity of licorice extract is limited, it is important to conduct studies to assess the safety of nonpolar licorice root extract. Therefore, the objective of this study was to evaluate the safety of nonpolar licorice root extract in mice by utilizing acute and subchronic toxicity tests. Our aim is to provide valuable information regarding the usage of this extract. | MATERIAL S AND ME THODS | Plant material, preparation of extraction, and administration of extractThe root of licorice was obtained from Deagwang korean plant sales, Chuncheon, Korea. The specimen of G. uralensis was confirmed to Seoul national university and deposited in the Regional Innovation Center (RIC; #040322) of the Hallym University, Chuncheon.The plant root was air-dried and then soaked in a mixture of solvent (Hexane:Ethanol = 9:1) for 24 hr. After 24 hr, the extraction was filtered and concentrated at 37°C using a rotary evaporator (Vacuubrand, CVC 3000). The nonpolar extract from the root of G. uralensis (NERG) was administered orally, in the shape of a pellet, for the acute and subchronic toxicity studies. | Chemical and reagentsAll chemicals were analytical grade. Acetonitrile was acquired from J. T. Baker Chemical Company. Acetic acid, Folin-Ciocalteu phenol reagent, and 2,2-diphenyl-1-picrylhydrazyl (DPPH) were purchased from Sigma Chemical Company.

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“…In addition, impaired hepatic function and glucose metabolism may be a contributing mechanism for induced hypoglycemia [28]. Many others studies reported similar findings regarding hepatoprotective effect of plants [29,30].…”

Section: Hematologicalmentioning

confidence: 75%

Evaluation of Acute and Sub-acute Toxicity of the Aqueous Extract from the Fruit of Solanum indicum Linn. (Solanaceae) in Rats

Epoh

Dongmo

Tadjoua

et al. 2019

EJMP

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Aim: The fruit of Solanum indicum Linn have been reported traditionally to have anti-hypertensive and diuretic properties. This study was undertaken to evaluate the toxicological potential of S. indicum fruits aqueous extract through the acute and sub-acute toxicity tests in rats. Methodology: For acute toxicity evaluation, a single oral dose of 5000 mg/kg of the plant extract was administrated in 60 days old female albino Wistar rats. Then, the animals were observed for 14 days. Sub-acute toxicity studies were conducted with 50 adult rats of both gender that orally received during 28 days, increasing doses of the plant extract. Their body weight and food intake were weekly collected. At the end of the experiment, biochemical and hematological parameters as well as histological analysis of organs (liver, kidneys and spleen) were undertaken. Results: Single oral administration of 5000 mg/kg dose of the fruit plant aqueous extract produced no mortality or signs of toxicity. During sub-acute test, no variations in body weight and food intake of both animals gender were observed. An important decrease in male’s rat liver weight were obtained at the dose 25 mg/kg; serum urea, total cholesterol, TAG, ALP and AST levels were signiﬁcantly lowered in male especially at the dose 50 mg/kg, but this decrease was noticed only in serum urea, ALP and ALT in female rats. Furthermore, a significant decrease in platelets number, serum PCT, MPV and PDW levels were recorded in all treated male rats except those receiving the highest extract dose. No structural changes in treated animal organs section histology were observed when compared to controls. Conclusion: The fruits aqueous extracts of S. indicum is safe when administered acutely and for 28 days in rats. However, alterations on their hematological and biochemical parameters were not closely related with the dose, implying caution on its use.

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Evaluation of acute and subacute toxicity of aqueous extract of Crassocephalum rubens leaves in rats

Cited by 34 publications

References 11 publications

Anti-inflammatory activity and identification of the Verbena litoralis Kunth crude extract constituents

Anti-inflammatory activity and identification of the Verbena litoralis Kunth crude extract constituents

Acute and subchronic toxicity study of nonpolar extract of licorice roots in mice

Evaluation of Acute and Sub-acute Toxicity of the Aqueous Extract from the Fruit of Solanum indicum Linn. (Solanaceae) in Rats

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