Evaluation of a Screening Test for Detection of
            <i>Giardia</i>
            and
            <i>Cryptosporidium</i>
            Parasites

Youn, Sojin; Kabir, Mamun; Petri, William A.

doi:10.1128/jcm.01736-08

Cited by 33 publications

(21 citation statements)

References 33 publications

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“…In the same study, Remel ProSpect Giardia / Cryptosporidium and IVD Research Giardia / Cryptosporidium ELISA kits offered sensitivities of 91.4% and 92.8%, respectively. In many previous studies the kit proved to be 100% specific; however, in this study, it exhibited a slightly lower specificity of 95.71% [28, 29]. This reduced specificity of the TechLab ELISA kit could not be explained.…”

Section: Discussioncontrasting

confidence: 69%

Evaluation of an Immunoassay-Based Algorithm for Screening and Identification ofGiardiaandCryptosporidiumAntigens in Human Faecal Specimens from Saudi Arabia

Hawash

2014

Journal of Parasitology Research

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An immunoassay-based algorithm, involving three commercial kits, was introduced and evaluated for screening and identification of Giardia/Cryptosporidium antigens in human stool specimens. Initially, Giardia/Cryptosporidium Chek kit (TechLab), an enzyme-linked immunosorbent assay (ELISA), was adopted for screening. The ELISA-positive reactions were subsequently characterised by RIDA Quick Giardia and RIDA Quick Cryptosporidium immunochromatographic kits (R-Biopharm). A gold standard test comprising PCR and microscopy was used for preparing control samples. Performance of individual kits was tested against these samples which included 50 Giardia-positive, 40 Cryptosporidium-positive, and 70 Cryptosporidium/Giardia-negative. For Cryptosporidium, specificities of the ELISA and RIDA Quick Cryptosporidium kits were 95.71% and 100%, respectively. Both kits demonstrated sensitivity of 95%. For Giardia, the ELISA and RIDA Quick Giardia kits showed sensitivities of 100% and 97.5%, respectively. Specificities obtained by the ELISA and RIDA Quick Giardia were 95.7% and 100%, respectively. Based on the results of two reference PCRs, on 250 random samples, the algorithm exhibited sensitivity, specificity, positive predictive value, and negative predictive value of 97.06%, 100.00%, 100.00%, and 98.91%, respectively. In conclusion, this immunoassay-based algorithm can be used as routine test in diagnostic laboratories for screening and identification of a large number of samples.

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Section: Discussioncontrasting

confidence: 69%

Evaluation of an Immunoassay-Based Algorithm for Screening and Identification ofGiardiaandCryptosporidiumAntigens in Human Faecal Specimens from Saudi Arabia

Hawash

2014

Journal of Parasitology Research

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“…However, diagnostic sensitivities are variable (70% to 100%). 46–48,52 Some rapid tests have reduced specificity and sensitivity for species other than C parvum or C hominis , 53,49 and confirmation of positive reactions is needed. 49 …”

Section: Diagnosticsmentioning

confidence: 99%

A review of the global burden, novel diagnostics, therapeutics, and vaccine targets for cryptosporidium

Checkley

White

Jaganath

et al. 2015

The Lancet Infectious Diseases

802

703

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Cryptosporidium spp are well recognised as causes of diarrhoeal disease during waterborne epidemics and in immunocompromised hosts. Studies have also drawn attention to an underestimated global burden and suggest major gaps in optimum diagnosis, treatment, and immunisation. Cryptosporidiosis is increasingly identified as an important cause of morbidity and mortality worldwide. Studies in low-resource settings and high-income countries have confirmed the importance of cryptosporidium as a cause of diarrhoea and childhood malnutrition. Diagnostic tests for cryptosporidium infection are suboptimum, necessitating specialised tests that are often insensitive. Antigen-detection and PCR improve sensitivity, and multiplexed antigen detection and molecular assays are underused. Therapy has some effect in healthy hosts and no proven efficacy in patients with AIDS. Use of cryptosporidium genomes has helped to identify promising therapeutic targets, and drugs are in development, but methods to assess the efficacy in vitro and in animals are not well standardised. Partial immunity after exposure suggests the potential for successful vaccines, and several are in development; however, surrogates of protection are not well defined. Improved methods for propagation and genetic manipulation of the organism would be significant advances.

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“…'de omurgalıların bir bağırsak protozoonudur (6). İki protozoon oral-fekal yolla, kontamine su ve yiyeceklerin tüketilmesiyle bulaşmaktadır ve ishalin en önemli iki etkenidir (7)(8)(9)(10)(11).…”

Section: Introductionunclassified

Comparison of Direct Microcopy and Antigen Casette Tests for the Detection of Giardia and Cryptosporidium

Doni

Zeyrek²,

Gürses³

et al. 2013

TurkiyeParazitolDerg

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When we compared the results of the direct microscopy and antigen casette tests, we found no significant difference between them for test reliability (p > 0.05). Antigen tests have higher sensitivity (100%) and specifity (100%) than the modified acid-fast staining technique, therefore, it is a preferred reference method . However, an experienced staff working accurately might access the same conclusion. Considering the cost of antigen tests, direct microscopic examination is cheaper, andeasier when it used by an experienced person.

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Evaluation of a Screening Test for Detection of Giardia and Cryptosporidium Parasites

Cited by 33 publications

References 33 publications

Evaluation of an Immunoassay-Based Algorithm for Screening and Identification ofGiardiaandCryptosporidiumAntigens in Human Faecal Specimens from Saudi Arabia

Evaluation of an Immunoassay-Based Algorithm for Screening and Identification ofGiardiaandCryptosporidiumAntigens in Human Faecal Specimens from Saudi Arabia

A review of the global burden, novel diagnostics, therapeutics, and vaccine targets for cryptosporidium

Comparison of Direct Microcopy and Antigen Casette Tests for the Detection of Giardia and Cryptosporidium

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