Evaluation of a saline boiled extract, capsular polysaccharides and long-chain lipopolysaccharides of Actinobacillus pleuropneumoniae serotype 1 as antigens for the serodiagnosis of swine pleuropneumonia

Gottschalk, Marcelo; Altman, Eleonora; Charland, Nathalie; Lasalle, Francis de; Dubreuil, J. Daniel

doi:10.1016/0378-1135(94)90009-4

Cited by 33 publications

(33 citation statements)

References 25 publications

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“…4). This antigen most probably corresponds to the O-chain LPS, as previously shown by Gottschalk et al [4] for A. pleuropneumoniae. Hence, antigens detected by ELISA were present in both, the bacterin Figure 1.…”

Section: Serum Antibody Response To An Autogenous Vaccinesupporting

confidence: 79%

“…In the selected herd, respiratory problems and sudden deaths were occurring in 16 to 19-week-old pigs. The herd was known to be serologically negative for all serotypes of A. pleuropneumoniae as routinely tested by a recognized ELISA test [4]. The antibody response to an autogenous vaccine was evaluated in gilts newly introduced into a breeding herd.…”

Section: Animalsmentioning

confidence: 99%

“…An indirect ELISA was adapted and standardized for this study from a method described by Gottschalk et al [4]. Since preliminary results showed no significant serological differences when either of the two isolates were used for the antigen preparation (results not shown), the field isolate AS1 was chosen as the source for the ELISA antigen throughout this study.…”

Section: Elisamentioning

confidence: 99%

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Serologic profile of a cohort of pigs and antibody response to an autogenous vaccine for Actinobacillus suis

et al. 2001

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-Actinobacillus suis is a commensal opportunistic pathogen in swine. However, in recent years, an increasing prevalence of clinical signs associated with A. suis has been observed in high health status herds in North America. The objectives of the study were to assess the kinetics of antibodies to A. suis in pigs from a herd showing clinical signs of A. suis infection and, to evaluate the antibody response in gilts following vaccination with an autogenous vaccine. An enzyme-linked immunosorbent assay (ELISA) using a saline extract of boiled-formalinized whole cells of a field strain as the coating antigen was standardized. This ELISA was used as a tool for monitoring, in a comparative way, the variations in A. suis antibody levels. The herd selected for the serologic profile was negative for Actinobacillus pleuropneumoniae infection and showed clinical signs of A. suis infection in 16 to 19-week-old pigs. A cohort of 20 pigs was blood sampled at 5, 8, 12, and 16 weeks of age. The lowest level of serum antibodies was observed between weeks 8 and 12, this probably corresponding to a decrease in maternal immunity. A marked increase in the antibody response was seen at 16-week of age, at the approximate time of onset of A. suis clinical signs in the herd. The evaluation of serum antibody responses to an autogenous vaccine revealed that the humoral immunity of gilts further increased following vaccination although the level of antibodies was already high prior to vaccination. The magnitude of the response to vaccination was higher when the level of antibodies was low prior to the first injection. The ELISA test seems to detect antibodies against the O-chain LPS. Actinobacillus suis / autogenous vaccine / ELISA / serologic profile / pigRésumé -Profil sérologique chez une cohorte de porcs et réponse humorale à un autovaccin pour Actinobacillus suis. Actinobacillus suis est généralement considéré comme un agent pathogène opportuniste chez le porc. Toutefois, en Amérique du Nord, une augmentation de la prévalence des signes cliniques associés à A. suis a été notée récemment dans des troupeaux de haut statut sanitaire. Les objectifs de cette étude étaient d'évaluer la cinétique des anticorps chez des porcs provenant Vet. Res. 32 (2001) 175-183 175

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Section: Serum Antibody Response To An Autogenous Vaccinesupporting

confidence: 79%

Section: Animalsmentioning

confidence: 99%

Section: Elisamentioning

confidence: 99%

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Serologic profile of a cohort of pigs and antibody response to an autogenous vaccine for Actinobacillus suis

et al. 2001

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“…In addition, some Haemophilus parasuis and Actinobacillus suis strains may present cross-reactions with A. pleuropneumoniae. 6,13 The use of serotype-specific monoclonal antibodies (MAbs) could help overcome these problems. In addition, MAbs may be used for a more precise characterization of antigens involved in Received for publication October 14, 1996. the classification of A. pleuropneumoniae.…”

mentioning

confidence: 99%

“…Epitopes recognized by each of the selected serotype-specific MAbs were studied by testing their reactivity with different antigens in ELISA and immunoblot. 6 Different antigens consisted of WCA and O-chain lipopolysaccharide (LPS), purified as previously described. 7 In addition, for serotype 5, purified capsular polysaccharidel was used.…”

mentioning

confidence: 99%