Evaluation of a novel standardized system for collection and quantification of oral fluid

Raggam, Reinhard B.; Santner, Brigitte I.; Kollroser, Manfred; Gössler, Walter; Schmied, Bettina; Schmitt, Ursula; Stelzl, Evelyn; Lackner, Andreas; Wagner, Jasmin; Marth, Egon; Kessler, Harald H.

doi:10.1515/cclm.2008.044

Cited by 17 publications

(12 citation statements)

References 10 publications

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“…However none of these allow direct and accurate volume quantification collected, thus hampering saliva secretion rate estimation ( 34 ). Another available collection system is the Saliva Collection System (SCS) ® (Greiner Bio-One GmgH, Kremsmuenster, Austria) ( 2 , 19 ) which uses a citric acid buffered liquid. This collection system is more elaborate and uses one solution for cleaning oral cavity and another to collect saliva.…”

Section: Saliva Collection Methods and Storagementioning

confidence: 99%

“…This collection system has been recently tested using routine laboratory methods. It has demonstrated reliable and reproducible results for measurement of calcium, magnesium ( 19 ), salivary alpha-amylase (SAA), UA ( 2 ), IgA, cortisol, drug and medication monitoring ( 33 ) and quantification of viral RNA ( 7 ). However, some analytes may suffer interference due to low pH and additives present in the extraction solution ( 2 , 33 ).…”

Section: Saliva Collection Methods and Storagementioning

confidence: 99%

“…Salivary flow rate, composition and protein concentration varies amongst individuals depending on factors such as age, oral microbial enzymes, sample processing, preservation, protease activity etc. The analytical methods should be adapted, allowing high sensitivity and reproducibility ( 19 ). The aim of this review is to highlight the suitable diagnostic value of saliva as a less invasive fluid in health and human systemic diseases.…”

Section: Introductionmentioning

confidence: 99%

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Clinical and diagnostic utility of saliva as a non-invasive diagnostic fluid: a systematic review

2015

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This systematic review presents the latest trends in salivary research and its applications in health and disease. Among the large number of analytes present in saliva, many are affected by diverse physiological and pathological conditions. Further, the non-invasive, easy and cost-effective collection methods prompt an interest in evaluating its diagnostic or prognostic utility. Accumulating data over the past two decades indicates towards the possible utility of saliva to monitor overall health, diagnose and treat various oral or systemic disorders and drug monitoring. Advances in saliva based systems biology has also contributed towards identification of several biomarkers, development of diverse salivary diagnostic kits and other sensitive analytical techniques. However, its utilization should be carefully evaluated in relation to standardization of pre-analytical and analytical variables, such as collection and storage methods, analyte circadian variation, sample recovery, prevention of sample contamination and analytical procedures. In spite of all these challenges, there is an escalating evolution of knowledge with the use of this biological matrix.

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Section: Saliva Collection Methods and Storagementioning

confidence: 99%

Section: Saliva Collection Methods and Storagementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Clinical and diagnostic utility of saliva as a non-invasive diagnostic fluid: a systematic review

2015

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“…To date, there have been few studies quantifying salivary histamine by different collection and measurement methods, which impedes comparison (Hyyppa, 1984; Venza et al , 2006). In our study, stimulated whole saliva was collected by means of a system, which provides a rather reliable and accurate quantification of the recovered saliva volume compared to conventional methods (Raggam et al , 2008; Nunes et al , 2011). The salivary histamine levels of the control group presented a mean value of 17.5 ng/ml.…”

Section: Discussionmentioning

confidence: 99%

Smoking influences salivary histamine levels in periodontal disease

Bertl¹,

Haririan²,

Laky

et al. 2012

Oral Diseases

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Smoking, an established and common risk factor of periodontitis, was assessed as a possible influencing factor for salivary histamine. Most interestingly, salivary histamine differed highly significantly between smoking and non-smoking periodontitis patients. Our results suggest a possible involvement of histamine in tobacco-exacerbated periodontal disease, but do not suggest salivary histamine as a reliable diagnostic marker for periodontitis.

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“…The collected saliva (saliva + extraction solution) is subsequently transferred via vacuum system to graduated transport tubes, which permit to quantify the total volume of collected solution (22,45) . This system of saliva collection has been recently tested using routine laboratory methods and has demonstrated reliable and reproducible results for measurement of calcium, magnesium (49) , α-amylase, uric acid (45) , IgA, cortisol and therapeutic drugs (22) . Nonetheless, certain analytes are prone to interference due to pH and additives present in the extraction solution (45) .…”

Section: Methods For Saliva Collectionmentioning

confidence: 99%

Saliva as a diagnostic fluid in sports medicine: potential and limitations

Nunes¹,

Macedo²

2013

J. Bras. Patol. Med. Lab.

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The use of saliva in the diagnosis of pathologies and/or monitoring of athletes in competitions or trainings is an attractive alternative due to the fact that samples are easily obtained and it is mostly a less invasive method in comparison with venous blood collection. The saliva is a hypotonic fluid in relation to plasma, containing compounds produced in the salivary glands (immunoglobulin A [IgA] and α-amylase) as well as compounds diffused in the plasma (water, electrolytes, proteins, metabolites and hormones). It plays a pivotal role in the protection of oral mucosa against microbes and in food digestion. Its production and composition depend on the sympathetic and parasympathetic nervous system activity, whose antagonistic action may result in different saliva volumes with distinct ionic and protein profiles. The aim of this review was to present a critical analysis of the potential and limitations of saliva as a diagnostic tool in sports medicine. Although there are studies that have deployed it to monitor athletes in training and doping, the standardization of some preanalytical variables are still required, among which the following ones are worth mentioning: the accurate choice of collection system, which allows the easy quantification of volume with adequate sample recovery; well-defined collection schedules in accordance with the circadian variations of the analyte; prevention of sample contamination with blood from oral mucosa lesions. Another key point for its application in sports is the establishment of reference intervals for analytes quantified in the saliva, collected from a population that comprises healthy individual that exercise regularly and systematically, with physical activity progression.

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Evaluation of a novel standardized system for collection and quantification of oral fluid

Abstract: The new system for collection and quantification of oral fluid helps to improve standardization of pre-analytics in oral fluid testing and to provide reliable and accurate quantification of analytes in oral fluid samples.

Cited by 17 publications

References 10 publications

Clinical and diagnostic utility of saliva as a non-invasive diagnostic fluid: a systematic review

Clinical and diagnostic utility of saliva as a non-invasive diagnostic fluid: a systematic review

Smoking influences salivary histamine levels in periodontal disease

Saliva as a diagnostic fluid in sports medicine: potential and limitations

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