Evaluation of a novel rapidly-growing mycobacteria medium for isolation of Mycobacterium abscessus complex from respiratory specimens from patients with bronchiectasis

Molina, Susan; Fly, Travis; Njie, Ousman; Stribley, Patricia; Stephenson, Dominic; Wallace, Richard J.; Perry, John D.

doi:10.1016/j.heliyon.2019.e02684

Cited by 5 publications

(3 citation statements)

References 12 publications

(23 reference statements)

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“…With respect to MAC, there was a lower impact of the various decontamination treatments examined here, with NALC-NaOH reducing the viable count by 28.9%. This is consistent with the fact that there is little, if any, advantage of using RGM medium for isolation of MAC when compared to AFB culture, as documented in previous studies [ 7 , 19 , 20 , 21 ]. A limitation of this paper is that we are not able to offer conclusions on NTMs other than MABSC and MAC.…”

Section: Discussionsupporting

confidence: 91%

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Decontamination Strategies Used for AFB Culture Significantly Reduce the Viability of Mycobacterium abscessus Complex in Sputum Samples from Patients with Cystic Fibrosis

et al. 2021

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Nontuberculous mycobacteria are important respiratory pathogens in patients with cystic fibrosis (CF). For diagnosis, international guidelines recommend culture of sputum that has been decontaminated via chemical treatment. Fifty-six sputum samples from 32 patients known to be previously colonized or infected with NTM were subdivided, and the aliquots were subjected to six different decontamination strategies, followed by quantitative culture for NTM. Thirty sputum samples contained Mycobacterium abscessus complex (MABSC) and 11 contained Mycobacterium avium complex (MAC). Decontamination strategies included treatment with N-acetyl L-cysteine with 2% sodium hydroxide (NALC-NaOH), 4% NaOH, 1% chlorhexidine, 0.5 N sulfuric acid, 5% oxalic acid, double decontamination with NALC-NaOH, followed by 5% oxalic acid, and saline (0.85%) as a control. The samples were also cultured directly with no treatment. Treatment with NALC-NaOH resulted in an average reduction in colony count of 87% for MABSC when compared with direct culture. NaOH at 4% caused a 98.3% average reduction in colony count. All treatments that included NaOH resulted in colony counts that were statistically lower than those obtained from direct culture or the saline-treated control (p < 0.05). Standard treatments using sulfuric or oxalic acids were less deleterious, but still resulted in an average reduction in colony count of at least 30%. The viability of MAC was much less affected by most decontamination treatments. In conclusion, the viability of MABSC was severely compromised by standard decontamination regimens. This supports recent evidence showing that optimal recovery of MABSC is achieved by culture on an appropriate selective agar without decontamination of sputum samples.

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Section: Discussionsupporting

confidence: 91%

“…This effect is particularly acute for treatments containing NaOH against MABSC. This supports the abundance of recently acquired evidence showing that the optimal recovery of MABSC is achieved by culture on an appropriate selective agar, without decontamination of sputum samples [ 7 , 19 , 21 ].…”

Section: Discussionsupporting

confidence: 81%

Decontamination Strategies Used for AFB Culture Significantly Reduce the Viability of Mycobacterium abscessus Complex in Sputum Samples from Patients with Cystic Fibrosis

et al. 2021

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“…However, most NTM can also be isolated with these systems, and criteria for the interpretation of the microbiological results have been developed for a clear interpretation of the isolation of these environmental species [ 58 ]. In the present moment, some specific media for NTM have been developed and have been used, not only for clinical samples, but for environmental ones [ [85] , [86] , [87] ]. These later samples are of special interest, because NTM grow in them as part of polymicrobial biofilms, and because the slow growing of all species (compared with other bacteria) the isolation of them can be problematic.…”

Section: Diagnosismentioning

confidence: 99%