Culturing Mycobacteria

Wallace, Elizabeth; Hendrickson, Debra; Tolli, Nicholas; Mehaffy, Carolina; Peña, María; Nick, Jerry A.; Knabenbaur, Phillip; Watkins, Jackson; Simpson, Anne; Amin, Asif; Chatterjee, Delphi; Dobos, Karen M.; Lahiri, Ramanuj; Adams, Linda B.; Strong, Michael; Bradford, Rebecca; Stedman, Timothy T.; Riojas, Marco A.; Hazbón, Manzour Hernando

doi:10.1007/978-1-0716-1460-0_1

Cited by 14 publications

(8 citation statements)

References 31 publications

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“…Furthermore, mNGS showed greater advantages in identifying M. tuberculosis , nontuberculous mycobacteria, atypical pathogens, viruses, and fungi. The culture cycle of M. tuberculosis is long, and most species of nontuberculous mycobacteria are difficult to culture ( 22 ). Thus, mNGS could be an effective method to identify mycobacteria due to its superior sensitivity ( 23 , 24 ).…”

Section: Discussionmentioning

confidence: 99%

Improving Suspected Pulmonary Infection Diagnosis by Bronchoalveolar Lavage Fluid Metagenomic Next-Generation Sequencing: a Multicenter Retrospective Study

Jin

Shao

et al. 2022

Microbiol Spectr

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Pulmonary infection is a heterogeneous and complex infectious disease with high morbidity and mortality worldwide. In clinical practice, a considerable proportion of the etiology of pulmonary infection is unclear, microbiological diagnosis being challenging. Metagenomic next-generation sequencing detects all nucleic acids in a sample in an unbiased manner, revealing the microbial community environment and organisms and improving the microbiological detection and diagnosis of infectious diseases in clinical settings.

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Section: Discussionmentioning

confidence: 99%

Improving Suspected Pulmonary Infection Diagnosis by Bronchoalveolar Lavage Fluid Metagenomic Next-Generation Sequencing: a Multicenter Retrospective Study

Jin

Shao

et al. 2022

Microbiol Spectr

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“…Most species of mycobacteria are difficult to culture due to their long growth cycles, low sensitivities, and susceptibility to contamination. 26 C. psittaci and O. tsutsugamushi were classified as atypical pathogens and were also difficult to detect by conventional testing. P. jirovecii is a pathogenic fungus that can cause Pneumocystis pneumonia in immunocompromised patients.…”

Section: Discussionmentioning

confidence: 99%

Clinical Efficacy and Diagnostic Value of Metagenomic Next-Generation Sequencing for Pathogen Detection in Patients with Suspected Infectious Diseases: A Retrospective Study from a Large Tertiary Hospital

Xiao¹,

Liu²,

Wu³

et al. 2023

IDR

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Purpose Metagenomic next-generation sequencing (mNGS) is a powerful yet unbiased method to identify pathogens in suspected infections. However, little is known about its clinical effectiveness. The present study aimed to assess the efficacy of mNGS in routine clinical practice. Patients and Methods In this single-center retrospective cohort study, 518 patients with suspected infectious diseases were assessed for inclusion. Among them, each patient had undergone mNGS testing; 407 patients had undergone both microbial culture and mNGS testing. The result of mNGS testing was compared to microbial culture performed concurrently. The diagnostic performance of mNGS was evaluated using the comprehensive clinical diagnosis as the reference standard. Results There was a significant difference in the positive detection rates of pathogens between mNGS and culture (331/407, 81.3% vs 79/407, 19.4%, P < 0.001). The sensitivity of mNGS was much higher than the culture method (79.5% vs 21.3%, P < 0.001), especially in sample types of sputum and bronchoalveolar lavage fluid (BALF). Notably, the sensitivity of blood mNGS was relatively lower than other sample types (67.4% vs 88.9–93.8%). Pathogen cfDNA load based on standardized stringently mapped read number at the species level of microorganisms (SDSMRN) was significantly lower in blood than in other sample types from the same patient ( P = 0.0003). Importantly, mNGS directly led to a change of treatment regimen in 142 (27.4%) cases, including antibiotic escalation (15.3%), antibiotic de-escalation (9.1%), and early definitive diagnosis to initiate appropriate treatment (3.1%). Conclusion Our in-house mNGS platform significantly improved the sensitivity for the diagnosis of infectious diseases. mNGS has the potential to improve clinical outcomes by optimizing antimicrobial therapy.

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“…In this study, pellets from each sample were subjected to treatment and subsequently inoculated into Middlebrook 7H10 solid slopes and liquid culture broth mycobacterial growth indicator tubes (MGIT) to facilitate growth. The cultures were maintained under optimal conditions, i.e., at a temperature of 37 °C for up to 6 weeks and 8 weeks for the liquid and solid media, respectively [6] . Positive identification of mycobacterial isolates was confirmed using Ziehl-Neelsen staining, while biochemical tests, carried out using molecular probes (Accuprobe) were utilized to observe colony morphology and/or nucleic acid hybridization for species identification.…”

Section: Methodsmentioning

confidence: 99%

Comparative analysis of five etiological detecting techniques for the positive rates in the diagnosis of tuberculous granuloma

Liu,

Xu,

Liu

et al. 2023

Journal of Clinical Tuberculosis and Other Mycobacterial Diseas

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Culturing Mycobacteria

Cited by 14 publications

References 31 publications

Improving Suspected Pulmonary Infection Diagnosis by Bronchoalveolar Lavage Fluid Metagenomic Next-Generation Sequencing: a Multicenter Retrospective Study

Improving Suspected Pulmonary Infection Diagnosis by Bronchoalveolar Lavage Fluid Metagenomic Next-Generation Sequencing: a Multicenter Retrospective Study

Clinical Efficacy and Diagnostic Value of Metagenomic Next-Generation Sequencing for Pathogen Detection in Patients with Suspected Infectious Diseases: A Retrospective Study from a Large Tertiary Hospital

Comparative analysis of five etiological detecting techniques for the positive rates in the diagnosis of tuberculous granuloma

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