Evaluation of a Novel Automated Protocol for the Collection of Peripheral Blood Stem Cells Mobilized with Chemotherapy or Chemotherapy Plus G-CSF Using the Fresenius AS104 Cell Separator

Pierelli, Luca; Menichella, G; Paoloni, A.; Vittori, Mariangela; Foddai, Maria Laura; Serafini, R.; Rumi, Carlo; Mitschulat, H.; Rossi, Pier Luigi; Scambia, Giovanni; Teofili, Luciana; Sica, Simona; Leone, Giuseppe; Bizzi, B

doi:10.1089/scd.1.1993.2.145

Cited by 20 publications

(23 citation statements)

References 10 publications

(8 reference statements)

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“…The collection of PBSC can be performed on various cell separator devices operating with semiautomated or automated apheresis programs [9, 10, 11]. The aim of the present study was to evaluate collection yields of conventional-volume COBE Spectra AutoPBSC leukaphereses and to demonstrate clinical significance of collected PBSC following HDCT.…”

Section: Discussionmentioning

confidence: 99%

“…Other reports described CD34-CE between 48 and 87% at preapheresis peripheral blood CD34+ cell counts of approximately 30/µl, using the cell separators Fresenius AS104, Haemonetics MCS-3P, COBE Spectra (protocol 4.7) and/or Fenwal CS3000 [9, 10, 11, 19, 20, 21]. Some authors reported better CD34-CE at a preapheresis level of circulating CD34+ cells <50/µl [13, 22].…”

Section: Discussionmentioning

confidence: 99%

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Automated Collection of Peripheral Blood Stem Cells with the COBE Spectra for Autotransplantation

et al. 2000

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Background: A convenient, effective and safe peripheral blood stem cell (PBSC) apheresis procedure is desirable to cope with the increasing requirements for PBSC collections. We performed PBSC harvesting with the novel COBE Spectra AutoPBSC^TM system using the default software configuration recommended by the manufacturer. We analyzed collection parameters and clinical efficiency of harvested autografts following high-dose chemotherapy (HDCT). Patients and Methods: Eighty-one patients underwent 102 harvests after standard chemotherapy plus filgrastim (5–10 μg/kg/day) to obtain a target of ≥2.5 × 10⁶ CD34+ cells/kg for autologous blood stem cell transplantation. Conventional-volume leukaphereses (median: 11 liters) were performed using the manufacturer’s standard software default regarding inlet flow, harvest/chase volume (3/7 ml) and number of collection cycles. The ratio of ACD-A to whole blood was initially set at 1:12 (56 collections), later at 1:10 (46 aphereses). Results: With respect to preapheresis counts of 93 (9–876) CD34+ cells/μl, 69 patients (85.2%) achieved ≥2.5 × 10⁶ CD34+ cells/kg by the first apheresis. PBSC products contained medians of 5.0 × 10⁶ (0.7–77.3) CD34+ cells/kg and 13.8 × 10⁴ (2.3–105.0) CFU-GM/kg. A preapheresis count of ≥40 CD34+ cells/μl predicted a single-apheresis yield of ≥2.5 × 10⁶ CD34+ cells/kg. Apheresis products showed a high mononuclear cell (MNC) purity of ≥89%. The median overall collection efficiency of CD34+ cells (CD34-CE) was 42.6% (12.2–87.4). The CD34-CE decreased significantly with increasing numbers of circulating CD34+ cells: 52.5% at CD34+ cells <40/μl versus 41.0% at CD34+ cells ≥40/μl (p ≤ 0.002). Coagulation problems occurred in 3 of 56 procedures at an ACD-A to whole blood ratio of 1:12, but in no case at 1:10. Patients who underwent HDCT and autologous blood stem cell transplantation (n = 40) engrafted in a median time of 9 (8–13) days for absolute neutrophil counts >0.5 × 10³/μl, 10 (8–13) days for WBC >1.0 × 10³/μl and 11 (8–17) days for platelets >20 × 10³/μl. Conclusions: As a result of efficient PBSC mobilization, a single conventional-volume leukapheresis with the COBE Spectra AutoPBSC system resulted in hematopoietic autografts with ≥2.5 × 10⁶ CD34+ cells/kg in 85% of patients. Following the standard PBSC apheresis recommendations of the manufacturer, the AutoPBSC system assures PBSC products with a high MNC purity and a moderate CD34-CE that declines significantly at increasing levels of circulating CD34+ cells. Leukaphereses performed at an ACD-A to whole blood ratio of 1:10 should run without coagulation problems.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Automated Collection of Peripheral Blood Stem Cells with the COBE Spectra for Autotransplantation

et al. 2000

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“…The study was approved by the Hospital Human Investigation Review Board and written informed consent was obtained from all patients. Patients underwent PBPC collection by leukapheresis using the Fresenius AS104 (Fresenius, St Wendel, Germany) blood cell separator and a previously described automated collection protocol (Pierelli et al, 1993). The collection procedures were started when CD34 cell concentration exceeded 20 cells/l.…”

Section: Collection Of Human Peripheral Blood Progenitor Cells (Pbpc)mentioning

confidence: 99%

Generation of multinuclear tartrate‐resistant acid phosphatase positive osteoclasts in liquid culture of purified human peripheral blood CD34⁺ progenitors

Pierelli¹,

Scambia²,

D’Onofrio³

et al. 1997

Br J Haematol

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Summary. Circulating CD34 cells were isolated from leukapheresis products collected from patients with ovarian cancer. CD34 ÿ contaminating cells, identified immediately after immunoselection, ranged from 5% to 25% in five different experiments and were predominantly CD3natural killer cells (range 2-11%) and rare mature CD15 / CD11b granulocytes (range 1-2%). CD34 cells were cultured in liquid medium in the presence of interleukin-3, granulocyte-macrophage colony stimulating factor, stem cell factor, granulocyte colony stimulating factor and a powerful proliferation with prevalent differentiation along the granulocytic/monocytic lineage was obtained. After 10 d of culture a small but consistent number of early multinucleated osteoclasts were identified with a frequency of one cell per 700 granulocytic/monocytic cells, as revealed by cytologic examination. This observation was confirmed by staining for tartrate-resistant acid phosphatase activity which revealed red multinucleated elements with a frequency comparable to that reported above. Conversely, no osteoclasts were observed in those cultures in which macrophage overgrowth was obtained by culturing CD34 cells until day 35.These observations suggest that circulating progenitors have a multilineage potential in vitro and contribute to the clarification of osteoclast development in humans; additionally, they provide the basis for the future development of optimized osteoclast culture techniques in liquid medium and the basic culture system, to test the distinct activity of 1,25(OH) 2 D 3 , parathyroid hormone, interleukin-11 and of other cytokines on osteoclast development in humans.

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“…Machine factors influencing the results of the CPC harvests include the type of cell separator used and the flow rate during the leukapheresis [5,6,11,12].…”

Section: Introductionmentioning

confidence: 99%

Adjustment of the interface detector (location 71) to the absolute number of mononuclear cells in the peripheral blood: No improvement of the collection efficiency of the Fenwal CS3000 plus during progenitor cell harvests

Baars¹,

Nooyen²,

Beers³

et al. 1997

J. Clin. Apheresis

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Evaluation of a Novel Automated Protocol for the Collection of Peripheral Blood Stem Cells Mobilized with Chemotherapy or Chemotherapy Plus G-CSF Using the Fresenius AS104 Cell Separator

Cited by 20 publications

References 10 publications

Automated Collection of Peripheral Blood Stem Cells with the COBE Spectra for Autotransplantation

Automated Collection of Peripheral Blood Stem Cells with the COBE Spectra for Autotransplantation

Generation of multinuclear tartrate‐resistant acid phosphatase positive osteoclasts in liquid culture of purified human peripheral blood CD34⁺ progenitors

Adjustment of the interface detector (location 71) to the absolute number of mononuclear cells in the peripheral blood: No improvement of the collection efficiency of the Fenwal CS3000 plus during progenitor cell harvests

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Evaluation of a Novel Automated Protocol for the Collection of Peripheral Blood Stem Cells Mobilized with Chemotherapy or Chemotherapy Plus G-CSF Using the Fresenius AS104 Cell Separator

Cited by 20 publications

References 10 publications

Automated Collection of Peripheral Blood Stem Cells with the COBE Spectra for Autotransplantation

Automated Collection of Peripheral Blood Stem Cells with the COBE Spectra for Autotransplantation

Generation of multinuclear tartrate‐resistant acid phosphatase positive osteoclasts in liquid culture of purified human peripheral blood CD34+ progenitors

Adjustment of the interface detector (location 71) to the absolute number of mononuclear cells in the peripheral blood: No improvement of the collection efficiency of the Fenwal CS3000 plus during progenitor cell harvests

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Generation of multinuclear tartrate‐resistant acid phosphatase positive osteoclasts in liquid culture of purified human peripheral blood CD34⁺ progenitors