Adjustment of the interface detector (location 71) to the absolute number of mononuclear cells in the peripheral blood: No improvement of the collection efficiency of the Fenwal CS3000 plus during progenitor cell harvests

Abstract

Adjustment of the interface detector (location 71) to the absolute number of mononuclear cells in the peripheral blood: no improvement of the collection efficiency of the Fenwal CS3000 olus during progenitor cell harvests.

Improved collection of mobilized CD34+ hematopoietic progenitor cells by a novel automated leukapheresis system

“…This CD34+ cell dose not only influences the engraftment kinetics, as patients receiving higher CD34+ cell doses appear to have better hematologic measures at later times after transplantation, 13 but it also facilitates allogeneic transplantation across HLA barriers. In this context, we used the CD34+ cell CE (and not MNC CE) together with the CD34+ cell yield in the component to evaluate the new apheresis technique, because CD34+ cell counts correlate with the engraftment kinetics more closely than MNC counts do, and CD34+ cell CE does not always correlate with MNC CE 14 . On the basis of the favorable low platelet CEs in the AutoPBSC shown in this article, our group has changed the criteria for starting leukapheresis from 70,000 platelets per μL 15 to ≥25,000 platelets per μL, which allows us to carry out successful collection of CD34+ HPCs in heavily treated “poor‐mobilizer” and thrombocytopenic patients.…”

Clinically relevant hypokalaemia, hypocalcaemia, and loss of hemoglobin and platelets during stem cell apheresis

“…Different cell densities amongst the leukocyte population allow the collection of the mononuclear cell fraction by means of centrifugation and separation. Optimal adjustment of the cell separator increases the collection efficiency and purity of the apheresis products [7,8]. Nevertheless, it is well known that the purity of mononuclear cells is limited by the contamination of platelets, neutrophil granulocytes and erythrocytes.…”