Improved collection of mobilized CD34+ hematopoietic progenitor cells by a novel automated leukapheresis system

Ravagnani, Fernando; Siena, Salvatore; Reys, S De; Nicola, Massimo Di; Notti, P.; Giardini, Roberto; Bregni, Marco; Matteucci, Paola; Am, Gianni; Pellegris, G

doi:10.1046/j.1537-2995.1999.39199116894.x

Cited by 46 publications

(63 citation statements)

References 27 publications

(39 reference statements)

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“…The calculated results for the CE (mean of 84.4%) were comparable to other studies who used the same cell separator, but partially different software versions for autologous PBPC collections. 30,[32][33][34] The recruitment of stem cells has been described as a phenomenon whereby the leukapheresis procedure itself exerts a stimulatory effect on the release of CD34 ϩ cells into the circulation. 16 Hillyer et al 7 first postulated a recruitment of PBPC during LVL in normal donors resulting in a more than two-fold increase of CFU-GM.…”

Section: Discussionmentioning

confidence: 99%

Kinetics of standardized large volume leukapheresis (LVL) in patients do not show a recruitment phenomenon of peripheral blood progenitor cells (PBPC)

Cassens

Momkvist

Zuehlsdorf

et al. 2001

Bone Marrow Transplant

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Summary:Although several studies have demonstrated the efficacy of large volume leukapheresis (LVL) to yield high numbers of peripheral blood progenitor cells (PBPC), the mechanisms of stem cell release into circulation and the postulated phenomenon of PBPC recruitment during apheresis have not been investigated in detail. Therefore, we analyzed the kinetics of stem cell enrichment in a total of 34 standardized LVL for patients with hematologic malignancies (lymphoma, multiple myeloma) and solid tumors (breast cancer, rhabdomyosarcoma). LVL was started 2 h after administration of G-CSF processing six times the patient's blood volume. Cells were sequentially collected into six bags and the numbers of leukocytes, mononuclear cells (MNC), CD34 ؉ cells and colony-forming cells (CFU-GM) in each collection bag were analyzed. The numbers of PBPC collected demonstrated a continuous decrease starting after an early maximum during the second processed blood volume (P ‫؍‬ 0.001). Interestingly, these kinetics of decreasing stem cell yields during LVL were similar for both entities of patients with hematologic malignancies as well as for both groups of patients with solid tumors. In summary, a recruitment phenomenon, defined as a timedependent and LVL-induced increase of PBPC, could not be demonstrated in any of the diseases investigated. Bone Marrow Transplantation (2001) 28, 13-20.

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Section: Discussionmentioning

confidence: 99%

Kinetics of standardized large volume leukapheresis (LVL) in patients do not show a recruitment phenomenon of peripheral blood progenitor cells (PBPC)

Cassens

Momkvist

Zuehlsdorf

et al. 2001

Bone Marrow Transplant

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“…Ravagnani et al [17]and Schreiner et al [18]described CD34-CE of 68–83% and 52.8%. However, in contrast to our study, they made a number of substantial modifications of the AutoPBSC software default.…”

Section: Discussionmentioning

confidence: 99%

Automated Collection of Peripheral Blood Stem Cells with the COBE Spectra for Autotransplantation

et al. 2000

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Background: A convenient, effective and safe peripheral blood stem cell (PBSC) apheresis procedure is desirable to cope with the increasing requirements for PBSC collections. We performed PBSC harvesting with the novel COBE Spectra AutoPBSC^TM system using the default software configuration recommended by the manufacturer. We analyzed collection parameters and clinical efficiency of harvested autografts following high-dose chemotherapy (HDCT). Patients and Methods: Eighty-one patients underwent 102 harvests after standard chemotherapy plus filgrastim (5–10 μg/kg/day) to obtain a target of ≥2.5 × 10⁶ CD34+ cells/kg for autologous blood stem cell transplantation. Conventional-volume leukaphereses (median: 11 liters) were performed using the manufacturer’s standard software default regarding inlet flow, harvest/chase volume (3/7 ml) and number of collection cycles. The ratio of ACD-A to whole blood was initially set at 1:12 (56 collections), later at 1:10 (46 aphereses). Results: With respect to preapheresis counts of 93 (9–876) CD34+ cells/μl, 69 patients (85.2%) achieved ≥2.5 × 10⁶ CD34+ cells/kg by the first apheresis. PBSC products contained medians of 5.0 × 10⁶ (0.7–77.3) CD34+ cells/kg and 13.8 × 10⁴ (2.3–105.0) CFU-GM/kg. A preapheresis count of ≥40 CD34+ cells/μl predicted a single-apheresis yield of ≥2.5 × 10⁶ CD34+ cells/kg. Apheresis products showed a high mononuclear cell (MNC) purity of ≥89%. The median overall collection efficiency of CD34+ cells (CD34-CE) was 42.6% (12.2–87.4). The CD34-CE decreased significantly with increasing numbers of circulating CD34+ cells: 52.5% at CD34+ cells <40/μl versus 41.0% at CD34+ cells ≥40/μl (p ≤ 0.002). Coagulation problems occurred in 3 of 56 procedures at an ACD-A to whole blood ratio of 1:12, but in no case at 1:10. Patients who underwent HDCT and autologous blood stem cell transplantation (n = 40) engrafted in a median time of 9 (8–13) days for absolute neutrophil counts >0.5 × 10³/μl, 10 (8–13) days for WBC >1.0 × 10³/μl and 11 (8–17) days for platelets >20 × 10³/μl. Conclusions: As a result of efficient PBSC mobilization, a single conventional-volume leukapheresis with the COBE Spectra AutoPBSC system resulted in hematopoietic autografts with ≥2.5 × 10⁶ CD34+ cells/kg in 85% of patients. Following the standard PBSC apheresis recommendations of the manufacturer, the AutoPBSC system assures PBSC products with a high MNC purity and a moderate CD34-CE that declines significantly at increasing levels of circulating CD34+ cells. Leukaphereses performed at an ACD-A to whole blood ratio of 1:10 should run without coagulation problems.

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“…Mononuclear cells were harvested with use of a novel automated leukapheresis system (Auto PBSC Spectra; COBE, Lakewood, CO), as previously described. 16 To hasten hematopoietic recovery and to reduce hematologic toxicity, each patient received 3 progenitor/stem cell reinfusions, both after the severely myelotoxic, yet nonmyeloablative course of high-dose cytarabine (reinfusion 1, Figure 1), and after the 2 subsequent myeloablative courses of melphalan and mitoxantrone plus melphalan (reinfusion 2 and 3, respectively). The minimum target dose of CD34 ϩ cells per kg of body weight to be reinfused after each of the 3 autografts were 2 ϫ 10 6 , 3 ϫ 10 6 , and 5 ϫ 10 6 , respectively.…”

Section: Treatment Schedulementioning

confidence: 99%

Long-term remission in mantle cell lymphoma following high-dose sequential chemotherapy and in vivo rituximab-purged stem cell autografting (R-HDS regimen)

Gianni

Magni

Martelli³

et al. 2003

Blood

187

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Improved collection of mobilized CD34+ hematopoietic progenitor cells by a novel automated leukapheresis system

Abstract: The AutoPBSC offers the following advantages over the V4.7 system: a) better CE of CD34+ cells; b) reduced collection of platelets; c) reduced contamination of the leukapheresis component with granulocytes, platelets, and RBCs; d) reduced component volume; and e) automation.

Cited by 46 publications

References 27 publications

Kinetics of standardized large volume leukapheresis (LVL) in patients do not show a recruitment phenomenon of peripheral blood progenitor cells (PBPC)

Kinetics of standardized large volume leukapheresis (LVL) in patients do not show a recruitment phenomenon of peripheral blood progenitor cells (PBPC)

Automated Collection of Peripheral Blood Stem Cells with the COBE Spectra for Autotransplantation

Long-term remission in mantle cell lymphoma following high-dose sequential chemotherapy and in vivo rituximab-purged stem cell autografting (R-HDS regimen)

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